| 质粒名称: | UBQ:RUBY |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 160909 |
| 存储实验室: | Yunde Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | pHDE |
| 载体抗性: | 大观霉素 |
| 空载体大小: | 9346 bp |
| 完整质粒大小: | 15550 bp |
| 载体类型: | 植物表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RUBY and UBIQUITIN promoter |
| 物种来源: | Synthetic |
| 插入大小: | 6205 bp |
| 启动子 | UBIQUITIN |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | CCTGTCAAACACTGATAGTTTctgcagtgcagcgtgacccggtc |
| 3' 测序引物 | GCTTACTCAGTTAGGTCTAGCTTATCTTTAATCATATTCCATAGTCCA |
|
文献引用方法 |
|
| 材料和方法部分: | UBQ:RUBY was a gift from Yunde Zhao (Addgene plasmid # 160909 ; http://n2t.net/addgene:160909 ; RRID:Addgene_160909) |
| 参考文献部分: | A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566 |
买家导航
UBQ:RUBY载体 UBQRUBY质粒基本信息
UBQ:RUBY质粒图谱 UBQRUBY载体图谱和UBQ:RUBY载体序列 UBQRUBY质粒序列多克隆位点信息
UBQ:RUBY质粒 UBQRUBY载体简介
UBQ:RUBY质粒序列 UBQRUBY载体序列
LOCUS UBQ:RUBY 15623 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION RUBY under the control of Ubiquitin promoter/marker for
transformation.
ACCESSION .
VERSION .
KEYWORDS UBQ:RUBY
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15623)
AUTHORS He Y, Zhang T, Sun H, Zhan H, Zhao Y
TITLE A reporter for noninvasively monitoring gene expression and plant
transformation.
JOURNAL Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1.
eCollection 2020.
PUBMED 33024566
REFERENCE 2 (bases 1 to 15623)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15623
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 134..158
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3646..3702
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4537..4593
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
promoter 6800..6979
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 6832..7015
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
terminator 8130..8382
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(8957..8974)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8957..8973)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8966..8988)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 9195..9219
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 9740..10531
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 10777..11365
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11266..11285
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11519..11536
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 11551..11691
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(11617..11636)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 11777..11799
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 12035..12229
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(12295..13368)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(13797..14426)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 taaagtactt taaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
61 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
121 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttctgcagtg
181 cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa
241 aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata
301 catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg
361 ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg
421 acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa
481 atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg
541 ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta
601 aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag
661 aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg
721 aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta
781 acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg
841 catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgcgt
901 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
961 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
1021 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
1081 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
1141 cgtcctcccc cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc
1201 cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg
1261 ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca
1321 gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc
