pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

LOCUS       pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX           8765 bp DNA     circular SYN 21-APR-2023
DEFINITION  Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Dong A, He K, Dudok B, Farrell JS, Guan W, Liput DJ, Puhl HL, Cai R,
            Duan J, Albarran E, Ding J, Lovinger DM, Li B, Soltesz I, Li Y
  TITLE     A fluorescent sensor for spatiotemporally resolved endocannabinoid 
            dynamics in vitro and in vivo
  JOURNAL   bioRxiv October 20, 2020
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            October 20, 2020"
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     737..799
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             824..2953
                     /codon_start=1
                     /label=GRAB_eCBmut
                     /translation="TKSILDGLADTTFRTITTDLLYVGSNDIQYEDIKGDMASKLGYFP
                     QKFPLTSFRGSPFQEKMTAGDNPQLVPADQVNITEFYNKSLSSFKENEENIQCGENFMD
                     IECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSRSLRCRPSYHFIGSLAVAD
                     LLGSVIFVYSFIDAHVFHRKDSRNVFLFKLGGVTASFTASVGSLFLTAIDRYISIHRPL
                     AYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIFPHIDETYLMFWIG
                     VTSVLLLFIVYAYMYILWKAHSHAVRMIQRTDALDLEEGGNVYIKADKQKNGIKANFCI
                     RHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTA
                     AGITFGMDELYKGGTGGSMVRKGEELFTGVVPILVELDGDVNGHKFSVGGEGEGDATVG
                     KLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTGAAARWRGRRMDI
                     RLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCTMLCLLNSTVNPIIYA
                     LRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAESCIKSTVKIA
                     KVTMSVSTDTSAEAL"
     primer_bind     1219..1240
                     /label=WKG148-F1
     primer_bind     1803..1822
                     /label=WKG148-F3
     primer_bind     1993..2014
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2120..2141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2381..2400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2880..2904
                     /label=WKG148-F2
     misc_feature    2964..3549
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3131..3148)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3357..3376
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3550..4257
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3695..3713)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3963..3982
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4315..4344
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4348..4494
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4516..4533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4522..4746
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4878..5466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4958..4977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5795..5842
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5867..5886
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6074..6868)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTYGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6185..6204)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6795..6814
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6967..6986)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7213..7233
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(7316..8176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7939..7958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8177..8281)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8308..8763
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8395..8414)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8605..8626
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccctgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgg
      721 cttggggata tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt
      781 tccaggttcc actggtgaca caagtttgta caaaaaagtt ggcaccaagt cgatcctaga
      841 tggccttgca gataccacct tccgcaccat caccactgac ctcctgtacg tgggctcaaa
      901 tgacattcag tacgaagaca tcaaaggtga catggcatcc aaattagggt acttcccaca
      961 gaaattccct ttaacttcct ttaggggaag tcccttccaa gagaagatga ctgcgggaga
     1021 caacccccag ctagtcccag cagaccaggt gaacattaca gaattttaca acaagtctct
     1081 ctcgtccttc aaggagaatg aggagaacat ccagtgtggg gagaacttca tggacataga
     1141 gtgtttcatg gtcctgaacc ccagccagca gctggccatt gcagtcctgt ccctcacgct
     1201 gggcaccttc acggtcctgg agaacctcct ggtgctgtgc gtcatcctcc actcccgcag
     1261 cctccgctgc aggccttcct accacttcat cggcagcctg gcggtggcag acctcctggg
     1321 gagtgtcatt tttgtctaca gcttcattga cgcccacgtg ttccaccgca aagatagccg
     1381 caacgtgttt ctgttcaaac tgggtggggt cacggcctcc ttcactgcct ccgtgggcag
     1441 cctgttcctc acagccatcg acaggtacat atccattcac aggcccctgg cctataagag
     1501 gattgtcacc aggcccaagg ccgtggtggc gttttgcctg atgtggacca tagccattgt
     1561 gatcgccgtg ctgcctctcc tgggctggaa ctgcgagaaa ctgcaatctg tttgctcaga
     1621 cattttccca cacattgatg aaacctacct gatgttctgg atcggggtca ccagcgtact
     1681 gcttctgttc atcgtgtatg cgtacatgta tattctctgg aaggctcaca gccacgccgt
     1741 ccgcatgatt cagcgtaccg acgcgctgga cctggaggag ggaggaaacg tctatatcaa
     1801 ggccgacaag cagaagaacg gcatcaaggc gaacttctgc atccgccaca acatcgagga
     1861 cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt
     1921 gctgctgccc gacaaccact acctgagcgt gcagtccaaa ctttcgaaag accccaacga
     1981 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctttcggcat
     2041 ggacgagctg tacaagggcg gtaccggagg gagcatggtg agaaagggcg aggagctgtt
     2101 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2161 cgtgggcggc gagggtgagg gcgatgccac cgttggcaag ctgaccctga agttcatctg
