pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDC42BPB-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CDC42BPB-FLAG               6294 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3007
                     /codon_start=1
                     /gene="CDC42BPB"
                     /gene_synonym="CHOCNS; MRCKB"
                     /product="serine/threonine-protein kinase MRCK betaisoform 
                     1"
                     /EC_number="2.7.11.1"
                     /label=CDC42BPB
                     /note="isoform 1 is encoded by transcript variant 1; 
                     serine/threonine-protein kinase MRCK beta; myotonic 
                     dystrophy protein kinase-like beta; DMPK-like beta; 
                     myotonic dystrophy kinase-related CDC42-binding kinase 
                     beta; CDC42BP-beta; CDC42 binding protein kinase beta 
                     (DMPK-like)"
                     /db_xref="GI:115527097"
                     /db_xref="CCDS:CCDS9978.1"
                     /db_xref="GeneID:9578"
                     /db_xref="HGNC:HGNC:1738"
                     /db_xref="MIM:614062"
                     /protein_id="NP_006026.3"
                     /translation="VVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTR
                     DVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGC
                     QLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLC
                     VGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGL
                     YVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRP
                     LNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEE
                     RLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPA
                     PTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNS
                     SNPSGPPSPNSPHRSQLPLEGLEQPACDT"
     misc_feature    1756..1761
                     /label=SDS
     misc_feature    2413..2418
                     /label=SDS
     misc_feature    2721..2726
                     /label=SDS
     CDS             3014..3079
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3083..3514
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3227..3232
                     /label=SDS
     repeat_region   3557..3697
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3557..3686
                     /label=AAV2 ITR
     repeat_region   3558..3698
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3772..4227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4509..4613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4614..5474
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5645..6233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtcg tgcatgttcc
     1561 cttggaagcc tacgacagct cgctgcctct catcaaggcc atcctgacag ctgccatcgt
     1621 ggatgcagac aggattgcag tcggcctaga agaagggctc tatgtcatag aggtcacccg
     1681 agatgtgatc gtccgtgccg ctgactgtaa gaaggtacac cagatcgagc ttgctcccag
     1741 ggagaagatc gtaatcctcc tctgtggccg gaaccaccat gtgcacctct atccgtggtc
     1801 gtcccttgat ggagcggaag gcagctttga catcaagctt ccggaaacca aaggctgcca
     1861 gctcatggcc acggccacac tcaagaggaa ctctggcacc tgcctgtttg tggccgtgaa
     1921 acggctgatc ctttgctatg agatccagag aacgaagcca ttccacagaa agttcaatga
     1981 gattgtggct cccggcagcg tgcagtgcct ggcggtgctc agggacaggc tctgtgtggg
     2041 ctacccttct gggttctgcc tgctgagcat ccagggggac gggcagcctc taaacctggt
     2101 aaatcccaat gacccctcgc ttgcgttcct ctcacaacag tcttttgatg ccctttgtgc
     2161 tgtggagctc gaaagcgagg agtacctgct ttgcttcagc cacatgggac tgtacgtgga
     2221 cccgcaaggc cggagggcac gcgcgcagga gctcatgtgg cctgcggctc ctgtcgcctg
     2281 tagttgcagc cccacccacg tcacggtgta cagcgagtat ggcgtggacg tctttgatgt
     2341 gcgcaccatg gagtgggtgc agaccatcgg cctgcggagg ataaggcccc tgaactctga
     2401 aggcaccctc aacctcctca actgcgagcc tccacgcttg atctacttca agagcaagtt
     2461 ctcgggagcg gttctcaacg tgccggacac ctccgacaac agcaagaagc agatgctgcg
     2521 caccaggagc aaaaggcggt tcgtcttcaa ggtcccagag gaagagagac tgcagcagag
     2581 gcgagagatg cttagagacc cagaattgag atccaaaatg atatccaacc caaccaactt
     2641 caaccacgtg gcccacatgg gcccaggcga cggcatgcag gtgctcatgg acctgcctct
     2701 gagtgctgtg cccccctccc aggaggaaag gccgggcccc gctcccacca acctggctcg
     2761 ccagcctcca tccaggaaca agccctacat ctcgtggccc tcatcaggtg gatcggagcc
     2821 tagcgtgact gtgcctctga gaagtatgtc tgatccagac caggactttg acaaagagcc
     2881 tgattcggac tccaccaaac actcaactcc atcgaatagc tccaacccca gcggcccacc
     2941 gagccccaac tccccccaca ggagccagct ccccctcgaa ggcctggagc agccggcctg
     3001 tgacacctgt acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta
     3061 caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa
     3121 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3181 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3241 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3301 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
     3361 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3421 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3481 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
     3541 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3601 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3661 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3721 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3781 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3841 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3901 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3961 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     4021 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4081 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4141 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4201 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4261 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4321 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4381 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4441 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4501 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4561 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4621 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4681 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4741 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4801 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4861 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4921 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4981 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5041 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5101 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5161 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5221 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5281 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5341 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5401 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5461 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5521 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5581 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//