| 质粒名称: | pAAVLINK-MAP1A-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001728 |
| 产品用途: | Body' AAVLINK plasmid for MAP1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MAP1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-MAP1A-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-MAP1A-B载体质粒基本信息
pAAVLINK-MAP1A-B质粒图谱载体图谱和pAAVLINK-MAP1A-B载体序列质粒序列多克隆位点信息
pAAVLINK-MAP1A-B质粒载体简介
pAAVLINK-MAP1A-B质粒序列载体序列
LOCUS pAAVLINK-MAP1A-B 7358 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7358)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7358
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4536
/codon_start=1
/label=MAP1A
/translation="VTPRSREAFGGRELGLQGKAPEKETSLFLSSLTTPAGATEHVSYI
QDETIPGYSETEQTISDEEIHDEPEERPAPPRFHTSTYDLPGPEGAGPFEASQPADSAV
PATSGKVYGTPETELTYPTNIVAAPLAEEEHVSSATSITECDKLSSFATSVAEDQSVAS
LTAPQTEETGKSSLLLDTVTSIPSSRTEATQGLDYVPSAGTISPTSSLEEDKGFKSPPC
EDFSVTGESEKRGEIIGKGLSGERAVEEEEEETANVEMSEKLCSQYGTPVFSAPGHALH
PGEPALGEAEERCLSPDDSTVKMASPPPSGPPSATHTPFHQSPVEEKSEPQDFQEADSW
GDTKRTPGVGKEDAAEETVKPGPEEGTLEKEEKVPPPRSPQAQEAPVNIDEGLTGCTIQ
LLPAQDKAIVFEIMEAGEPTGPILGAEALPGGLRTLPQEPGKPQKDEVLRYPDRSLSPE
DAESLSVLSVPSPDTANQEPTPKSPCGLTEQYLHKDRWPEVSPEDTQSLSLSEESPSKE
TSLDVSSKQLSPESLGTLQFGELNLGKEEMGHLMQAEDTSHHTAPMSVPEPHAATASPP
TDGTTRYSAQTDITDDSLDRKSPASSFSHSTPSGNGKYLPGAITSPDEHILTPDSSFSK
SPESLPGPALEDIAIKWEDKVPGLKDRTSEQKKEPEPKDEVLQQKDKTLEHKEVVEPKD
TAIYQKDEALHVKNEAVKQQDKALEQKGRDLEQKDTALEQKDKALEPKDKDLEEKDKAL
EQKDKIPEEKDKALEQKDTALEQKDKTLEPKDKDLEQKDRVLEQKEKIPEEKDKALDQK
VRSVEHKAPEDTVAEMKDRDLEQTDKAPEQKHQAQEQKDKVSEKKDQALEQKYWALGQK
DEALEQNIQALEENHQTQEQESLVQEDKTRKPKMLEEKSPEKVKAMEEKLEALLEKTKA
LGLEESLVQEGRAREQEEKYWRGQDVVQEWQETSPTREEPAGEQKELAPAWEDTSPEQD
NRYWRGREDVALEQDTYWRELSCERKVWFPHELDGQGARPHYTEERESTFLDEGPDDEQ
EVPLREHATRSPWASDFKDFQESSPQKGLEVERWLAESPVGLPPEEEDKLTRSPFEIIS
PPASPPEMVGQRVPSAPGQESPIPDPKLMPHMKNEPTTPSWLADIPPWVPKDRPLPPAP
LSPAPGPPTPAPESHTPAPFSWGTAEYDSVVAAVQEGAAELEGGPYSPLGKDYRKAEGE
REEEGRAEAPDKSSHSSKVPEASKSHATTEPEQTEPEQREPTPYPDERSFQYADIYEQM
MLTGLGPACPTREPPLGAAGDWPPCLSTKEAAAGRNTSAEKELSSPISPKSLQSDTPTF
SYAALAGPTVPPRPEPGPSMEPSLTPPAVPPRAPILSKGPSPPLNGNILSCSPDRRSPS
PKESGRSHWDDSTSDSELEK"
misc_feature 893..898
/label=SDS
misc_feature 1025..1030
/label=SDS
misc_feature 1274..1279
/label=SDS
misc_feature 1393..1398
/label=SDS
misc_feature 2330..2335
/label=SDS
misc_feature 2957..2962
/label=SDS
misc_feature 3086..3091
/label=SDS
misc_feature 3872..3877
/label=SDS
misc_feature 4208..4213
/label=SDS
misc_feature 4462..4467
/label=SDS
intron 4537..4577
/label=Splicing Donor
misc_feature 4581..4614
/label=lox2272-JT1571
repeat_region 4643..4761
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4643..4750
/label=AAV2 ITR
rep_origin 4836..5291
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5573..5677
/gene="bla"
/label=AmpR promoter
CDS 5678..6538
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6709..7297
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagca tacattatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtaactccaa ggagccggga ggcttttggg
301 ggtcgggaat tgggactcca gggcaaggcc cctgagaagg agacctcgtt attcctaagc
361 agcctgacca cacctgcagg agccactgag catgtctctt acatccagga tgagacaatc
421 cctggctact cagagactga gcagaccatc tcagatgagg agatccatga tgagccggag
481 gagcgcccag ctccacccag atttcataca agtacatatg acctgcctgg gcctgaaggt
541 gctggcccat tcgaagccag ccaacctgcc gatagtgctg ttcctgctac ctctggcaaa
601 gtctatggaa cgccagagac tgaactcacc taccccacta acatagtggc tgcccctttg
661 gctgaagagg aacatgtgtc ctcggccact tcaatcactg agtgtgacaa actttcttcc
721 tttgccacat cagtggctga ggaccaatct gtggcctcac ttacagctcc ccagacagag
781 gagacaggca agagctccct gctgcttgac acagtcacaa gcatcccttc ctcccgtact
