pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e载体质粒基本信息

质粒名称: pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e
出品公司: Wekwikgene
目录编号: 0001903
产品用途: 3' AAVLINK plasmid for ABE8e expression
存储实验室: Zhonghua Lu Lab

载体骨架基本信息
载体名称: pAAV-MCS2
表达系统: 腺病毒
启动子: SCP1
拷贝数: 高拷贝
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: ABE8e
物种来源: 未知
密码子优化: 未优化

文献引用方法
参考文献部分:
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e载体序列质粒序列多克隆位点信息

pAAVLINKv2-SCP1-Cre-UDeg3a-3-ABE8e载体图谱

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e质粒载体简介

pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e质粒序列载体序列

LOCUS      pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ABE8e               6612 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     repeat_region   47..57
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    156..236
                     /label=SCP1
     CDS             248..1276
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1292..1381
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1306..1311
                     /label=SDS
     misc_feature    1309..1314
                     /label=SDS
     intron          1385..1425
                     /label=Splicing Donor
     protein_bind    1432..1465
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1466..1557
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1558..3345
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=ABE8e
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="GQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQ
                     NGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVV
                     KKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQIL
                     DSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVG
                     TALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLA
                     NGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILP
                     KRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMER
                     SSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPS
                     KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDK
                     VLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLI
                     HQSITGLYETRIDLSQLGGD"
     CDS             1948..3345
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDEND
                     KLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLES
                     EFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIET
                     NGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKK
                     DWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLE
                     AKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHY
                     EKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP
                     IREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
                     DLSQLGGD"
     CDS             3388..3408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3412..3843
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3556..3561
                     /label=SDS
     repeat_region   3886..4015
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     rep_origin      complement(4077..4665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4836..5696)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5697..5801)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6083..6538)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtact tatataaggg ggtgggggcg
      181 cgttcgtcct cagtcgcgat cgaacactcg agccgagcag acgtgcctac ggaccgcgta
      241 cgccaccatg tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc
      301 aacgagtgat gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc
      361 tgagcatacc tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt
      421 gaataaccgg aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct
      481 tcaggcgcgc ggtctggcag taaaaaccat ccagcaacat ttgggccagc taaacatgct
      541 tcatcgtcgg tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg
      601 gcggatccga aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga
      661 acgcactgat ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat
      721 acgtaatctg gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc
      781 caggatcagg gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg
      841 cagaacgaaa acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac
      901 taaactggtc gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct
      961 gttttgccgg gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac
     1021 tcgcgccctg gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga
     1081 ctctggtcag agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga
     1141 tatggcccgc gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt
     1201 aaatattgta atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct
     1261 gctggaagat ggcgatggag gtggatctgg tctgctgctg aaactcctcc tcctcggtgg
     1321 tggttctggt cctcgtcccc gtggtactcg tggtaaaggt cgccgtattc actacaaagg
     1381 ataagtaagt atcaaggtta caagacaggt ttaaggagac caataactag tataacttcg
     1441 tataggatac tttatagcaa tttatgaaac tgggcttgtc gagacagaga agactcttgc
     1501 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggga
     1561 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     1621 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     1681 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     1741 tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     1801 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     1861 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     1921 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     1981 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     2041 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     2101 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     2161 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     2221 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     2281 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     2341 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     2401 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     2461 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     2521 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     2581 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     2641 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     2701 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     2761 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     2821 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     2881 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     2941 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     3001 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     3061 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     3121 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     3181 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     3241 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     3301 ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactctgg cggctcaaaa
     3361 agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta actcgagata
     3421 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3481 cttttacgct atgtggatac gctgctttaa tgcctttgca tcatgctatt gcttcccgta
     3541 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
     3601 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3661 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
     3721 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3781 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
     3841 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     3901 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     3961 tcgcccgacg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggacatg
     4021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4441 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4501 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4561 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4621 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4681 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4741 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4801 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4861 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4921 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4981 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5041 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5101 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5161 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5221 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5281 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5341 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5401 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5461 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5521 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5581 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5641 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5701 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5761 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5821 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5881 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5941 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     6001 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     6061 attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa attcgcgtta
     6121 aatttttgtt aaatcagctc attttttaac caatagaccg aaatcggcaa aatcccttat
     6181 aaatcaaaag aatagcccga gatagagttg agtgttgttc cagtttggaa caagagtcca
     6241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     6301 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     6361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgagcgtg
     6421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta aggcgctggc aagtgtagcg
     6481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac
     6541 tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6601 gcatcaggcg cc
//

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