pYK648

LOCUS       pYK648            6849 bp DNA     circular SYN 11-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..946
                     /label=oYF0019
     primer_bind     881..918
                     /label=oYK421_GNCR_F
     primer_bind     881..902
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     881..902
                     /label=oYK232_tTA-F
     primer_bind     881..900
                     /label=oYK248_NLStetR_F
     primer_bind     910..952
                     /label=oYF005
     primer_bind     916..937
                     /label=oYK481_GNCR_F
     primer_bind     916..937
                     /label=oYK483_LCK1_F
     primer_bind     916..936
                     /label=oYK490_P2A_GNCR1_F
     CDS             919..1617
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     primer_bind     919..952
                     /label=oYF0011
     primer_bind     919..943
                     /label=oLZ146 GNCR1-N F
     primer_bind     919..943
                     /label=oSCL762
     primer_bind     1585..1597
                     /label=oYK03-02-F
     primer_bind     1585..1596
                     /label=oYK31-mCherry-NS3a-F
     primer_bind     complement(1596..1653)
                     /label=oYF0020
     primer_bind     complement(1596..1647)
                     /label=oYF006
     primer_bind     complement(1596..1618)
                     /label=oYF0012
     primer_bind     complement(1605..1624)
                     /label=oYK465_GNCR1_R
     primer_bind     1615..1637
                     /label=oSCL1129
     primer_bind     1615..1635
                     /label=oLZ147 NS3a F
     primer_bind     1615..1634
                     /label=oYF0033
     primer_bind     complement(1616..1637)
                     /label=oYK248_TetR-R
     primer_bind     1617..1637
                     /label=oLZ048 NSP3-F
     primer_bind     1617..1634
                     /label=oYK419_GS_F
     primer_bind     1618..1647
                     /label=oSCL388
     primer_bind     1618..1633
                     /label=oYK28-mCherry-NS3a-F
     primer_bind     complement(1619..1667)
                     /label=oLZ145 DNCR2-GNCR1 R
     primer_bind     complement(1625..1643)
                     /label=Oscl18-4
     primer_bind     complement(1628..1684)
                     /label=oSCL1119
     primer_bind     complement(1628..1647)
                     /label=oSCL075
     primer_bind     complement(1628..1647)
                     /label=oSCL075a
     primer_bind     complement(1628..1647)
                     /label=oSCL075`b
     primer_bind     complement(1631..1647)
                     /label=oYK472_NLS_R
     primer_bind     1632..1652
                     /label=oLZ146 GNCR1-N F
     primer_bind     complement(1632..1648)
                     /label=oYK92_L7Ae_R
     primer_bind     complement(1634..1662)
                     /label=oYK489_GNCR1_R
     misc_feature    1634..1653
                     /label=linker
     primer_bind     complement(1636..1648)
                     /label=oYK75_NLS_mcp_R
     primer_bind     complement(1645..1668)
                     /label=oYK421_GNCR3_R
     primer_bind     1649..1686
                     /label=oYK648_NLS_F
     misc_feature    1654..1668
                     /label=NSP3
     primer_bind     1668..1754
                     /label=oYK248_NLStetR_F
     CDS             1668..1725
                     /codon_start=1
                     /label=NLS
                     /translation="PKKKRKVEASASPKKKRKV"
     primer_bind     1693..1760
                     /label=oYK232_tTA-F
     primer_bind     1706..1725
                     /label=oYK419_GS_F
     CDS             1726..2343
                     /label=Translation 2573-5638
     misc_feature    1726..2343
                     /label=tetR
     primer_bind     complement(2325..2370)
                     /label=oYK278_tetR_R
     primer_bind     complement(2326..2343)
                     /label=oYK248_TetR-R
     primer_bind     complement(2343..2369)
                     /label=oYK472_NLS_R
     primer_bind     complement(2347..2369)
                     /label=oYK57-MCP-mCherry-R
     terminator      2443..2670
                     /label=bGH PA
     misc_feature    2716..3144
                     /label=f1 origin
     misc_feature    3149..3492
                     /label=SV40 early promoter and origin
     misc_feature    3338..3415
                     /label=SV40 ORI
     CDS             3557..4348
                     /label=Kan/neoR
     misc_feature    4522..4652
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(4688..4708)
                     /label=M13-rev
     misc_binding    complement(4714..4736)
                     /label=LacO
     promoter        complement(4741..4770)
                     /label=lac
     misc_feature    complement(5035..5708)
                     /label=pUC origin
     CDS             complement(5856..6515)
                     /label=AmpR
     promoter        complement(6755..6783)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
      961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
     1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
     1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
     1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
     1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
     1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
     1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
     1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
     1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
     1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
     1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
     1621 agcggaagtg gcggaggagg cgacgtcgct agcatgctca agatggagcc caagaagaag
     1681 cgcaaggtgg aagctagcgc ttccccgaag aaaaagcgga aagtcatgtc tagactggac
     1741 aagagcaaag tcataaactc tgctctggaa ttactcaatg aagtcggtat cgaaggcctg
     1801 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     1861 aagaacaagc gggccctgct cgatgccctg gcaatcgaga tgctggacag gcatcatacc
     1921 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     1981 ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     2041 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     2101 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     2161 ttggaggatc aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     2221 atgcccccac ttctgagaca agcaattgag ctgttcgacc atcagggagc cgaacctgcc
     2281 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     2341 ggctaaggat ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc
     2401 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2461 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2521 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2581 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2641 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     2701 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2761 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2821 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     2881 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     2941 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3001 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3061 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3121 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     3181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3241 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3301 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3421 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3481 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     3541 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3601 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3661 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3721 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3781 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3841 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3901 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3961 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4021 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4081 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4141 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4201 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4261 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     4321 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     4381 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4441 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4501 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     4561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4621 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     4681 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4741 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4801 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4861 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4921 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4981 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5041 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5101 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5161 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5221 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5281 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5341 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5401 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5461 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5521 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5581 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5641 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5701 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5761 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5821 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5881 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5941 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6001 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6061 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6121 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6181 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6241 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6301 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6361 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6421 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6481 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6541 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6601 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6661 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6721 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6781 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6841 cctgacgtc
//