841 gaagctacgc agggcttgga ctatgtgcca tcagctggta ccatctcacc cacctcctca
901 ctggaagaag acaagggctt caaatcacca ccctgtgagg acttctctgt gactggggag
961 tcagagaaga gaggagagat catagggaaa ggcttgtctg gagagagagc tgtggaagag
1021 gaagaggagg agacagcaaa cgtagagatg tctgagaaac tttgcagtca atatggaact
1081 ccagtgttta gtgcccctgg gcatgcccta catccaggag aaccagccct tggagaagca
1141 gaggagcggt gccttagccc agatgacagc acagtgaaga tggcttctcc tccaccatct
1201 ggcccaccca gtgccaccca cacacccttt catcagtccc cagtggaaga aaagtctgag
1261 ccccaagact ttcaggaggc agactcctgg ggagacacta agcgcacacc aggtgtgggc
1321 aaagaagatg ctgctgagga gacagtcaag ccagggcctg aagagggcac actagagaag
1381 gaagagaaag ttcctcctcc caggagcccc caggcccagg aagcacctgt caacattgat
1441 gagggactta caggctgtac cattcaactg ttgccagcac aggataaagc aatagtcttt
1501 gagattatgg aggcaggaga gcccacaggc ccaattctgg gagcagaagc ccttcccgga
1561 ggtttgagga ctttacccca agaacctggc aaacctcaga aagatgaggt gctcagatat
1621 cctgaccgaa gcctctctcc tgaagatgca gaatccctct ctgtcctcag cgtgccctcc
1681 ccagacactg ccaaccaaga gcctaccccc aagtctccct gtggcctgac agaacagtac
1741 ctacacaaag accgttggcc agaggtatct ccagaagaca cccagtcact ttctctgtca
1801 gaagagagtc ccagcaagga gacctccctg gatgtctctt ctaagcagct ctctccagaa
1861 agccttggca ccctccagtt tggggaacta aaccttggga aggaagaaat ggggcatctg
1921 atgcaggccg aggacacctc tcaccacaca gctcccatgt ctgttccaga gccccatgca
1981 gccacagcgt cacctcccac agatgggaca actcgatact ctgcacagac agacatcaca
2041 gatgacagcc ttgacaggaa gtcacctgcc agctcattct ctcactctac accttcagga
2101 aatgggaagt acttacctgg ggcgatcaca agccctgatg aacacattct gacacctgat
2161 agctccttct ccaagagtcc tgagtctttg ccaggccctg ccttggagga cattgccata
2221 aagtgggaag ataaagttcc agggttgaaa gacagaacct cagaacagaa gaaggaacct
2281 gagccaaagg atgaagtttt acagcagaaa gacaaaactc tggagcacaa ggaggtggta
2341 gagccgaagg atacagccat ctatcagaaa gatgaggctc tgcatgtaaa gaatgaggct
2401 gtgaaacagc aggataaggc tttagaacaa aagggcagag acttagagca aaaagacaca
2461 gccctagaac agaaggacaa ggccctggaa ccaaaagaca aagacttaga agaaaaagac
2521 aaggccctgg aacagaagga taagattcca gaagagaaag acaaagcctt agaacaaaag
2581 gatacagccc tggaacagaa ggacaagacc ctggaaccaa aagataaaga cttggaacaa
2641 aaggacaggg tcctagaaca gaaggagaag atcccagaag agaaagacaa agccttagat
2701 caaaaagtca gaagtgttga acataaggct ccggaggaca cggtcgctga aatgaaggac
2761 agagacctag aacagacaga caaagcccct gaacagaaac accaggccca ggaacaaaag
2821 gataaagtct cagaaaagaa ggatcaggcc ttagaacaaa aatactgggc tttgggacag
2881 aaggatgaag ccctggaaca aaacattcag gctctggaag agaaccacca aactcaggag
2941 caggagagcc tagtgcagga ggataaaacc aggaaaccaa agatgctaga ggaaaaatcc
3001 ccagaaaagg tcaaggccat ggaagagaag ttagaagctc ttctggagaa gaccaaagct
3061 ctgggcctgg aagagagcct agtgcaggag ggcagggcca gagagcagga agaaaagtac
3121 tggagggggc aggatgtggt ccaggagtgg caagaaacat ctcctaccag agaggagccg
3181 gctggagaac agaaagagct tgccccggca tgggaggaca catctcctga gcaggacaat
3241 aggtattgga ggggcagaga ggatgtggcc ttggaacagg acacatactg gagggagcta
3301 agctgtgagc ggaaggtctg gttccctcac gagctggatg gccagggggc ccgcccacac
3361 tacactgagg aacgggaaag cactttccta gatgagggcc cagatgatga gcaagaagta
3421 cccctgcggg aacacgcaac ccggagcccc tgggcctcag acttcaagga tttccaggaa
3481 tcctcaccac agaaggggct agaggtggag cgctggcttg ctgaatcacc agttgggttg
3541 ccaccagagg aagaggacaa actgacccgc tctccctttg agatcatctc ccctccagct
3601 tccccacctg agatggttgg acaaagggtt ccttcagccc caggacaaga gagtcctatc
3661 ccagacccta agctcatgcc acacatgaag aatgaaccca ctactccctc atggctggct
3721 gacatcccac cctgggtgcc caaggacaga cccctccccc ctgcacccct ctccccagct
3781 cctggtcccc ccacacctgc cccggaatcc catactcctg cacccttctc ttggggcaca
