psiCHECK-2-SYT1wt

LOCUS      psiCHECK-2-SYT1wt             7550 bp DNA     circular SYN 21-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1681..2949
                     /codon_start=1
                     /label=SYT1wt
                     /translation="MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKE
                     KFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINM
                     KDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGI
                     IQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGG
                     KTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSL
                     RYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYY
                     NESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPR
                     RPIAQWHTLQVEEEVDAMLAVKK"
     misc_feature    2782..2784
                     /label=Ile
     polyA_signal    2965..3013
                     /note="synthetic polyadenylation signal"
     promoter        3022..3773
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3803..3812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3809..5461
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             4768..4776
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    5505..5626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5759..5863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5864..6724
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6895..7483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 atggtgagcg agagtcacca tgaggccctg gcagccccgc ctgtcaccac tgtcgcgact
     1741 gttctgccaa gcaatgccac agagccagcc agtcctggag aaggaaagga agatgcattt
     1801 tctaagctga aggagaagtt tatgaatgag ttgcataaaa ttccattgcc accgtgggcc
     1861 ttaattgcaa tagccatagt cgcagtcctt ttagtcctga cctgctgctt ttgtatctgt
     1921 aagaaatgtt tgttcaaaaa gaaaaacaag aagaagggaa aggaaaaagg agggaagaat
     1981 gccattaaca tgaaagatgt aaaagactta gggaagacga tgaaagatca ggccctcaag
     2041 gatgatgatg ctgaaactgg attgacagat ggagaagaaa aagaagaacc caaagaagag
     2101 gagaaactgg gaaaacttca gtattcactg gattatgatt tccaaaataa ccagctgctg
     2161 gtagggatca ttcaggctgc cgaactgccc gccttggaca tggggggcac atctgatcct
     2221 tacgtgaaag tgtttctgct acctgataag aagaagaaat ttgagacaaa agtccaccga
     2281 aaaaccctta atcctgtctt caatgagcaa tttactttca aggtaccata ctcggaattg
     2341 ggtggcaaaa ccctagtgat ggctgtatat gattttgatc gtttctctaa gcatgacatc
     2401 attggagaat ttaaagtccc tatgaacaca gtggattttg gccatgtaac tgaggaatgg
     2461 cgtgacctgc aaagtgctga gaaggaagag caagagaaat tgggtgatat ctgcttctcc
     2521 cttcgctacg tacctactgc tggtaagctg actgttgtca ttctggaggc aaagaacctg
     2581 aagaagatgg atgtgggtgg cttatccgat ccttatgtga agattcatct gatgcagaat
     2641 ggtaagaggc tgaagaagaa aaagacaaca attaaaaaga acacacttaa cccctactac
     2701 aatgagtcat tcagctttga agtacctttt gaacaaatcc agaaagtgca ggtggtggta
     2761 actgttttgg actatgacaa gattggcaag aacgatgcca tcggcaaagt ctttgtgggc
     2821 tacaacagca ccggcgcgga gctgcgacac tggtcagaca tgctggccaa ccccaggcga
     2881 cctattgccc agtggcacac cctgcaggta gaggaggaag ttgatgccat gctggccgtc
     2941 aagaagtaag cggccgctgg ccgcaataaa atatctttat tttcattaca tctgtgtgtt
     3001 ggttttttgt gtgaggatct aaatgagtct tcggacctcg cgggggccgc ttaagcggtg
     3061 gttagggttt gtctgacgcg gggggagggg gaaggaacga aacactctca ttcggaggcg
     3121 gctcggggtt tggtcttggt ggccacgggc acgcagaaga gcgccgcgat cctcttaagc
     3181 acccccccgc cctccgtgga ggcgggggtt tggtcggcgg gtggtaactg gcgggccgct
     3241 gactcgggcg ggtcgcgcgc cccagagtgt gaccttttcg gtctgctcgc agacccccgg
     3301 gcggcgccgc cgcggcggcg acgggctcgc tgggtcctag gctccatggg gaccgtatac
     3361 gtggacaggc tctggagcat ccgcacgact gcggtgatat taccggagac cttctgcggg
     3421 acgagccggg tcacgcggct gacgcggagc gtccgttggg cgacaaacac caggacgggg
     3481 cacaggtaca ctatcttgtc acccggaggc gcgagggact gcaggagctt cagggagtgg
     3541 cgcagctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
     3601 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
     3661 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
     3721 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taaaagcttg