     2221 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2281 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2341 gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2401 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2461 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaaca ccggagcagc
     2521 agcacgctgg cgcgggcggc gcatggacat taggttagcc aagaccctgg tcctgatcct
     2581 ggtggtgttg atcatctgct ggggccctct gcttgcaatc atggtgtatg atgtctttgg
     2641 gaagatgaac aagctcatta agacggtgtt tgcattctgc accatgctct gcctgctgaa
     2701 ctccaccgtg aaccccatca tctatgctct gaggagtaag gacctgcgac acgctttccg
     2761 gagcatgttt ccctcttgtg aaggcactgc gcagcctctg gataacagca tgggggactc
     2821 ggactgcctg cacaaacacg caaacaatgc agccagtgtt cacagggccg cagaaagctg
     2881 catcaagagc acggtcaaga ttgccaaggt aaccatgtct gtgtccacag acacgtctgc
     2941 cgaggctctg tgataagcta gcgcccctct ccctcccccc cccctaacgt tactggccga
     3001 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3061 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     3121 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     3181 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     3241 cccccacctg gcgacaggtg cctctgcggc caacgccacg tgtataagat acacctgcaa
     3301 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     3361 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     3421 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     3481 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     3541 gccacaacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     3601 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     3661 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     3721 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     3781 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     3841 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     3901 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     3961 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     4021 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     4081 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     4141 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     4201 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggat
     4261 ggtaaaaaga agaaaaagaa gtcaaagaca aagtgtgtaa ttatgtaagt cgacgaacaa
     4321 aaactcatct cagaagagga tctgaatgct gtgggccagg acacgcagga ggtcatcgtg
     4381 gtgccacact ccttgccctt taaggtggtg gtgatctcag ccatcctggc cctggtggtg
     4441 ctcaccatca tctcccttat catcctcatc atgctttggc agaagaagcc acgttaggcg
     4501 gccgctcgag atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4741 ctatggcttc tgaggcggaa agaaccagtg gcggtaatac ggttatccac agaatcgggg
     4801 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4861 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4921 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4981 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5041 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5101 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5161 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacgcga cttatcgcca
     5221 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5281 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5341 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5401 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5461 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5521 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5581 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aacctgaggc tatggcaggg
     5641 cctgccgccc cgacgttggc tgcgagccct gggccttcac ccgaacttgg ggggtggggt
     5701 ggggaaaagg aagaaacgcg ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca
     5761 gagtgccagc cctgggaccg aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt
     5821 ttattctgtc tttttattgc cgtcatagcg cgggttcctt ccggtattgt ctccttccgt
     5881 gtttcagtta gcctccccct agggtgggcg aagaactcca gcatgagatc cccgcgctgg
     5941 aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg
     6001 gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac
     6061 cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
     6121 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
     6181 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
     6241 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
     6301 cgccataggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca
     6361 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
     6421 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
     6481 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
     6541 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
     6601 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
     6661 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct
     6721 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
     6781 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
     6841 ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga
     6901 tctttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     6961 gccgggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     7021 cggaactggg cggagttagg ggcggaatgg gcggagttag gggcgggact atggttgctg
     7081 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     7141 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     7201 gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact
     7261 cttccttttt caataaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7621 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7921 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8281 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg
     8341 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8401 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8461 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8521 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8581 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8641 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8701 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8761 tttac
//