| 质粒名称: | pAAVLINK-EF1a-HA-5'-MED12L |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001707 |
| 产品用途: | 5' AAVLINK plasmid for MED12L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MED12L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-MED12L
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-MED12L载体质粒基本信息
pAAVLINK-EF1a-HA-5'-MED12L质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-MED12L载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-MED12L质粒载体简介
pAAVLINK-EF1a-HA-5'-MED12L质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-MED12L 6432 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6432)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6432
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3572
/codon_start=1
/label=MED12L
/translation="MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGF
NNQPAFTGDEHGSARNIVINPSKIGAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWL
VTARSQSAIHSWFSDLAGNKPLSILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMT
CAYYSAISEAKIKKRQAPDPNLEWTQISTRYLREQLAKISDFYHMASSTGDGPVPVPPE
VEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYS
DEFVQSAYLSRRLAYFCARRLSLLLSDSPNLLAAHSPHMMIGPNNSSIGAPSPGPPGPG
MSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNENKSANPGSPLDL
LPVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISR
VLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSC
KRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSSSLAGSSLPVFQNVLLRF
LDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVTASTRP
RSPVGENADEHYSKDHDVKMEEQSIMAHMGIDSGTTNIFDEVDKSDFKTDFGSEFPIFS
PMPGESCENANTSLGRRMSVNCEKLVKREKPRELIFPSNYDLLRHLQYATHFPIPLDES
SSHECNQRTILLYGVGKERDEARHQLKKITKDILKILNKKSTTETGVGDEGQKARKNKQ
ETFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDL
MEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLI
LNPDQTAQVFE"
misc_feature 843..848
/label=SDS
misc_feature 1681..1686
/label=SDS
misc_feature 2953..2958
/label=SDS
intron 3573..3613
/label=Splicing Donor
misc_feature 3617..3650
/label=lox2272-JT1571
repeat_region 3695..3835
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3695..3824
/label=AAV2 ITR
rep_origin 3910..4365
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4647..4751
/gene="bla"
/label=AmpR promoter
CDS 4752..5612
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5783..6371
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgctct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccgccttc gggcttctca gctatgagca
781 gagaccgctg aagcgccccc ggctcgggcc gcccgacgtc tacccacagg accccaagca
841 gaaggaggat gaacttactg ctgtgaatgt aaagcaaggc ttcaataatc agccagcctt
901 cactggagat gaacatggct cagccagaaa tattgtaatt aacccatcaa agattggagc
961 ttattttagc agcatattag ctgagaaact gaagcttaac actttccagg acacgggaaa
1021 gaagaaacca caagttaatg ctaaagataa ttattggctg gttactgctc gatcccagag
1081 tgcaattcat agttggtttt ctgacttagc aggaaataag ccactttcta ttttggcaaa
1141 aaaggttcct atccttagta aaaaagagga tgtttttgca tatttagcta aatattctgt
1201 gccaatggtt cgagcaacgt ggctgatcaa gatgacttgt gcctattatt ctgctatatc
1261 tgaagctaaa attaagaaac gtcaggctcc tgatccgaat ttggagtgga cacagatatc
1321 taccagatat cttcgagagc agttggccaa gatttctgac ttttaccaca tggcctccag
1381 cacgggcgat ggccctgtcc ctgtgccacc agaggtggag caagccatga agcaatggga
1441 atacaacgaa aagctagcat ttcacatgtt ccaggaagga atgttagaaa aacacgaata
1501 tttgacatgg atcctggatg ttttagaaaa gatcagacca atggatgatg atcttcttaa
1561 actcttgcta ccactaatgc tgcagtattc agatgagttt gttcagtcgg cctacctgtc
1621 tcgtcgtctt gcctactttt gtgcccggcg tctttccttg ctgctgagcg atagccccaa
1681 cctccttgct gcccactcac cccacatgat gataggacca aacaactcga gtatcggggc
1741 ccccagccct ggcccccccg gccctggcat gagccccgtg cagctggcct tctcagattt
1801 tctttcctgt gcacagcatg gtcccctggt ttatggactt agttgtatgt tgcagactgt
1861 cactctctgt tgcccaagtg ccttggtgtg gaattattcc acaaatgaaa ataagagcgc
1921 aaacccaggc tcacccctgg atctgctgcc ggtggccccg tccagcctcc ccatgccggg
1981 tgggaacacg gctttcaatc agcaggttcg ggcaaggatt tatgaagtag aacaacagat
2041 aaaacaaaga ggccgtgcag tggaagttcg gtggtcattt gacaagtgcc aagaatccac
2101 agcaggggtg actattagtc gggttttgca cacgttggaa gttttggatc gtcactgttt
2161 tgaccgaact gattccagca attccatgga gacactttat cataagattt tctgggcaaa
2221 ccaaaacaaa gataaccaag aggttgcgcc caacgatgaa gctgtggtga cgctgttatg
2281 tgaatgggcc gtgagctgca aacggtctgg caagcacagg gccatggctg tggcaaaact
