| 质粒名称: | Apple-53BP1trunc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69531 |
| 存储实验室: | Ralph Weissleder |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX |
| 载体出品公司: | Clontech |
| 空载体大小: | 8003 bp |
| 完整质粒大小: | 10184 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | 53BP1,TP53BP1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1473 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | Apple fluorescent protein(插入物上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BsrGI (未破坏) |
| 3’克隆位点: | XbaI（克隆期间销毁） |
| 5’测序引物 | CMV-F |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Apple-53BP1trunc was a gift from Ralph Weissleder (Addgene plasmid # 69531 ; http://n2t.net/addgene:69531 ; RRID:Addgene_69531) |
| 参考文献部分: | Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Yang KS, Kohler RH, Landon M, Giedt R, Weissleder R. Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129. 10.1038/srep10129 PubMed 25984718 |
买家导航
Apple-53BP1trunc载体 Apple53BP1trunc质粒基本信息
Apple-53BP1trunc质粒图谱 Apple53BP1trunc载体图谱和Apple-53BP1trunc载体序列 Apple53BP1trunc质粒序列多克隆位点信息
Apple-53BP1trunc质粒 Apple53BP1trunc载体简介
Apple-53BP1trunc质粒序列 Apple53BP1trunc载体序列
LOCUS Apple-53BP1trunc 10961 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Fluorescent reporter for DNA damage.
ACCESSION .
VERSION .
KEYWORDS Apple-53BP1trunc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10961)
AUTHORS Yang KS, Kohler RH, Landon M, Giedt R, Weissleder R
TITLE Single cell resolution in vivo imaging of DNA damage following PARP
inhibition.
JOURNAL Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129.
PUBMED 25984718
REFERENCE 2 (bases 1 to 10961)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10961
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2659..2679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2705..2729
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2820..3527
/codon_start=1
/product="photostable monomeric derivative of DsRed (Shaner
et al., 2008)"
/label=mApple
/note="mammalian codon-optimized"
/translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind 3233..3252
/label=mCherry-F
/note="mCherry, forward primer"
promoter 5019..5518
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5054..5077)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5451..5470
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5539..6138
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5539..5558)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6035..6055
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6152..6740
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6205..6225)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6623..6634)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6947..7580
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7709..7725)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7709..7725)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7722..7744)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7733..7749
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7757..7787)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7802..7823
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7940..7957)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8111..8699)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8191..8210)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8870..9730)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9493..9512
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
mobile_element 9741..10508
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
promoter complement(10509..10612)
/gene="bla"
/label=AmpR promoter
polyA_signal 10660..10794
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10697..10716)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10751..10770
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccgggata gctagcacca
2821 tggtgagcaa gggcgaggag aataacatgg ccatcatcaa ggagttcatg cgcttcaagg
2881 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
2941 gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
3001 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggtctac attaagcacc
3061 cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg tgggagcgcg
3121 tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc ctgcaggacg
3181 gcgtgttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
3241 tgcagaagaa gaccatgggc tgggaggcct ccgaggagcg gatgtacccc gaggacggcg
3301 ccctgaagag cgagatcaag aagaggctga agctgaagga cggcggccac tacgccgccg
3361 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacatcgtcg
3421 acatcaagtt ggacatcgtg tcccacaacg aggactacac catcgtggaa cagtacgaac
3481 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggga gaagaagagt
3541 ttgatatgcc tcagcctcca catggccatg tcttacatcg tcacatgaga acaatccggg
3601 aagtacgcac acttgtcact cgtgtcatta cagatgtgta ttatgtggat ggaacagaag
3661 tagaaagaaa agtaactgag gagactgaag agccaattgt agagtgtcag gagtgtgaaa
3721 ctgaagtttc cccttcacag actgggggct cctcaggtga cctgggggat atcagctcct
3781 tctcctccaa ggcatccagc ttacaccgca catcaagtgg gacaagtctc tcagctatgc
3841 acagcagtgg aagctcaggg aaaggagccg gaccactcag agggaaaacc agcgggacag
3901 aacccgcaga ttttgcctta cccagctccc gaggaggccc aggaaaactg agtcctagaa
3961 aaggggtcag tcagacaggg acgccagtgt gtgaggagga tggtgatgca ggccttggca
4021 tcagacaggg agggaaggct ccagtcacgc ctcgtgggcg tgggcgaagg ggccgcccac
4081 cttctcggac cactggaacc agagaaacag ctgtgcctgg ccccttgggc atagaggaca
4141 tttcacctaa cttgtcacca gatgataaat ccttcagccg tgtcgtgccc cgagtgccag
4201 actccaccag acgaacagat gtgggtgctg gtgctttgcg tcgtagtgac tctccagaaa
4261 ttcctttcca ggctgctgct ggcccttctg atggcttaga tgcctcctct ccaggaaata
4321 gctttgtagg gctccgtgtt gtagccaagt ggtcatccaa tggctacttt tactctggga
4381 aaatcacacg agatgtcgga gctgggaagt ataaattgct ctttgatgat gggtacgaat
4441 gtgatgtgtt gggcaaagac attctgttat gtgaccccat cccgctggac actgaagtga
4501 cggccctctc ggaggatgag tatttcagtg caggagtggt gaaaggacat aggaaggagt
4561 ctggggaact gtactacagc attgaaaaag aaggccaaag aaagtggtat aagcgaatgg
4621 ctgtcatcct gtccttggag caaggaaaca gactgagaga gcagtatggg cttggcccct
4681 atgaagcagt aacacctctt acaaaggcag cagatatcag cttagacaat ttggtggaag
4741 ggaagcggaa acggcgcagt aacgtcagct ccccagccac ccctactgcc tccagtagca
4801 gcagcacaac ccctacccga aagatcacag aaagtcctcg tgcctccatg ggagttctct
4861 caggcaaaag aaaacttatc acttctgaag aggaacggtc ccctgccaag cgaggtcgca
4921 agtctgccac agtaaaacct ggtgcagtag gggcaggaga gtttgtgagc ccctgtgaga
4981 gtggagacaa caccgtgaat gatctagata attctaccgg gtaggggagg cgcttttccc
5041 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
5101 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
5161 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
5221 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
5281 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
5341 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
5401 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
5461 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
5521 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
5581 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
5641 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
5701 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
5761 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
5821 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
5881 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
5941 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
6001 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
6061 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
6121 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
6181 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
6241 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
6301 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
6361 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
6421 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
6481 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
6541 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
6601 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
6661 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
6721 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
6781 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
6841 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
6901 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
6961 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
7021 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
7081 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
7141 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
7201 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
7261 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
7321 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
7381 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
7441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
7501 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
7561 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
7621 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
7681 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
7741 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
7801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
7861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
7921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
7981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
8041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
8101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
8161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
8221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
8281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
8341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
8401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
8461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
8521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
8581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
8641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
8701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
8761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
8821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
8881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
8941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
9001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
9061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
9121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
9181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
9241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
9301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
9361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
9421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
9481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
9541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
9601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
9661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
9721 gaatactcat actcttcctt ggtgatgctg ccaacttact gatttagtgt atgatggtgt
9781 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
9841 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
9901 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
9961 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
10021 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
10081 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
10141 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
10201 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
10261 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
10321 agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg cataacctga
10381 atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca aaatcggtgg
10441 agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa taagttggag
10501 tcattaccct cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
10561 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
10621 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
10681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
10741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
10801 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
10861 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
10921 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
