| 质粒名称: | pAAVLINK-EF1a-LAMA1-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001743 |
| 产品用途: | 5' AAVLINK plasmid for LAMA1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | LAMA1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-LAMA1-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-LAMA1-A载体质粒基本信息
pAAVLINK-EF1a-LAMA1-A质粒图谱载体图谱和pAAVLINK-EF1a-LAMA1-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-LAMA1-A质粒载体简介
pAAVLINK-EF1a-LAMA1-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-LAMA1-A 6901 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6901)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6901
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
misc_feature 724..4041
/label=LAMA1
misc_feature 1271..1276
/label=SDS
misc_feature 1791..1796
/label=SDS
misc_feature 1794..1799
/label=SDS
misc_feature 2876..2881
/label=SDS
intron 4042..4082
/label=Splicing Donor
misc_feature 4086..4119
/label=loxP-JT1571
repeat_region 4164..4304
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4164..4293
/label=AAV2 ITR
rep_origin 4379..4834
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5116..5220
/gene="bla"
/label=AmpR promoter
CDS 5221..6081
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6252..6840
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgcgcg ggggcgtgct cctggtcttg ctgctgtgtg tcgccgcgca gtgccggcag
781 agaggcctgt ttcctgccat tctcaatctt gccagcaatg ctcacatcag caccaatgcc
841 acctgtggcg agaaggggcc ggagatgttc tgcaaacttg tggagcatgt gccaggtcgg
901 cccgtccgaa acccacagtg ccggatctgt gatggcaaca gcgcaaaccc cagagaacgc
961 catccaatat cacatgccat agatggcacc aataactggt ggcaaagtcc cagcattcag
1021 aatgggagag aatatcactg ggtcacaatc actctggact taagacaggt ctttcaagtt
1081 gcatatgtca tcattaaagc tgccaatgcc cctcgacctg gaaactggat tttggagcgt
1141 tctctggatg gcaccacgtt cagcccctgg cagtattatg cagtcagcga ctcagagtgt
1201 ttgtctcgtt acaatataac tccaagacga gggccaccca cctacagggc tgatgatgaa
1261 gtgatctgca cctcctatta ttccagattg gtgccacttg agcatggaga gattcataca
1321 tcactcatca atggcagacc aagcgctgac gatctttcac ccaagttgtt ggaattcact
1381 tctgcacgat atattcgcct tcgcttgcaa cgcattagaa cgctcaatgc agatctcatg
1441 acccttagcc accgggaacc taaagaactg gatcctattg ttaccagacg ctattattat
1501 tcaataaagg acatttctgt tggaggcatg tgtatctgct atggccatgc tagtagctgc
1561 ccatgggatg aaactacaaa gaaactgcag tgtcaatgtg agcataatac ttgcggggag
1621 agctgtaaca ggtgctgtcc tgggtaccat cagcagccct ggaggccggg aaccgtgtcc
1681 tccggcaata catgtgaagc atgtaattgt cacaataaag ccaaagactg ttactatgat
1741 gaaagtgttg caaagcagaa gaaaagtttg aatactgctg gacagttcag aggaggaggg
1801 gtttgcataa attgcttgca gaacaccatg ggaatcaact gtgaaacctg tattgatgga
1861 tattatagac cacacaaagt gtctccttat gaggatgagc cttgccgccc ctgtaattgt
1921 gaccctgtgg ggtccctcag ttctgtctgt attaaggatg acctccattc tgacttacac
1981 aatgggaagc agccaggtca gtgcccatgt aaggaaggtt atacaggaga aaaatgtgat
2041 cgctgccaac ttggctataa ggattacccg acctgtgtct cctgtgggtg caacccagtg
2101 ggcagtgcca gtgatgagcc ctgcacaggg ccctgtgttt gtaaggaaaa cgttgagggg
2161 aaggcctgtg atcgctgcaa gccaggattc tataacttga aggaaaaaaa cccccggggc
2221 tgctccgagt gcttctgctt tggcgtttct gatgtctgca gcagcctctc ttggcctgtt
2281 ggtcaggtaa acagtatgtc cgggtggctg gtcaccgact tgatcagtcc caggaagatc
2341 ccgtctcagc aagatgcact aggcgggcgc catcaggtca gcatcaacaa caccgcggtc
2401 atgcagagac tggctcccaa gtactactgg gcagcccccg aggcctacct tggaaataag
2461 ctgactgcgt ttggcggatt cctgaaatac acggtgtcct acgatattcc ggtagagacg
2521 gtagacagta acctcatgtc gcatgctgac gtcatcatta agggaaacgg actcacttta
2581 agcacacagg ctgagggtct gtcattgcag ccttatgaag agtacctaaa cgtggttaga
2641 cttgtgcctg aaaacttcca agattttcac agcaaaaggc agattgatcg tgaccagctg
2701 atgactgtcc ttgccaatgt gacacatctt ttgatcagag ccaactacaa ttctgcaaaa
