| 质粒名称: | pST1374-Lb-Cpf1-NLS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 107680 |
| 存储实验室: | Jia Chen |
|
载体骨架基本信息 |
|
| 载体名称: | Addgene 44758 |
| 完整质粒大小: | 8768 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | LbCpf1 |
| 物种来源: | Lachnospiraceae bacterium |
| 插入大小: | 3711 bp |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知 |
| 3’克隆位点: | 未知 |
| 5’测序引物 | CMV |
| 3’测序引物 | BGH poly reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pST1374-Lb-Cpf1-NLS was a gift from Jia Chen (Addgene plasmid # 107680 ; http://n2t.net/addgene:107680 ; RRID:Addgene_107680) |
| 参考文献部分: | Base editing with a Cpf1-cytidine deaminase fusion. Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X, Yang L, Chen J. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. 10.1038/nbt.4102 PubMed 29553573 |
买家导航
pST1374-Lb-Cpf1-NLS载体质粒基本信息
pST1374-Lb-Cpf1-NLS质粒图谱载体图谱和pST1374-Lb-Cpf1-NLS载体序列质粒序列多克隆位点信息
pST1374-Lb-Cpf1-NLS质粒载体简介
pST1374-Lb-Cpf1-NLS质粒序列载体序列
LOCUS pST1374-Lb-Cpf1-NLS 8768 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses LbCpf1 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pST1374-Lb-Cpf1-NLS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8768)
AUTHORS Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J,
Huang X, Yang L, Chen J
TITLE Base editing with a Cpf1-cytidine deaminase fusion.
JOURNAL Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
PUBMED 29553573
REFERENCE 2 (bases 1 to 8768)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8768
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 907..4590
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/label=LbCpf1
/translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
VKH"
CDS 4597..4617
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4633..4674
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 4684..4701
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(4724..4741)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4730..4954
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5000..5428
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5087..5106)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5297..5318
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5437..5457)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5442..5771
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5622..5757
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5684..5703
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 5819..5866
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 5885..6283
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 6441..6562
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6478..6497)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6532..6551
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6611..6627)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6611..6627)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6624..6646)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6635..6651
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6659..6689)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6704..6725
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6842..6859)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7013..7601)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7093..7112)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7772..8632)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8395..8414
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8633..8737)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgt caaagctgga gaaattcacc aactgttata gcctgtctaa gaccctgcgc
961 ttcaaggcaa tcccagtggg caagacacaa gagaacattg acaacaaacg gctcctggtg
1021 gaggatgaga agagggctga agattacaag ggcgttaaga agctgctgga taggtactat
1081 ctgtcattca tcaacgatgt cctccacagt atcaagctga agaatctgaa caattacatt
1141 tctctgttcc ggaagaagac acggaccgag aaggagaaca aagagctgga gaatctggag
1201 atcaacctga ggaaagaaat agctaaggct ttcaaaggga acgagggtta caagtccctg
1261 ttcaagaaag acattatcga gactattctg cctgagttcc tggacgataa agatgagatc
1321 gccctcgtca attccttcaa tgggtttacc acagccttta ccggcttctt cgacaataga
1381 gagaatatgt tctctgaaga ggccaaatcc actagcatcg cctttcgctg cataaacgag
1441 aacctgacta ggtacatcag caatatggac atctttgaga aagtcgatgc catattcgac
1501 aaacatgagg tgcaggagat taaggagaag atcctgaact cagattacga tgtcgaagat
1561 ttcttcgagg gagagttctt caacttcgtg ctcacacaag agggcattga tgtgtacaat
1621 gcaatcattg gagggttcgt gacagagagt ggcgagaaga taaagggcct gaacgagtat
1681 atcaacctct acaaccagaa aaccaagcag aaactgccta agttcaagcc actgtacaaa
1741 caagtgctct cagataggga aagcctgagc ttctacggtg aagggtatac atcagatgaa
1801 gaagtgctcg aagtgttccg caacaccctc aataagaaca gtgaaatctt ctcttcaatc
1861 aagaagctgg agaaactgtt caagaatttc gatgagtact cctctgccgg aatctttgtg
1921 aagaatggcc ctgcaatatc cactattagc aaagacatct ttggcgagtg gaacgttatc
1981 agggataagt ggaatgccga gtacgatgat attcatctca agaagaaagc cgtggttaca
2041 gagaaatacg aggatgatag acgcaagagc tttaagaaga ttggtagctt ctctctcgaa
2101 cagctgcagg agtacgccga cgctgacctg tcagtcgtgg agaaactcaa ggagatcata
2161 atccagaagg tggatgaaat ctacaaagtg tatggaagct ctgagaaact cttcgatgca
2221 gactttgttc tggagaagag tctgaagaag aacgacgcag tggttgctat catgaaggac
2281 ctgctggatt ctgttaagtc tttcgagaat tacattaagg cattctttgg tgaagggaag
2341 gagacaaata gggacgagag cttctatggc gactttgttc tggcctacga catcctcctc
2401 aaggttgacc acatctatga cgctatacgg aattacgtta cccagaagcc ctatagcaaa
2461 gacaagttca agctgtattt ccagaatcca cagtttatgg gtgggtggga taaagacaaa
2521 gaaacagatt acagggccac tatcctgcgg tacggcagca aatactatct ggctatcatg
2581 gataagaagt acgccaaatg cctccagaag atcgacaagg acgacgtgaa cggtaactac
2641 gagaagatca attacaagct cctgccagga cctaacaaga tgctgcccaa ggtgttcttc
2701 tccaagaaat ggatggccta ctataaccca agcgaggaca ttcagaagat atacaagaat
2761 gggacattca agaagggcga tatgttcaac ctcaacgact gccacaagct gattgatttc
2821 ttcaaggata gcatttctcg ctatcccaag tggtctaatg catacgattt caacttcagc
2881 gagactgaga agtacaaaga catcgctggc ttctaccggg aggtggaaga gcaaggctat
2941 aaggtgtcat tcgaatccgc ttctaagaag gaagtggata agctcgtgga agagggtaag
