| 质粒名称: | pYK465 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000817 |
| 产品用途: | Mammalian PhCMV-driven constitutive GNCR-VPR expression vector (PhCMV-GNCR-VPR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GNCR-VPR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK465
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK465载体质粒基本信息
pYK465质粒图谱载体图谱和pYK465载体序列质粒序列多克隆位点信息
pYK465质粒载体简介
pYK465质粒序列载体序列
LOCUS pYK465 7722 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7722)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7722
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..946
/label=oYF0019
primer_bind 881..918
/label=oYK421_GNCR_F
primer_bind 881..902
/label=oYK31-mCherry-NS3a-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 910..952
/label=oYF005
CDS 919..1617
/codon_start=1
/label=GNCR1-N
/translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
KAEEVKERCKS"
primer_bind 919..952
/label=oYF0011
primer_bind 919..943
/label=oLZ146 GNCR1-N F
primer_bind 919..943
/label=oSCL762
primer_bind 1585..1597
/label=oYK03-02-F
primer_bind 1585..1596
/label=oYK31-mCherry-NS3a-F
primer_bind complement(1596..1624)
/label=oYF006
primer_bind complement(1596..1624)
/label=oYF0020
primer_bind complement(1596..1618)
/label=oYF0012
primer_bind complement(1605..1643)
/label=oYK465_GNCR1_R
CDS 1624..3216
/label=Translation 748-6546
primer_bind 1624..1639
/label=oYK465_VPR_F
primer_bind 1624..1639
/label=oYK471_VPR_F
primer_bind 1624..1639
/label=oYK1255_VPR_F
misc_feature 1645..3213
/label=VPR
/label=nonstandard type: AD
CDS 1645..1794
/label=VP64
misc_feature 1645..1683
/label=nonstandard type: AD
misc_feature 1684..1722
/label=nonstandard type: AD
misc_feature 1723..1761
/label=nonstandard type: AD
primer_bind complement(1813..1839)
/label=oYK207_dcas9VP64_R
misc_feature 1819..1839
/label=NLS (SV40 short)
/label=nonstandard type: signal
misc_feature 2269..2625
/label=RelA (p65) AD
/label=nonstandard type: AD
CDS 2644..3213
/label=Rta AD
CDS 2644..3213
/label=Translation 5974-6543
primer_bind complement(3189..3242)
/label=oYK465_VPR_R
primer_bind complement(3220..3239)
/label=oYK207_dcas9VP64_R
terminator 3316..3543
/label=bGH PA
misc_feature 3589..4017
/label=f1 origin
misc_feature 4022..4365
/label=SV40 early promoter and origin
misc_feature 4211..4288
/label=SV40 ORI
CDS 4430..5221
/label=Kan/neoR
misc_feature 5395..5525
/label=SV40 early polyadenylation signal
primer_bind complement(5561..5581)
/label=M13-rev
misc_binding complement(5587..5609)
/label=LacO
promoter complement(5614..5643)
/label=lac
misc_feature complement(5908..6581)
/label=pUC origin
CDS complement(6729..7388)
/label=AmpR
promoter complement(7628..7656)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gctggctagg ccaccatgga catcgagaag ctgtgcaaga aggcggaaga ggaggccaag
961 gaggcccagg agaaggccga cgagctgaga caacgacacc ccgattcgca ggcggcggaa
1021 gacgcggagg atctcgcaaa tctggctgtc gccgccgtgc tgaccgcttg tctgctggcc
1081 caggagcatc ccaacgccga tatcgctaag ctgtgcatca aggccgcgag cgaagctgcg
1141 gaggccgcgt caaaagccgc cgagctggcc caacggcacc cagacagtca agcagccaga
1201 gatgctatca aactcgcctc tcaagccgcc cgcgccgtga tcctggccat catgctcgcc
1261 gcagagaatc ccaacgctga tattgcaaag ctatgtatta aggcggccag cgaagccgca
1321 gaggctgcct cgaaagctgc ggaacttgca cagcggcacc ccgactccca ggccgcccga
1381 gatgcaatta aactggccag tcaggccgct gaggcggtgg agagagccat ctggctggcc
1441 gccgagaacc ccaatgccga tatcgccaaa aaatgcatca aagcggcctc cgaagcagcc
1501 gaggaggcct ctaaagctgc cgaagaggct caaagacacc cagacagcca gaaggcacgc
1561 gacgagatca aggaggcctc tcagaaggcc gaggaggtga aggagagatg caagagcgcc
1621 agcgccagcg gttccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga
1681 agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt
1741 gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac
1801 tctagaagtt ccggatctcc gaaaaagaaa cgcaaagttg gtagccagta cctgcccgac
1861 accgacgacc ggcaccggat cgaggaaaag cggaagcgga cctacgagac attcaagagc
1921 atcatgaaga agtccccctt cagcggcccc accgacccta gacctccacc tagaagaatc
1981 gccgtgccca gcagatccag cgccagcgtg ccaaaacctg ccccccagcc ttaccccttc
2041 accagcagcc tgagcaccat caactacgac gagttcccta ccatggtgtt ccccagcggc
2101 cagatctctc aggcctctgc tctggctcca gcccctcctc aggtgctgcc tcaggctcct
2161 gctcctgcac cagctccagc catggtgtct gcactggctc aggcaccagc acccgtgcct
2221 gtgctggctc ctggacctcc acaggctgtg gctccaccag cccctaaacc tacacaggcc
2281 ggcgagggca cactgtctga agctctgctg cagctgcagt tcgacgacga ggatctggga
2341 gccctgctgg gaaacagcac cgatcctgcc gtgttcaccg acctggccag cgtggacaac
2401 agcgagttcc agcagctgct gaaccagggc atccctgtgg cccctcacac caccgagccc
2461 atgctgatgg aataccccga ggccatcacc cggctcgtga caggcgctca gaggcctcct
2521 gatccagctc ctgcccctct gggagcacca ggcctgccta atggactgct gtctggcgac
2581 gaggacttca gctctatcgc cgatatggat ttctcagcct tgctgggctc tggcagcggc
2641 agccgggatt ccagggaagg gatgtttttg ccgaagcctg aggccggctc cgctattagt
2701 gacgtgtttg agggccgcga ggtgtgccag ccaaaacgaa tccggccatt tcatcctcca
2761 ggaagtccat gggccaaccg cccactcccc gccagcctcg caccaacacc aaccggtcca