3841 gccgagtatg acagtgtggt ggctgcagtg caggaggggg cagctgagtt ggaaggtggg
3901 ccatactccc ccctggggaa ggactaccgc aaggctgaag gggaaaggga agaagaaggt
3961 agggctgagg ctcctgacaa aagctcacac agctcaaagg taccagaggc cagcaaaagc
4021 catgccacca cggagcctga gcagactgag ccggagcaga gagagcccac accctatcct
4081 gatgagagaa gctttcagta tgcagacatc tatgagcaga tgatgcttac tgggcttggc
4141 cctgcatgcc ccactagaga gcctccactt ggagcagctg gggattggcc cccatgcctc
4201 tcaaccaagg aggcagctgc cggccgaaac acatctgcag agaaggagct ttcatctcct
4261 atctcaccca agagcctcca gtctgacact ccaaccttca gctatgcagc cctggcagga
4321 cccactgtac ccccaaggcc agagccaggg ccaagtatgg agcccagcct caccccacct
4381 gcagttcccc cccgtgctcc tatcctgagc aaaggcccaa gcccccctct taatggtaac
4441 atcctgagct gcagcccaga taggaggtcc ccatccccca aggaatcagg ccggagtcac
4501 tgggatgaca gcactagtga ctcagaactg gagaaggtaa gtatcaaggt tacaagacag
4561 gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag ttatgaattc
4621 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
4681 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccggcctca gtgagcgagc
4741 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
4801 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
4861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
4921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
4981 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
5041 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
5101 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
5161 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
5221 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
5281 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
5341 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
5401 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
5461 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
5521 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
5581 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
5641 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
5701 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
5761 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
5821 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
5881 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
5941 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
6001 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
6061 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
6121 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
6181 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
6241 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
6301 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
6361 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
6421 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
6481 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
6541 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
6601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
6661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
6721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
6781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
6841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
6901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
6961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
7021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
7081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
7141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
7201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
7261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
7321 acggttcctg gccttttgct ggccttttgc tcacatgt
//