3001 ctgtacatgt tccagatata caacaaagac ttcagcgata agagccacgg cactccaaac
3061 ctccatacta tgtatttcaa gctgctgttt gacgagaaca accacggaca gattaggctg
3121 tcaggaggcg cagaactctt catgcgcaga gcttcactga agaaggagga actcgttgtc
3181 cacccagcca atagccctat agccaataag aatccagaca atcctaagaa aaccactact
3241 ctgtcttacg atgtgtataa ggataagaga ttctctgaag atcagtacga actgcacata
3301 cccattgcca ttaacaagtg ccctaagaac atcttcaaga ttaacacaga ggttagagtg
3361 ctcctgaaac acgacgataa cccttatgtt ataggcattg atcgcggaga gagaaacctg
3421 ctgtacatcg tcgtggtgga cggcaaaggc aacatcgtgg aacagtacag tctcaatgaa
3481 atcattaaca atttcaacgg aatccgcatt aagaccgact accattctct cctcgacaag
3541 aaggagaaag aaaggttcga agcaagacag aattggacaa gtatagagaa tatcaaagaa
3601 ctgaaggctg ggtacatctc tcaggttgtg cacaagatat gtgagctggt ggagaagtac
3661 gacgctgtta tcgccctcga ggacctgaat agcggcttca agaactccag ggtgaaggtg
3721 gagaagcagg tgtatcagaa gttcgagaag atgctgatcg acaagctcaa ctatatggtg
3781 gacaagaaat ccaatccttg cgctactggt ggagccctga agggctatca aatcaccaat
3841 aagttcgaat ctttcaagtc tatgagcacc cagaatggct tcatcttcta catacccgca
3901 tggctgacat ccaagattga tccctctacc ggatttgtta atctgctcaa gactaagtac
3961 acctctattg ctgactcaaa gaagttcata tcatcatttg accgcatcat gtacgtgcca
4021 gaagaggacc tgttcgagtt tgccctggat tacaagaatt tctctcggac tgacgccgac
4081 tacatcaaga agtggaagct ctactcttat ggtaatcgga ttcgcatatt ccgcaatccc
4141 aagaagaata acgtgttcga ttgggaggaa gtttgcctca ccagcgctta caaggagctg
4201 ttcaataagt atgggattaa ctaccagcag ggcgacataa gagccctgct gtgcgaacaa
4261 tctgataagg cattctattc ctctttcatg gcactgatgt cactgatgct gcaaatgcgc
4321 aattccatca ccggaagaac agacgtggac tttctgatct ctcctgtcaa gaactcagat
4381 ggcatcttct acgattcccg caactatgaa gcacaggaga atgctatcct gcctaagaat
4441 gccgatgcaa atggagccta taacatcgcc agaaaggtcc tctgggccat aggacaattc
4501 aagaaagctg aagatgagaa gctggacaag gtgaagatcg ccatttcaaa caaagagtgg
4561 ctcgaatatg ctcagacctc agtgaagcat ggatcaccca agaagaaacg gaaagtgtga
4621 gggcccttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
4681 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
4741 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
4801 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
4861 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
4921 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
4981 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
5041 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
5101 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
5161 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
5221 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
5281 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
5341 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
5401 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
5461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
5521 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
5581 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
5641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
5701 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
5761 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
5821 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
5881 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
5941 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
6001 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
6061 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
6121 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
6181 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
6241 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg
6301 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
6361 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
6421 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
6481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
6541 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
6601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
6661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
6721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
6781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
6841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
6901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
6961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
7021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
7081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
7141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
7201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
7261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
7321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
7381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
7441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
7501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
7561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
7621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
7681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
7741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
7801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
7861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
7921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
7981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
8041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
8101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
8161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
8221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
8281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
8341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
8401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
8461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
8521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
8581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
8641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
8701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
8761 ctgacgtc
//
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