| 质粒名称: | pAAV-GfaABC1D_hCRAFi-CCR2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001181 |
| 产品用途: | Detecting CCL2 |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | GfaABC1D |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 or DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | hCRAFi-CCR2 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Genetically encoded biosensor for monitoring spatiotemporal dynamics of CCR2 ligands in culture and in vivo.
Xiao X., Wang C., Guo X., Xi F., Qian Q., Liang G., Chen M., Sun X., Szabo B., Jing M., Piatkevich K.D.
Nat. Methods 2025 1–11 (2025)
|
pAAV-GfaABC1D_hCRAFi-CCR2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-GfaABC1D_hCRAFi-CCR2载体质粒基本信息
pAAV-GfaABC1D_hCRAFi-CCR2质粒图谱载体图谱和pAAV-GfaABC1D_hCRAFi-CCR2载体序列质粒序列多克隆位点信息
pAAV-GfaABC1D_hCRAFi-CCR2质粒载体简介
pAAV-GfaABC1D_hCRAFi-CCR2质粒序列载体序列
LOCUS pAAV-GfaABC1D_hCRAFi-CCR2 7153 bp DNA circular SYN 25-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7153)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7153
/mol_type="other DNA"
/organism="synthetic DNA construct"
intron 995..1127
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 1127..1150
/label=gfabc_seq_Fw
primer_bind 1168..1226
/label=gfa_NheI_hR2_Fw
promoter 1172..1190
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 1207..1212
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1213..3036
/codon_start=1
/product="Isoform B"
/label=hCCR2B
/translation="MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLL
PPLYSLVFIFGFVGNMLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAAN
EWVFGNAMCKLFTGLYHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVI
TWLVAVFASVPGIIFTKCQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICY
SGILKTLLRCRNGLEEGGNVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNIDKKRHRAVRVIFTIMIVYFLFWTPYNIVILLNTFQ
EFFGLSNCESTSQLDQATQVTETLGMTHCCINPIIYAFVGEKFRRYLSVFFRKHITKRF
CKQCPVFYRETVDGVTSTNTPSTGEQEVSAGL"
primer_bind 1213..1235
/label=R2_Fw
primer_bind complement(1339..1384)
/label=Y49A_Rv_new
primer_bind 1360..1384
/label=Y49A_new_Fw
primer_bind 1471..1491
/label=sequencing primer
primer_bind complement(1548..1593)
/label=Y120A_Rv_new
primer_bind 1573..1593
/label=Y120A_new_Fw
primer_bind 1834..1851
/label=NEW SEQUENCING PRIMER
primer_bind complement(1896..1938)
/label=R2_LEGG_Rv
primer_bind complement(1902..1959)
/label=R2-3_L1_IO_2aa_Rv
primer_bind complement(1904..1959)
/label=R2-3_L1_IO_3aa_Rv
primer_bind complement(1904..1915)
/label=NT_SV_GFP_Erro_RV
primer_bind 1918..1959
/label=LEGG_GFP_Fw
CDS 1921..1932
/codon_start=1
/product="Isoform B"
/label=hCCR2B
/translation="EEGG"
CDS 1933..2655
/codon_start=1
/product="improved fluorescent protein-based calcium sensor
(Chen et al., 2013)"
/label=cpGFP
/translation="NVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
KGIDFKEDGNILGHKLEYN"
primer_bind 3002..3058
/label=HCCR2b citrine_KGCX3 Fw
primer_bind complement(3002..3058)
/label=引物 HCCR2b citrine_KGCX3 Rv
CDS 3052..3111
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
primer_bind complement(3084..3111)
/label=KGC_GG_Rv
CDS 3112..3171
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
primer_bind complement(3144..3171)
/label=KGC_GG_Rv
CDS 3172..3231
/codon_start=1
/label=KGC
/translation="KSRITSEGEYIPLDQIDINV"
primer_bind complement(3204..3237)
/label=KGC_GG_Rv
primer_bind complement(3228..3286)
/label=gfap_ER2_EcoRV_Rv
primer_bind complement(3228..3286)
/label=gfabc_KGC_ER2_EcoRV_Rv
primer_bind complement(3232..3261)
/label=GGER2_NotI_Rv
CDS 3238..3258
/codon_start=1
/label=ER2
/translation="FCYENEV"
misc_feature 3280..3868
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3751..3762)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind 3816..3827
/label=Y49A_new_Fw
polyA_signal 3900..4376
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
repeat_region 4416..4556
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4631..5086
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5368..5472
/gene="bla"
/label=AmpR promoter
CDS 5473..6333
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6504..7092
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgtagt taatgattaa cccgccatgc tacttatcta
181 cgtagccatg ctctaggaag atctaacata tcctggtgtg gagtagggga cgctgctctg
241 acagaggctc gggggcctga gctggctctg tgagctgggg aggaggcaga cagccaggcc
301 ttgtctgcaa gcagacctgg cagcattggg ctggccgccc cccagggcct cctcttcatg
361 cccagtgaat gactcacctt ggcacagaca caatgttcgg ggtgggcaca gtgcctgctt
421 cccgccgcac cccagccccc ctcaaatgcc ttccgagaag cccattgagc agggggcttg
481 cattgcaccc cagcctgaca gcctggcatc ttgggataaa agcagcacag ccccctaggg
541 gctgcccttg ctgtgtggcg ccaccggcgg tggagaacaa ggctctattc agcctgtgcc
601 caggaaaggg gatcagggga tgcccaggca tggacagtgg gtggcagggg gggagaggag
661 ggctgtctgc ttcccagaag tccaaggaca caaatgggtg aggggagagc tctccccata
721 gctgggctgc ggcccaaccc caccccctca ggctatgcca gggggtgttg ccaggggcac
781 ccgggcatcg ccagtctagc ccactccttc ataaagccct cgcatcccag gagcgagcag
841 agccagagca ggttggagag gagacgcatc acctccgctg ctcgcaagct ttattgcggt
901 agtttatcac agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt
961 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
1021 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
1081 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
1141 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagcctcga
1201 gaattcgcca ccatgctgag caccagcaga agcagattca tcagaaacac caacgagagc
1261 ggcgaggagg tgaccacctt cttcgactac gactacggcg ccccctgcca caagttcgac
1321 gtgaagcaga tcggcgccca gctgctgccc cccctgtaca gcctggtgtt catcttcggc
1381 ttcgtgggca acatgctggt ggtgctgatc ctgatcaact gcaagaagct gaagtgcctg
1441 accgacatct acctgctgaa cctggccatc agcgacctgc tgttcctgat caccctgccc
1501 ctgtgggccc acagcgccgc caacgagtgg gtgttcggca acgccatgtg caagctgttc
1561 accggcctgt accacatcgg ctacttcggc ggcatcttct tcatcatcct gctgaccatc