1381 atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata
1441 tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga
1501 tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt
1561 ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa
1621 gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat
1681 gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc
1741 gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat
1801 tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa
1861 tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg
1921 catatgcagc atctattcat atgctctaac cttgagtacc tatctattat aataaacaag
1981 tatgttttat aattattttg atcttgatat acttggatga tggcatatgc agcagctata
2041 tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg
2101 tcgatgctca ccctgttgtt tggtgttact tctgcagaaa ctagtatgga tcatgcgacc
2161 ctcgccatga tcctcgcgat ctggttcatc agcttccact tcatcaagct gctgttctcc
2221 cagcagacca ccaagctgct tccgccagga ccaaagccgc ttccgatcat cggcaacatc
2281 cttgaggtgg gcaagaagcc gcatcggtcc ttcgccaacc tcgccaagat tcacggccca
2341 ctcatttccc tcagactcgg ctctgtgacc accatcgttg tgtcctctgc cgacgtggcc
2401 aaagagatgt tcctcaagaa ggatcacccg ctctccaacc gcacgatccc gaatagtgtt
2461 acagccggcg accaccacaa gctcaccatg tcttggctcc cggtgtctcc gaagtggcgc
2521 aacttccgca agattaccgc cgtgcatctg ctctccccac agagactcga tgcctgccag
2581 acattcaggc acgccaaggt gcagcagctc tacgagtacg ttcaagagtg cgcccagaaa
2641 ggccaggccg tggatattgg caaggccgcc tttacgacca gcctcaacct cctcagcaag
2701 ctgttcttca gcgtcgagct ggcgcaccac aagtcccata ccagccaaga gttcaaagag
2761 ctgatctgga acatcatgga agatataggc aagccgaact acgccgacta cttcccgatt
2821 ctcggctgcg ttgacccatc tggcattaga agaaggctcg cctgctcctt cgacaagctg
2881 atcgccgtgt tccagggcat catctgcgag agactcgccc cagattcctc caccacaact
2941 accaccacca ccgacgacgt gctcgatgtg ctcctccagc tgttcaagca gaacgagctg
3001 acgatgggcg agatcaacca cctcctcgtg gacatcttcg acgccggcac cgataccaca
3061 tcctccacat tcgagtgggt gatgaccgag ctgatccgca atccagagat gatggaaaag
3121 gcccaagagg aaatcaagca ggtcctcggc aaggacaagc agatccaaga gtccgacatc
3181 atcaacctgc cgtacctcca ggcgatcatc aaagagacac tccgcctcca tccgccgacc
3241 gtgttcttgc tcccaagaaa ggccgacacc gatgtcgagc tgtacggcta catcgtgccg
3301 aaggatgccc agatcctcgt gaacctctgg gccattggca gggacccaaa cgcctggcag
3361 aacgccgata ttttcagccc agagcgcttc atcggctgcg agatcgatgt taagggccgc
3421 gatttcggcc tccttccatt tggcgctggc cgcagaattt gcccaggcat gaatctcgcc
3481 atcaggatgc tcaccctcat gctcgccaca ctcctccagt tcttcaactg gaagctcgaa
3541 ggcgacatct ccccgaagga cctcgacatg gacgagaagt tcggcattgc gctccaaaag
3601 accaagccgc tcaagctcat cccgattccg cgctacggta gcggagctac caattttagc
3661 ctccttaagc aggcaggtga tgtagaagag aaccccgggc ctatgaagat gatgaacggc
3721 gaggacgcca acgaccagat gatcaaagag tccttcttca tcacccacgg caacccgatc
3781 ctcaccgtcg aggatacaca tccgctcagg ccgttcttcg agacatggcg cgagaagatt
3841 ttctccaaga agccgaaggc catcctcatc atctccggcc actgggagac agtgaagcca
3901 accgtgaacg ccgtgcacat caacgacacc atccacgact tcgacgacta cccagccgcc
3961 atgtaccagt tcaagtaccc agctccaggc gagccagagc ttgcgagaaa ggtggaagag
4021 atcctcaaga agtccgggtt cgagacagcc gagacagacc aaaagagggg ccttgatcac
4081 ggcgcctggg ttccactcat gctcatgtat ccagaggcgg acatcccggt gtgccagctc
4141 tcagttcagc cacatctcga cggcacctac cactacaatc tcggcagagc cctcgcgccg
4201 ctcaagaatg atggcgtgct cattattggc tccggcagcg ccacacatcc actcgatgag
4261 acaccgcact acttcgatgg tgttgcccct tgggccgctg ccttcgattc ttggcttagg
4321 aaggccctca tcaacggccg cttcgaggaa gtgaacatct acgagagcaa ggccccgaac
4381 tggaagctcg cccatccatt tccagagcac ttctacccgc tccacgttgt gctcggcgct
4441 gctggtgaaa agtggaaggc cgagctgatc cactcctcct gggatcatgg cacactttgc
4501 cacggctcct acaagttcac ctccgccgga tccggagcaa ccaactttag cctgctcaag
4561 caagcaggag atgttgagga aaatcctggc cccatgaccg ccatcaagat gaacaccaac
4621 ggcgagggcg agacacagca catcctcatg atcccgttca tggcgcaggg ccacctcagg
4681 ccatttctcg aactcgccat gttcctctac aagcgctccc acgtgatcat caccctgctc
4741 acaactccgc tcaatgccgg cttcctcagg cacctccttc accaccattc ctactcctcc
4801 agcggcatca ggatcgtcga gctgccattc aactccacca accacggact cccaccgggc
4861 atcgagaaca ccgataagct cacactcccg ctcgtggtgt ccctcttcca ttccaccatc
4921 agcctcgatc cgcacctccg cgattacatc tccaggcatt tcagcccagc caggccacca
4981 ctctgcgtga tccatgatgt gttcctcggc tgggttgacc aggtggccaa ggatgtgggc
