psiCHECK-2-h1-cluster23

LOCUS       psiCHECK-2-h1-cluster23            6757 bp DNA     circular SYN 02-JUN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2156
                     /label=h1-cluster23
     polyA_signal    2172..2220
                     /note="synthetic polyadenylation signal"
     promoter        2229..2980
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3010..3019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3016..4668
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3975..3983
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4712..4833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4966..5070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5071..5931
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6102..6690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 tttgtcacac aaaagataaa ttgtgctttc ttctttattt caagtaaaga atccttacaa
     1741 ttctcatagc attccatcta cagagggtta taatttcccc agatgtgact aaaatggcca
     1801 tagttatcat taggttttat atcttgtctg aatccattta ggctactata acaagatacc
     1861 ataaactggg tggctcataa acaacaaaaa tttatttctc acagttctgg aagctgagaa
     1921 gtctgagatg aaggtgccag caaatttagt gtctgatgaa gtccgcttac tggctcatgg
     1981 tcttttcacc atatcctcac atgtcagacg gggcaaagga attctctctg gcctctttta
     2041 caaggactct catcccattc aggaagactc agccctcatc acctaatcac ctcccattgg
     2101 cctcacctcc taataccatc accttgggag ttaggatttc aatgtatgaa ttctgcgcgg
     2161 ccgctggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     2221 aggatctaaa tgagtcttcg gacctcgcgg gggccgctta agcggtggtt agggtttgtc
     2281 tgacgcgggg ggagggggaa ggaacgaaac actctcattc ggaggcggct cggggtttgg
     2341 tcttggtggc cacgggcacg cagaagagcg ccgcgatcct cttaagcacc cccccgccct
     2401 ccgtggaggc gggggtttgg tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt
     2461 cgcgcgcccc agagtgtgac cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc
     2521 ggcggcgacg ggctcgctgg gtcctaggct ccatggggac cgtatacgtg gacaggctct
     2581 ggagcatccg cacgactgcg gtgatattac cggagacctt ctgcgggacg agccgggtca
     2641 cgcggctgac gcggagcgtc cgttgggcga caaacaccag gacggggcac aggtacacta
     2701 tcttgtcacc cggaggcgcg agggactgca ggagcttcag ggagtggcgc agctgcttca
     2761 tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg
     2821 tctttagttc tatgatgaca caaaccccgc ccagcgtctt gtcattggcg aattcgaaca
     2881 cgcagatgca gtcggggcgg cgcggtccca ggtccacttc gcatattaag gtgacgcgtg
     2941 tggcctcgaa caccgagcga ccctgcagcg acccgcttaa aagcttggca ttccggtact
     3001 gttggtaaag ccaccatggc cgatgctaag aacattaaga agggccctgc tcccttctac
     3061 cctctggagg atggcaccgc tggcgagcag ctgcacaagg ccatgaagag gtatgccctg
     3121 gtgcctggca ccattgcctt caccgatgcc cacattgagg tggacatcac ctatgccgag
     3181 tacttcgaga tgtctgtgcg cctggccgag gccatgaaga ggtacggcct gaacaccaac
     3241 caccgcatcg tggtgtgctc tgagaactct ctgcagttct tcatgccagt gctgggcgcc
     3301 ctgttcatcg gagtggccgt ggcccctgct aacgacattt acaacgagcg cgagctgctg
     3361 aacagcatgg gcatttctca gcctaccgtg gtgttcgtgt ctaagaaggg cctgcagaag
     3421 atcctgaacg tgcagaagaa gctgcctatc atccagaaga tcatcatcat ggactctaag
     3481 accgactacc agggcttcca gagcatgtac acattcgtga catctcatct gcctcctggc
     3541 ttcaacgagt acgacttcgt gccagagtct ttcgacaggg acaaaaccat tgccctgatc
     3601 atgaacagct ctgggtctac cggcctgcct aagggcgtgg ccctgcctca tcgcaccgcc
     3661 tgtgtgcgct tctctcacgc ccgcgaccct attttcggca accagatcat ccccgacacc
     3721 gctattctga gcgtggtgcc attccaccac ggcttcggca tgttcaccac cctgggctac
     3781 ctgatttgcg gctttcgggt ggtgctgatg taccgcttcg aggaggagct gttcctgcgc
     3841 agcctgcaag actacaaaat tcagtctgcc ctgctggtgc caaccctgtt cagcttcttc
     3901 gctaagagca ccctgatcga caagtacgac ctgtctaacc tgcacgagat tgcctctggc
     3961 ggcgccccac tgtctaagga ggtgggcgaa gccgtggcca agcgctttca tctgccaggc
     4021 atccgccagg gctacggcct gaccgagaca accagcgcca ttctgattac cccagagggc
     4081 gacgacaagc ctggcgccgt gggcaaggtg gtgccattct tcgaggccaa ggtggtggac
     4141 ctggacaccg gcaagaccct gggagtgaac cagcgcggcg agctgtgtgt gcgcggccct
     4201 atgattatgt ccggctacgt gaataaccct gaggccacaa acgccctgat cgacaaggac
     4261 ggctggctgc actctggcga cattgcctac tgggacgagg acgagcactt cttcatcgtg
     4321 gaccgcctga agtctctgat caagtacaag ggctaccagg tggccccagc cgagctggag
     4381 tctatcctgc tgcagcaccc taacattttc gacgccggag tggccggcct gcccgacgac
     4441 gatgccggcg agctgcctgc cgccgtcgtc gtgctggaac acggcaagac catgaccgag
     4501 aaggagatcg tggactatgt ggccagccag gtgacaaccg ccaagaagct gcgcggcgga
     4561 gtggtgttcg tggacgaggt gcccaagggc ctgaccggca agctggacgc ccgcaagatc
     4621 cgcgagatcc tgatcaaggc taagaaaggc ggcaagatcg ccgtgtaata attctagagt
     4681 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     4741 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4801 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4861 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     4921 ggtaaaatcg ataaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6721 ctggcctttt gctggccttt tgctcacatg gctcgac
//