2821 gtacatgagc cagtcgggtc actgaccccg gcaccagtcc ctcagccact ggatccagcg
2881 cccgcagtga ctcccgaggc cagtcacctg ttggaggatc ccgatgaaga gacgagccag
2941 gctgtcaaag cccttcggga gatggccgat actgtgattc cccagaagga agaggctgca
3001 atctgtggcc aaatggacct ttcccatccg cccccaaggg gccatctgga tgagctgaca
3061 accacacttg agtccatgac cgaggatctg aacctggact cacccctgac cccggaattg
3121 aacgagattc tggatacctt cctgaacgac gagtgcctct tgcatgccat gcatatcagc
3181 acaggactgt ccatcttcga cacatctctg ttttgataag gatccactag tccagtgtgg
3241 tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
3301 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
3361 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
3421 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
3481 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
3541 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
3601 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
3661 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
3721 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
3781 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
3841 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
3901 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
3961 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
4021 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
4081 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
4141 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
4201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
4261 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
4321 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
4381 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
4441 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
4501 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
4561 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
4621 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
4681 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
4741 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
4801 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
4861 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
4921 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
4981 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
5041 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
5101 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
5161 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
5221 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
5281 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
5341 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
5401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
5461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
5521 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
5581 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
5641 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
5701 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
5761 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
5821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
5881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
5941 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
6001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
6061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
6121 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
6181 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
6241 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
6301 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
6361 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
6421 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
6481 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
6541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
6601 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
6661 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
6721 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
6781 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
6841 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
6901 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
6961 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
7021 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
7081 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
7141 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
7201 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
7261 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
7321 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
7381 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
7441 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
7501 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
7561 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
7621 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
7681 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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