| 质粒名称: | AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000255 |
| 产品用途: | Expresses NIR-GECO2G, PAmCherry and sfGFP in landing pad cell line |
| 存储实验室: | Peng Zou Lab |
|
载体骨架基本信息 |
|
| 载体名称: | attB |
| 表达系统: | 哺乳动物表达 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | NIR-GECO2G, PAmCherry and sfGFP |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators
Lin C., Liu L., Zou P.
Cell Reports Methods 3, 100544 (2023)
|
AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS载体质粒基本信息
AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS质粒图谱载体图谱和AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS载体序列质粒序列多克隆位点信息
AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS质粒载体简介
AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS质粒序列载体序列
LOCUS AttB-NIR-GECO2G-3NLS-P2A-PAmCherry-NLS-sfGFP-3xNLS 6382 bp DNA circular SYN 03-AUG-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6382)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6382
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..64)
/label=M13-rev
misc_binding complement(70..92)
/label=LacO
rep_origin complement(432..1114)
/direction=LEFT
/label=ColE1 origin
CDS complement(1212..1871)
/label=AmpR
rep_origin complement(2332..2638)
/direction=LEFT
/label=F1 ori
CDS complement(2657..2725)
/label=LacZ alpha
primer_bind 2796..2813
/label=M13-fwd
primer_bind 2812..2839
/label=T7
misc_feature 2910..2930
/label=attB-Left
misc_feature 2931..2951
/label=attB-Right
misc_feature 2971..2979
/label=Feature 13
misc_feature 2974..2979
/label=Kozak sequence
misc_structure 2980..3489
/label=mIFP1-170
misc_structure 3499..3939
/label=Calmodulin
misc_feature 3679..3681
/label=T234I
misc_feature 3754..3756
/label=E259G
misc_structure 3955..4023
/label=RS20
misc_feature 4018..4020
/label=S347G
misc_structure 4036..4464
/label=mIFP178-320
misc_feature 4183..4185
/label=Q402E
misc_feature 4366..4368
/label=F463Y
misc_feature 4411..4413
/label=T478A
CDS 4471..4491
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4495..4515
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4519..4539
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 4540..4605
/label=P2A
/note="Geneious type: motif"
primer_bind 4540..4558
misc_feature 4592..4606
/label=Gibson
primer_bind 4596..4611
misc_structure 4612..5319
/label=PAmCherry1
misc_structure 5326..5346
/label=SV40-NLS
CDS 5356..6069
/codon_start=1
/label=sfGFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
misc_feature 5470..5472
/label=I39N
misc_feature 5668..5670
/label=K105T
misc_feature 5686..5688
/label=V111E
misc_feature 5737..5739
/label=T128I
misc_feature 5851..5856
/label=TV to KI
misc_feature 5968..5970
/label=T205S
CDS 6076..6096
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6100..6120
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6124..6144
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 6154..6382
/label=SV40_term
ORIGIN
1 ggagatcccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
61 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
241 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
1021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
1081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
1141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
1201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
1261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
1321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
1381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
1441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
1501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
1561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
1621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
1681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
1741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
1801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
1861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
1921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
1981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
2041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
2101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
2161 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
2221 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
2281 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
2341 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
2401 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
2461 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
