| 质粒名称: | pYK704 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000840 |
| 产品用途: | Mammalian PhCMV-driven constitutive AG-LplA expression vector (PhCMV-AG-LplA-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | AG-LplA |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK704
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK704载体质粒基本信息
pYK704质粒图谱载体图谱和pYK704载体序列质粒序列多克隆位点信息
pYK704质粒载体简介
pYK704质粒序列载体序列
LOCUS pYK704 7159 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7159)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7159
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..928
/label=oYK255_AG_NS3a
primer_bind 881..925
/label=oYK36-AG-Link_s-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 887..904
/label=oYK663_Nhe_F
CDS 906
/codon_start=1
/label=5xGS Linker
/translation=""
primer_bind 907..926
/label=oYK41-44 AG-LplA-F
primer_bind 907..926
/label=oYK45-46 AG-LplA-F
misc_feature 910..1584
/label=AG
primer_bind 1358..1368
/label=oSCL1104
misc_feature 1585..1635
/label=Short_Linker
primer_bind complement(1611..1655)
/label=oYK36-AG-Link_s-R
primer_bind complement(1611..1635)
/label=oYK33-35-PB1-AG-R
misc_feature 1636..2657
/label=LPLA
/note="LplA"
primer_bind 1636..1654
/label=oYK36-37-LplA-F
primer_bind 1912..1934
/label=oYK08_lpl91-F
primer_bind 1974..1990
/label=oYK09_lpl112-F
primer_bind 2020..2035
/label=oYK10_lpl127-F
primer_bind 2065..2083
/label=oYK11_lpl142-F
primer_bind 2101..2119
/label=oYK12_lpl154-F
primer_bind 2164..2186
/label=oYK13_lpl175-F
primer_bind 2212..2230
/label=oYK14_lpl191-F
primer_bind 2275..2290
/label=oYK15-lpl212-F
primer_bind 2301..2320
/label=oYK16_lpl221-F
primer_bind 2359..2380
/label=oYK17_lpl240-F
primer_bind 2383..2401
/label=oYK18_lpl248-F
primer_bind 2419..2433
/label=oYK19_lpl260-F
/note="Maybe mismatch"
primer_bind 2455..2473
/label=oYK20_lpl272-F
primer_bind 2486..2502
/label=oYK21_lpl283-F
primer_bind 2533..2550
/label=oYK22_lpl298-F
/note="DNA may less than 100 bp"
primer_bind complement(2633..2652)
primer_bind complement(2635..2652)
/label=oYK404_LplA_R
primer_bind complement(2637..2652)
/label=oYK228_lplA_R
primer_bind complement(2637..2652)
/label=oYK341_lplA_R
primer_bind complement(2637..2652)
/label=oYK372_LplA_R
primer_bind complement(2642..2662)
/label=oYK01-1-R
primer_bind complement(2652..2676)
/label=oYK08-22-LplApart-R
primer_bind complement(2655..2676)
/label=oYK36-37-LplA-R
primer_bind complement(2657..2679)
/label=oYK57-MCP-mCherry-R
terminator 2753..2980
/label=bGH PA
misc_feature 3026..3454
/label=f1 origin
misc_feature 3459..3802
/label=SV40 early promoter and origin
misc_feature 3648..3725
/label=SV40 ORI
CDS 3867..4658
/label=Kan/neoR
misc_feature 4832..4962
/label=SV40 early polyadenylation signal
primer_bind complement(4998..5018)
/label=M13-rev
misc_binding complement(5024..5046)
/label=LacO
promoter complement(5051..5080)
/label=lac
misc_feature complement(5345..6018)
/label=pUC origin
CDS complement(6166..6825)
/label=AmpR
promoter complement(7065..7093)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg tgagcgtgat caagcccgag atgaagatca agctgtgcat gaggggcacc
961 gtgaacggcc acaacttcgt gatcgagggc gagggcaagg gcaaccccta cgagggcacc
1021 cagatcctgg acctgaacgt gaccgagggc gcccccctgc ccttcgccta cgacatcctg
1081 accaccgtgt tccagtacgg caacagggcc ttcaccaagt accccgccga catccaggac
1141 tacttcaagc agaccttccc cgagggctac cactgggaga ggagcatgac ctacgaggac
1201 cagggcatct gcaccgccac cagcaacatc agcatgaggg gcgactgctt cttctacgac
1261 atcaggttcg acggcgtgaa cttccccccc aacggccccg tgatgcagaa gaagaccctg
1321 aagtgggagc ccagcaccga gaagatgtac gtgagggacg gcgtgctgaa gggcgacgtg
1381 aacatggccc tgctgctgga gggcggcggc cactacaggt gcgacttcaa gaccacctac
1441 aaggccaaga aggacgtgag gctgcccgac taccacttcg tggaccacag gatcgagatc
1501 ctgaagcacg acaaggacta caacaaggtg aagctgtacg agaacgccgt ggccaggtac
1561 agcatgctgc ccagccaggc caagggtacc ggaactgcag cagagaattc gggaaactcg
1621 agaacaaagc ttgaaatggg ctccacatta cgcctgctca tctctgactc ttacgacccg
1681 tggtttaacc tggcggtgga agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt
1741 ctgtttctct ggcgcaatgc cgacacggta gtaattggtc gcgcgcagaa cccgtggaaa
1801 gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt
1861 ggcggcgcgg tgttccacga tctcggcaat acctgcttta cctttatggc tggcaagccg
1921 gagtacgata aaactatctc cacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc
1981 agcgccgaag cgtccggacg taacgatctg gtggtgaaaa ccgtcgaagg cgaccgcaaa
2041 gtctcaggct cggcctatcg cgaaaccaaa gatcgcggct tccaccacgg caccttgcta
2101 ctcaatgccg acctcagccg cctggcaaac tatctcaatc cggataaaaa gaaactggcg
2161 gcgaaaggca ttacgtcggt acgttcccgc gtgaccaacc tcaccgagct gttgccgggg
2221 atcacccatg agcaggtttg cgaggccata accgaggcct ttttcgccca ttatggcgag
2281 cgcgtggaag cggaaatcat ctccccgaac aaaacgccag acttgccaaa cttcgccgaa
2341 acctttgccc gccagagtag ctgggaatgg aacttcggtc aggctccggc attctcgcat
2401 ctgctggatg aacgctttac ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc
2461 catatcaccc gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct ggaagccctc
2521 gccggacgac tgcaaggctg cctgtaccgc gcagatatgc tgcaacagga gtgcgaagcg
2581 ctgttggttg acttcccgga acaggaaaaa gagctacggg agttatcggc atggatggcg
2641 ggggctgtaa ggtaggggat ccactagtcc agtgtggtgg aattctgcag atatccagca
2701 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
2761 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
2821 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
2881 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
2941 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
3001 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
3061 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
3121 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3181 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3241 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3301 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
3361 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
3421 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
3481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
3541 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3601 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
3661 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
3721 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
3781 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
3841 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
3901 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
3961 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
4021 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
4081 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
4141 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
4201 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
4261 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
4321 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
4381 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
4441 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
4501 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
4561 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
4621 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
4681 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
4741 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
4801 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
4861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
4921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
4981 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
5041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
5101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
5161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
5221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
5281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
5341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
5401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
5461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
5521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
5581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
5641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
5701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
5761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
5821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
5881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
5941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
6001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
6061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
6121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
6181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
6241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
6301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
6361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
6421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
6481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
6541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
6601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
6661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
6721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
6781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
6841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
6901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
6961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
7021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
7081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
7141 aaaagtgcca cctgacgtc
//
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