2761 atggctcttt acaggttgga gtccgtctct ctggacatag ccagctctaa tgccatcgac
2821 ctggtggtgg ccgctgatgt ggagcactgt gaatgtccgc aaggctacac agggacctcc
2881 tgtgagtcgt gcctctctgg ctattaccgc gtggatggaa tactctttgg aggaatttgt
2941 caaccctgtg aatgccacgg ccatgcagct gagtgtaatg ttcacggcgt ttgcattgcg
3001 tgtgcgcaca acaccaccgg cgtccactgt gagcagtgct tgcccggctt ctacggggag
3061 ccttcccgag ggacacctgg ggactgccag ccctgcgcct gccctctcac catagcctcc
3121 aacaatttca gccccacctg ccacctcaat gatggagatg aagtggtctg tgactggtgt
3181 gccccgggct actcaggagc ttggtgtgag agatgtgcag atggttacta tggaaaccca
3241 acagtgcctg gcgaatcttg tgttccctgt gactgcagcg gcaacgtgga cccctcggag
3301 gctggtcact gtgactcagt caccggggag tgcctgaagt gcctggggaa cacagatggc
3361 gcccactgtg aaaggtgtgc tgacgggttc tatggggacg ctgtgacagc caagaactgc
3421 cgcgcctgtg aatgccatgt gaaaggctcc cattctgccg tgtgccatct tgagaccggg
3481 ctctgtgact gcaaaccaaa cgtgactgga cagcagtgtg accagtgctt gcatggctat
3541 tatgggctgg actcaggcca tggctgccgg ccctgcaact gcagcgtggc aggctccgtg
3601 tcagatggct gcacggatga aggccagtgt cactgtgtcc caggtgtggc agggaaaagg
3661 tgtgacaggt gtgcccatgg cttctacgcc taccaggatg gtagctgtac accctgtgac
3721 tgcccacaca ctcagaatac ctgcgaccca gaaactggag agtgtgtctg cccccctcac
3781 acacagggtg tgaagtgtga agaatgtgag gatgggcact ggggctacga tgcggaggtg
3841 gggtgccagg cctgcaattg cagtctcgtg gggtcgactc atcatcggtg cgatgtggtc
3901 accggccatt gccagtgcaa gtcaaaattt ggtggccggg cctgcgatca gtgttccttg
3961 ggttacagag actttcccga ctgtgttccc tgtgactgtg acctgagggg gacgtcgggg
4021 gacgcctgca acctggagca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
4081 taagctatta ttcgtatagc atacattata cgaagttatt aagttatgga gaccggtgtg
4141 gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
4201 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
4261 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
4321 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4381 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4441 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4501 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4561 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4621 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4681 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
4741 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4801 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4861 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4921 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4981 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
5041 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
5101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
5221 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5281 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
5341 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5401 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5461 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5521 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5581 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5641 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5701 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5761 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5821 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5881 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5941 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
6001 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
6061 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6121 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6361 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
6421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6601 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6901 t
//