2521 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
2581 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
2641 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
2701 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
2761 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt
2821 aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc gacgatgtag
2881 gtcacggcat tccggactca gcgctcgagc cggcttgtcg acgacggcgg tctccgtcgt
2941 caggatcatc cgcagattat accgcaacta cacgccacca tgtcggtacc gctgactacc
3001 tcagcattcg gccacgcgtt tctggctaac tgtgaacgtg agcagatcca cctggcgggc
3061 tccattcagc cgcacggtat cctgctggct gttaaagagc ctgacaacgt ggtgatccag
3121 gcttctatta acgctgcgga gttcctgaac accaactttg ttgttggccg tccgctgcgt
3181 gacctgggcg gcgatctgcc tttgcagatc ctgccgcacc tgaacggccc gctgcacctg
3241 gctccgatga ccctgcgttg tactgtgggt tctccgccgc gtcgtgtgga ctgtaccatt
3301 catcgaccgt ctaacggcgg cctgatcgta gaactggaac cagcaaccaa ggccactaac
3361 attgcgccgg ctctggtcgg tgcgcttcat cgtatcactt cttcatcctc cctgatgggc
3421 ctgtgtgacg aaaccgcgac tattttccgt gagattactg gcttcgaccg tgtgatggta
3481 atgcgtctcg gcgcgcttga cgatctgact gaagagcaga tcgcagagat taaagaggct
3541 ttctccctat ttgacaagga cggggacggg acgataacaa ccaaggagct ggggacggtg
3601 ttccggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
3661 gatgccgatg gcgacggcat cttcgacttc cctgagttcc tgacgatgat ggcaaggaaa
3721 atgaatgact cagacagtga agaggaaatt agaggcgcgt tccgcgtgtt tgataaggac
3781 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggtgagaag
3841 ttaactgatg aggaggttga tgaaatgatc agggtagcag acaacgatgg ggatggtcag
3901 gtaaactacg aagagtttgt acaaatgatg acagcgaagg gtggcggagg ttctgtagat
3961 tcatcacgtc gtaagtggaa taaggcaggt cacgcagtca gagctatagg tcggctgggc
4021 tcgcgtaggc atgatttgct gtccgaatgt cgtcgtgcgg acctggaagc gttcctgggt
4081 aaccgctacc cggcgtctac tattccgcag atcgctcgtc gcctgtacga acttaaccgt
4141 gttcgcctgc tggtagatgt gaactatact ccggttccgc tagagccgcg catcagcccg
4201 ctgaacggtc gtgatctgga tatgtccctg tcttgcctgc gctctatgtc cccgatccac
4261 cagaaataca tgcaggacat gggcgttggc gcaaccctgg tttgctctct gatggtgtct
4321 ggtcgtctgt ggggtctgat cgtttgccac cactacgaac cgcgctacgt tccgtcccac
4381 attcgcgctg ctggcgaagc gctggcggaa gcctgtgcga accgcatcgc gacgctggag
4441 agctttgcac agtctcagtc caaaaccagg ccgaaaaaga agcgcaaggt tgaccctaag
4501 aagaagcgta aagtcgaccc aaagaagaaa cggaaggtgg gctctggagc caccaacttc
4561 agcctgctga agcaggcagg cgacgtggaa gagaaccctg gccctggatc catggtgagc
4621 aagggcgagg aggataacat ggccatcatt aaggagttca tgcgcttcaa ggtgcacatg
4681 gaggggtccg tgaacggcca cgtgttcgag atcgagggcg agggcgaggg ccgcccctac
4741 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcacctgg
4801 gacatcctga gccctcagtt catgtacggc tccaatgcct acgtgaagca ccccgccgac
4861 atccccgact actttaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaaa
4921 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
4981 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag
5041 aagaccatgg gctgggaggc cctctccgag cggatgtacc ccgaggacgg cgccctgaag
5101 ggcgaggtca agcccagagt gaagctgaag gacggcggcc actacgacgc tgaggtcaag
5161 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caaccgcaag
5221 ctggacatca ccagccacaa cgaggactac accatcgtgg agcagtacga gagagccgag
5281 ggccgccact ccaccggcgg catggacgag ctgtacaagt ctggccctaa gaaaaagcgg
5341 aaagtgggag gcagcatgag caagggcgaa gaactgttta ccggcgtggt gccgattctg
5401 gtggaactgg atggcgatgt gaacggccat aaatttagcg tgcgcggcga aggcgaaggc
5461 gatgcgacca acggcaaact gaccctgaaa tttatttgca ccaccggcaa actaccggtg
5521 ccgtggccga ccctggtgac caccttaacc tatggcgtgc agtgctttag ccgctatccg
5581 gatcatatga aacgccatga tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa
5641 cgcaccatta gctttaaaga tgatggcacc tataaaaccc gcgcggaggt gaaatttgaa
5701 ggcgataccc tggtgaaccg cattgaactg aaaggcatcg attttaaaga agatggcaac
5761 attctggggc ataaactgga atataacttt aacagccata acgtgtatat taccgcggat
5821 aaacagaaaa acggcattaa agcgaacttt aagatccgcc ataacgtgga agatggcagc
5881 gtgcagctgg cggatcatta tcagcagaac accccgattg gcgatggccc ggtgctgctg
5941 ccggataacc attatctgag cacccagtcc gtgctgagca aagatccgaa cgaaaaacgc
6001 gatcacatgg tgctgctgga atttgtgacc gcagcgggca ttacacacgg catggatgaa
6061 ctgtataaga cgcgtccaaa aaagaagaga aaggtagacc cgaagaaaaa gcgtaaggtc
6121 gatcccaaaa agaaacggaa agtgtaagga tcctgatcat aatcagccat accacatttg
6181 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
6241 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
6301 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
6361 ccaaactcat caatgtatct ta
//
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