1621 gacagatacc tggccatcgt gcacgccgtg ttcgccctga aggccagaac cgtgaccttc
1681 ggcgtggtga ccagcgtgat cacctggctg gtggccgtgt tcgccagcgt gcccggcatc
1741 atcttcacca agtgccagaa ggaggacagc gtgtacgtgt gcggccccta cttccccaga
1801 ggctggaaca acttccacac catcatgaga aacatcctgg gcctggtgct gcccctgctg
1861 atcatggtga tctgctacag cggcatcctg aagaccctgc tgagatgcag aaacggcctg
1921 gaggagggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
1981 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
2041 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtgcag
2101 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
2161 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
2221 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
2281 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
2341 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
2401 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
2461 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
2521 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
2581 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
2641 aagctggagt acaacatcga caagaagaga cacagagccg tgagagtgat cttcaccatc
2701 atgatcgtgt acttcctgtt ctggaccccc tacaacatcg tgatcctgct gaacaccttc
2761 caggagttct tcggcctgag caactgcgag agcaccagcc agctggacca ggccacccag
2821 gtgaccgaga ccctgggcat gacccactgc tgcatcaacc ccatcatcta cgccttcgtg
2881 ggcgagaagt tcagaagata cctgagcgtg ttcttcagaa agcacatcac caagagattc
2941 tgcaagcagt gccccgtgtt ctacagagag accgtggacg gcgtgaccag caccaacacc
3001 cccagcaccg gcgagcagga ggtgagcgcc ggcctgatgg acgagctgta caagagtaga
3061 atcacaagcg aaggcgagta catccccctg gatcaaatag acataaatgt aaagagtaga
3121 atcacaagcg aaggcgagta catccccctg gatcaaatag acataaatgt aaagagtaga
3181 atcacaagcg aaggcgagta catccccctg gatcaaatag acataaatgt aggtggattt
3241 tgttatgaga atgaagtgta agatatcaag cttatcgata atcaacctct ggattacaaa
3301 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
3361 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
3421 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
3481 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
3541 tgtcagctcc tttccggaac tttcgctttc cccctcccta ttgccacggc ggaactcatc
3601 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
3661 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
3721 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
3781 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
3841 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
3901 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
3961 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
4021 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
4081 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
4141 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
4201 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
4261 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
4321 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
4381 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
4441 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
4501 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
4561 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
4621 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4681 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
4741 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4801 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
4861 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4921 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
4981 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
5041 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
5101 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
5161 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
5221 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
5281 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
5341 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
5401 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
5461 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
5521 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
5581 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
5641 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
5701 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
5761 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
5821 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
5881 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
5941 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
6001 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
6061 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
6121 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
6181 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
6241 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
6301 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
6361 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
6421 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
6481 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
6541 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
6601 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
6661 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6721 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6781 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6841 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6901 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6961 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
7021 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
7081 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
7141 tttgctcaca tgt
//
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pAAV-GfaABC1D_hCRAFi-CCR2质粒载体应用举例
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