2341 cctggagaag aggcaagcag aaattgaggc agagagatgt ggtgaatcag aagtcttaga
2401 tgagaaggag tctatttctt catcctctct tgctggatcc agtttgcctg ttttccagaa
2461 tgtgctgtta aggtttttag atacacaggc cccctctttg tcggacccaa acagtgaatg
2521 tgaaaaggtg gaatttgtga acctggtgct gctcttctgc gagttcatcc gccatgatgt
2581 cttctcccat gacgcataca tgtgtaccct catatctcga ggagatttgt cagtcactgc
2641 ctcaactcgg ccgcggtcac cagtagggga aaatgcagat gaacactatt caaaggacca
2701 tgatgtgaaa atggaggaac agagtattat ggcgcatatg ggcattgact caggaaccac
2761 taacattttt gatgaagtag acaagagtga ctttaaaact gactttggtt cggaatttcc
2821 aattttttct cctatgcctg gagaatcctg tgagaatgcc aacacttcgt tgggcagaag
2881 aatgtcagtt aattgtgaga agttggtgaa gagggaaaag ccaagggaat taatttttcc
2941 atctaattat gacctccttc gccacttaca gtatgcaaca cattttccta tacctctgga
3001 tgaatcttca agtcatgaat gtaaccagcg cacaatcctt ctctatggag tcggcaaaga
3061 gcgtgatgaa gcaaggcatc agctgaagaa gattaccaaa gatatcctga aaattctaaa
3121 taagaagagc accacagaga caggggttgg ggacgaagga caaaaagcca ggaagaacaa
3181 acaggagaca tttccaacac tggagactgt gttcactaaa ctccagctcc tttcatattt
3241 tgatcaacat caagtgacat ctcagatttc taacaatgtg ctagaacaaa tcacaagctt
3301 tgcgtcagga acatcctatc atctcccttt ggctcaccac attcagctca tctttgatct
3361 catggagcca gcactgaaca tcaacggact aattgacttc gcaatacagt tactaaatga
3421 actgagtgtt gtggaagctg aactgctcct aaaatcctcc agcctggcag gaagttatac
3481 aacaggactg tgtgtctgca tcgtggctgt tctcaggcgc tatcacagtt gtctaatctt
3541 gaatcctgat cagacagccc aggtgtttga aggtaagtat caaggttaca agacaggttt
3601 aaggagacca ataagctatt attcgtatag gatactttat acgaagttat taagttatgg
3661 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
3721 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
3781 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
3841 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
3901 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
3961 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
4021 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
4081 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
4141 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
4201 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
4261 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
4321 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
4381 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
4441 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
4501 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
4561 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
4621 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
4681 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
4741 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
4801 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
4861 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
4921 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
4981 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
5041 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
5101 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
5161 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
5221 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
5281 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
5341 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
5401 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
5461 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
5521 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
5581 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
5641 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
5701 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
5761 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
5821 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
5881 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
5941 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
6001 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
6061 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
6121 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
6181 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
6241 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
6301 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6361 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6421 ttgctcacat gt
//
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