| 质粒名称: | pYK231 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000806 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-MCP-mCherry-2xANR expression vector (PhCMV-NLS-MCP-mCherry-2xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-MCP-mCherry-2xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK231
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK231载体质粒基本信息
pYK231质粒图谱载体图谱和pYK231载体序列质粒序列多克隆位点信息
pYK231质粒载体简介
pYK231质粒序列载体序列
LOCUS pYK231 6877 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6877)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6877
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..994
/label=oYK57-NLS-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK31-mCherry-NS3a-F
misc_feature 905..964
/label=NLS2
primer_bind complement(943..967)
/label=oYK163_NLS_R
primer_bind complement(945..987)
/label=oYK57-NLS-R
primer_bind complement(945..984)
/label=oYK54-NLS-R
primer_bind complement(945..984)
/label=oYK167_NLS_R
primer_bind complement(945..969)
/label=oYK164_NLS_R
primer_bind 964..985
/label=oYK05-mcp-F
primer_bind 965..991
/label=pYK54-Mcp-F
primer_bind 965..990
/label=oYK77_mcpns3a_F
CDS 968..1354
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 968
/codon_start=1
/label=ANR
/translation=""
regulatory 968
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1334..1384)
/label=oSCL093
primer_bind complement(1334..1354)
/label=Oscl32-2
primer_bind complement(1354..1380)
/label=oDJ171-4
primer_bind 1354..1374
/label=oLZ048 NSP3-F
primer_bind 1355..1414
/label=oDJ171-3
misc_feature 1355..1390
/label=linker
misc_feature 1355..1389
/label=linker
primer_bind 1355..1384
/label=oSCL388
primer_bind 1355..1374
/label=oSCL1192
primer_bind 1355..1372
/label=oLZ147 NS3a F
primer_bind 1355..1371
/label=oYF0033
primer_bind complement(1365..1402)
/label=oSCL1119
primer_bind complement(1365..1384)
/label=oSCL075
primer_bind complement(1373..1400)
/label=oYK05-mcp-R
primer_bind complement(1373..1391)
/label=oYK82_mcp_R
primer_bind 1375..1409
/label=oYK03-02-F
primer_bind 1381..1408
/label=oYK05-mcherry-F
primer_bind 1385..1417
/label=omz_linker_mCherry_F
CDS 1391..2095
/codon_start=1
/label=mCherry
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
primer_bind 1391..1411
/label=oXQ135
primer_bind 1391..1411
/label=oXQ174
primer_bind 1391..1411
/label=omz_mCherry_F
primer_bind 1391..1408
/label=oYK31-mCherry-NS3a-F
misc_feature 1391..1393
/label=Coh2
primer_bind complement(1703..1714)
/label=oSCL011
primer_bind complement(1703..1714)
/label=omz_Coh2_R
primer_bind complement(1703..1714)
/label=omz_Coh2_His_R
primer_bind complement(2074..2115)
/label=oYK83_mcp_mcherry_R
primer_bind complement(2075..2128)
/label=oDJ171-4
primer_bind complement(2075..2097)
/label=oXQ136
primer_bind complement(2075..2095)
/label=oXQ161
primer_bind complement(2075..2095)
/label=omz_mCherry_His_R
primer_bind complement(2075..2095)
/label=oYK05-mcherry-R
primer_bind complement(2077..2115)
/label=omz_mCherry_linker_R
CDS 2096..2170
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2096..2115)
/label=oYK82_mcp_R
primer_bind complement(2096..2115)
/label=oYK195_ANR_R
primer_bind 2096..2110
/label=oYK82_LinkANR_F
primer_bind complement(2156..2170)
/label=oYK89_mCherry_R
CDS 2171..2233
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2171..2186
/label=Primer 4
primer_bind complement(2210..2253)
/label=oYK195_ANR_R
primer_bind complement(2215..2233)
/label=oYK82_LinkANR_R
primer_bind 2218..2232
/label=Primer 3
CDS 2234..2308
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(2234..2253)
/label=oYK82_mcp_R
primer_bind complement(2234..2253)
/label=oYK83_mcp_mcherry_R
primer_bind 2234..2248
/label=oYK82_LinkANR_F
primer_bind complement(2294..2308)
/label=oYK89_mCherry_R
CDS 2309..2371
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2309..2324
/label=Primer 4
primer_bind complement(2348..2371)
/label=oYK195_ANR_R
primer_bind complement(2353..2394)
/label=oYK82_LinkANR_R
primer_bind 2356..2370
/label=Primer 3
primer_bind complement(2372..2394)
/label=oYK05-mcherry-R
primer_bind complement(2375..2397)
/label=oYK57-MCP-mCherry-R
primer_bind complement(2398..2415)
/label=oXQ161
terminator 2471..2698
/label=bGH PA
misc_feature 2744..3172
/label=f1 origin
misc_feature 3177..3520
/label=SV40 early promoter and origin
misc_feature 3366..3443
/label=SV40 ORI
CDS 3585..4376
/label=Kan/neoR
misc_feature 4550..4680
/label=SV40 early polyadenylation signal
primer_bind complement(4716..4736)
/label=M13-rev
misc_binding complement(4742..4764)
/label=LacO
promoter complement(4769..4798)
/label=lac
misc_feature complement(5063..5736)
/label=pUC origin
CDS complement(5884..6543)
/label=AmpR
promoter complement(6783..6811)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatggct tcaaacttta ctcagttcgt gctcgtggac aatggtggga caggggatgt
1021 gacagtggct ccttctaatt tcgctaatgg ggtggcagag tggatcagct ccaactcacg
1081 gagccaggcc tacaaggtga catgcagcgt caggcagtct agtgcccaga agagaaagta
1141 taccatcaag gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc
1201 tgtcgccgct tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa
1261 ttctgactgt gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat
1321 cccttccgcc atcgccgcta actcaggtat ctacgccagc ggaagtggcg gaggaggcga
1381 cgtcggatcc gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
1441 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
1501 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
1561 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
1621 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
1681 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
1741 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
1801 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
1861 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
1921 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
1981 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
2041 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggctc
2101 cggaacgggc agcgggaccg gaagtggcac cggaaccact tccggaaccg gtactggcgg
2161 gagtaccggc ggtgagctag acgaactcgt gtacctgttg gatggccccg gttacgaccc
2221 aatccacagc gatggctccg gaacgggcag cgggaccgga agtggcaccg gaaccacttc
2281 cggaaccggt actggcggga gtaccggcgg tgagctagac gaactcgtgt acctgttgga
2341 tggccccggt tacgacccaa tccacagcga ttaaggatcc actagtccag tgtggtggaa
2401 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc
2461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
2521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
2581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
2641 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
2701 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
2761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
2821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
2881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
2941 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
3001 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
3061 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
3121 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
3181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
3301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
3361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
3421 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
3481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
3541 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
3601 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
3661 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
3721 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
3781 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
3841 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
3901 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
3961 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
4021 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
4081 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
4141 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
4201 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
4261 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
4321 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
4381 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
4441 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
4501 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
4561 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
4621 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
4681 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
4741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
4801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
4861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
4921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
4981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
5041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
5101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
5161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
5221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
5281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
5341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
5401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
5461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
5521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
5581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
5641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
5701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
5761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
5821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
5881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
5941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
6001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
6061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
6121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
6181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
6241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
6301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
6361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
6421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
6481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
6541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
6601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
6661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
6721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
6781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
6841 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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