pYK374

LOCUS       pYK374                7846 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..984
                     /label=oCW60
     primer_bind     881..989
                     /label=oYK209_FUS_F
     primer_bind     881..928
                     /label=oYK449_NLS3_F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     promoter        881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     887..904
                     /label=oYK661_Nhe_F
     primer_bind     902..921
                     /label=oYK809_NLS_F
     misc_feature    905..964
                     /label=NLS
     primer_bind     964..989
                     /label=oYK374_FUS_F
     primer_bind     964..984
                     /label=oCW41
     CDS             965..1609
                     /codon_start=1
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /product="RNA-binding protein FUS isoform 1"
                     /label=FUS
                     /note="isoform 1 is encoded by transcript variant 1; 
                     heterogeneous nuclear ribonucleoprotein P2; fused in 
                     sarcoma; fusion gene in myxoid liposarcoma; 75 kDa 
                     DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
                     fus-like protein; oncogene FUS; translocated in liposarcoma
                     protein"
                     /db_xref="CCDS:CCDS10707.1"
                     /db_xref="GeneID:2521"
                     /db_xref="HGNC:HGNC:4010"
                     /db_xref="MIM:137070"
                     /protein_id="NP_004951.1"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGG"
     primer_bind     965..991
                     /label=oYK397_FUS_F
     primer_bind     965..989
                     /label=oYK525_FUS_F
     primer_bind     968..993
                     /label=oYK339_FUS_F
     misc_feature    1040..1042
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1052..1054
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1088..1090
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine, by ATM. 
                     /evidence=ECO:0000269|PubMed:18620545; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); phosphorylation site"
     misc_feature    1487..1489
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Breakpoint for translocation to form 
                     chimericFUS/ATF1 protein; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); other site"
     primer_bind     complement(1579..1611)
                     /label=oCW42
     primer_bind     complement(1579..1611)
                     /label=oCW61
     primer_bind     1590..1631
                     /label=oYK68_FUS2_F
     primer_bind     1590..1609
                     /label=oYK69_AG_ANR1_F
     primer_bind     complement(1594..1609)
                     /label=oYK68_FUS1-R
     misc_feature    1610..2398
                     /label=FUS215-478
     primer_bind     1698..1712
                     /label=oYK68_FUS2_F
     primer_bind     complement(2377..2418)
                     /label=oYK88_FUS478_R
     misc_feature    2399..2623
                     /label=Long_Linker
     primer_bind     2399..2418
                     /label=oYK78_AG_ANR1_F
     primer_bind     2399..2416
                     /label=oYK69_AG_ANR1_F
     primer_bind     complement(2603..2623)
                     /label=oYK81_ANR_AG_R
     primer_bind     2604..2641
                     /label=oYK1309_EGFP_F
     primer_bind     complement(2622..2638)
                     /label=oCW61
     CDS             2624..3340
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2624..2641
                     /label=oYK01-l-F
     regulatory      2624..2627
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2669..2690)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2930..2949)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3277..3298
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3320..3363)
                     /label=oYK06-ANR-mEGFP-R
                     /note="cacactggactagtggatccTTActtgtacagctcgtccatgcc same as
                     oYK05-mcherry-R"
     primer_bind     complement(3344..3366)
                     /label=oYK57-MCP-mCherry-R
     terminator      3440..3667
                     /label=bGH PA
     misc_feature    3713..4141
                     /label=f1 origin
     misc_feature    4146..4489
                     /label=SV40 early promoter and origin
     misc_feature    4335..4412
                     /label=SV40 ORI
     CDS             4554..5345
                     /label=Kan/neoR
     misc_feature    5519..5649
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5685..5705)
                     /label=M13-rev
     misc_binding    complement(5711..5733)
                     /label=LacO
     promoter        complement(5738..5767)
                     /label=lac
     primer_bind     5882..5893
                     /label=oYK69_AG_ANR1_F
     misc_feature    complement(6032..6705)
                     /label=pUC origin
     CDS             complement(6853..7512)
                     /label=AmpR
     promoter        complement(7752..7780)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac
     1021 ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag
     1081 tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg
     1141 ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg
     1201 cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct cctaccctgg
     1261 ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag
     1321 cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca
     1381 gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa
     1441 ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta actatggcca
     1501 agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca atcaagacca
     1561 gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggca gaggcagagg
     1621 aggaagcgga ggaggaggag ggggaggagg aggcggatac aacagaagca gcggaggata
     1681 cgagccaaga ggaagaggag gaggaagagg agggagagga ggaatgggag gaagcgacag
     1741 aggaggattc aacaagttcg gaggaccaag agaccaggga agcagacacg acagcgagca
     1801 ggacaacagc gacaacaaca caatcttcgt gcagggactg ggcgagaatg tgaccatcga
     1861 gagcgtggcc gactacttca agcagatcgg aatcatcaag acaaacaaga aaacaggaca
     1921 gcccatgatc aacctgtaca ccgacagaga gactggcaag ctgaagggag aggccaccgt
     1981 gagcttcgac gaccccccca gcgccaaagc cgccatcgac tggttcgacg gcaaggagtt
     2041 cagcggcaac ccaatcaaag tgagctttgc caccagaaga gccgacttca acagaggcgg
     2101 aggcaacgga agaggcggaa gaggaagagg ggggccaatg ggcagaggag ggtatggcgg
     2161 aggaggaagc gggggaggag ggagaggggg attcccaagc ggaggagggg ggggaggagg
     2221 ccagcagaga gctggagact ggaagtgccc aaacccaaca tgcgagaaca tgaacttcag
     2281 ctggagaaac gagtgcaacc agtgcaaggc cccaaagcca gatggacccg gcggcggacc
     2341 aggaggaagc catatgggcg gcaactacgg cgacgacaga aggggcggca gaggcggagg
     2401 ttctgggagt ggcggtagtg gtgctggagg cagcgcaggc tctggagcag gcggcagtgc
     2461 cggaagtggc gctggagggt ctgccggatc tggagccggt ggttctgccg gctccggcgc
     2521 tggtgggagc gcttccggaa gtagtcccgg taattccgct gacggcggcg gaggatcggg
     2581 tggtagtggt ggttcaggag gaggatcgac ccaaggagga tccatggtga gcaagggcga
     2641 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2701 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2761 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     2821 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2881 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2941 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3001 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3061 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3121 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3181 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3241 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3301 cgccgccggg atcactctcg gcatggacga gctgtacaag taaggatcca ctagtccagt
     3361 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     3421 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3481 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3541 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3601 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3661 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     3721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3961 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4021 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4081 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4141 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4201 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4321 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4381 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4441 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4501 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4561 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4621 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4681 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4741 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4801 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4861 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4921 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4981 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5041 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5101 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5161 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5221 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5281 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5341 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5401 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5461 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5521 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5581 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5641 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     5701 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5761 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5821 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5881 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//