     3781 gcattccggt actgttggta aagccaccat ggccgatgct aagaacatta agaagggccc
     3841 tgctcccttc taccctctgg aggatggcac cgctggcgag cagctgcaca aggccatgaa
     3901 gaggtatgcc ctggtgcctg gcaccattgc cttcaccgat gcccacattg aggtggacat
     3961 cacctatgcc gagtacttcg agatgtctgt gcgcctggcc gaggccatga agaggtacgg
     4021 cctgaacacc aaccaccgca tcgtggtgtg ctctgagaac tctctgcagt tcttcatgcc
     4081 agtgctgggc gccctgttca tcggagtggc cgtggcccct gctaacgaca tttacaacga
     4141 gcgcgagctg ctgaacagca tgggcatttc tcagcctacc gtggtgttcg tgtctaagaa
     4201 gggcctgcag aagatcctga acgtgcagaa gaagctgcct atcatccaga agatcatcat
     4261 catggactct aagaccgact accagggctt ccagagcatg tacacattcg tgacatctca
     4321 tctgcctcct ggcttcaacg agtacgactt cgtgccagag tctttcgaca gggacaaaac
     4381 cattgccctg atcatgaaca gctctgggtc taccggcctg cctaagggcg tggccctgcc
     4441 tcatcgcacc gcctgtgtgc gcttctctca cgcccgcgac cctattttcg gcaaccagat
     4501 catccccgac accgctattc tgagcgtggt gccattccac cacggcttcg gcatgttcac
     4561 caccctgggc tacctgattt gcggctttcg ggtggtgctg atgtaccgct tcgaggagga
     4621 gctgttcctg cgcagcctgc aagactacaa aattcagtct gccctgctgg tgccaaccct
     4681 gttcagcttc ttcgctaaga gcaccctgat cgacaagtac gacctgtcta acctgcacga
     4741 gattgcctct ggcggcgccc cactgtctaa ggaggtgggc gaagccgtgg ccaagcgctt
     4801 tcatctgcca ggcatccgcc agggctacgg cctgaccgag acaaccagcg ccattctgat
     4861 taccccagag ggcgacgaca agcctggcgc cgtgggcaag gtggtgccat tcttcgaggc
     4921 caaggtggtg gacctggaca ccggcaagac cctgggagtg aaccagcgcg gcgagctgtg
     4981 tgtgcgcggc cctatgatta tgtccggcta cgtgaataac cctgaggcca caaacgccct
     5041 gatcgacaag gacggctggc tgcactctgg cgacattgcc tactgggacg aggacgagca
     5101 cttcttcatc gtggaccgcc tgaagtctct gatcaagtac aagggctacc aggtggcccc
     5161 agccgagctg gagtctatcc tgctgcagca ccctaacatt ttcgacgccg gagtggccgg
     5221 cctgcccgac gacgatgccg gcgagctgcc tgccgccgtc gtcgtgctgg aacacggcaa
     5281 gaccatgacc gagaaggaga tcgtggacta tgtggccagc caggtgacaa ccgccaagaa
     5341 gctgcgcggc ggagtggtgt tcgtggacga ggtgcccaag ggcctgaccg gcaagctgga
     5401 cgcccgcaag atccgcgaga tcctgatcaa ggctaagaaa ggcggcaaga tcgccgtgta
     5461 ataattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     5521 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5581 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5641 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5701 cctctacaaa tgtggtaaaa tcgataagga tccaggtggc acttttcggg gaaatgtgcg
     5761 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5821 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5881 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5941 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6001 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6061 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6121 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6181 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6241 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6301 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6361 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6421 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6481 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6541 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6601 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6661 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6721 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6781 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6841 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6901 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7021 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7081 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7141 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7201 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7261 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7321 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7381 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7441 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7501 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac
//