5041 tctacaggcg tggtgttcac aacaggcggc gcttatggca catccgccta cgtgtccatc
5101 tggaacgatc tcccgcacca gaactactcc gacgaccaag agttcccgct gccaggcttc
5161 ccagagaacc ataagttccg caggtcccag ctccatcggt tcctcagata tgccgacggc
5221 tccgacgatt ggtccaagta tttccagccg cagctccgcc agtccatgaa gtcttttggc
5281 tggctctgca actccgtgga agagatcgag acactcggct tctccatcct ccgcaactac
5341 accaagctgc cgatctgggg catcggccca cttattgctt ccccagtgca gcactcctcc
5401 tccgacaaca attcaacagg cgccgagttc gtgcagtggc tcagcctcaa agagccggac
5461 tccgtcctct acatctcctt cggctcccag aacacgatca gcccgacgca gatgatggaa
5521 ctcgctgctg gccttgagtc ctccgagaag ccattcctct gggtgatcag agccccgttc
5581 ggcttcgaca tcaacgaaga gatgcgccca gagtggctgc cagagggctt tgaggaacgc
5641 atgaaggtga agaaacaggg caagctcgtg tacaagctcg gcccgcagct tgagatcctc
5701 aaccatgaat ccatcggcgg ctttctcacc cactgcggat ggaacagcat ccttgagtct
5761 cttcgcgagg gcgttccgat gcttggatgg ccacttgctg ccgagcaggc ctacaacctc
5821 aagtacctcg aagatgagat gggcgtcgcg gttgagcttg ctagaggcct cgaaggcgag
5881 atctccaaag agaaggtcaa gcgcatcgtc gagatgatcc ttgagcgcaa cgagggctcc
5941 aaaggctggg agatgaagaa tcgcgccgtg gaaatgggca aaaagctcaa ggacgccgtg
6001 aacgaggaaa aagagctgaa gggctcctcc gtgaaggcga tcgacgattt cctcgacgcc
6061 gtcatgcagg ccaaacttga gccaagcctc cagtgatagt gaatatgaag atgaagatga
6121 aatatttggt gtgtcaaata aaaaggttgt gtgcttaagt ttgtgttttt ttcttggctt
6181 gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaacatc tcattataat
6241 gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctccagcat
6301 ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agacctaact
6361 gagtaagcta gcttgttcga gtattatggc attgggaaaa ctgtttttct tgtaccattt
6421 gttgtgcttg taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga
6481 aatggatgga gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt
6541 atttgttttt tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca
6601 ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg
6661 agtaaaacac tagatcccca aacaagcttg aaactgaagg cgggaaacga caatctgatc
6721 atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
6781 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg
6841 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
6901 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
6961 tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgga
7021 tccgctagag gatctcgacc tgcaagatcc cggggggcaa tgagatatga aaaagcctga
7081 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
7141 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatatag gagggcgtgg
7201 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
7261 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
7321 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
7381 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
7441 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
7501 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
7561 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
7621 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
7681 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
7741 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
7801 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
7861 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
7921 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
7981 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
8041 cgccgatagt ggaaaccgac gccccagcac tcgtccggga tcttggaggt gatgtaacat
8101 gatcacaagc tgatcccccg aatttccccg atcgttcaaa catttggcaa taaagtttct
8161 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
8221 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
8281 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
8341 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg atcccccctc
8401 gacagcttgc atgccagctt gggctgcagg tcgacgcgtc aattgatatc gatccaggcc
8461 tcccagcttt cgtccgtatc atcggtttcg acaacgttcg tcaagttcaa tgcatcagtt
8521 tcattgccca cacaccagaa tcctactaag tttgagtatt atggcattgg aaaagctgtt
8581 ttcttctatc atttgttctg cttgtaattt actgtgttct ttcagttttt gttttcggac
8641 atcaaaatgc aaatggatgg ataagagtta ataaatgata tggtcctttt gttcattctc
8701 aaattattat tatctgttgt ttttacttta atgggttgaa tttaagtaag aaaggaacta
8761 acagtgtgat attaaggtgc aatgttagac atataaaaca gtctttcacc tctctttggt
8821 tatgtcttga attggtttgt ttcttcactt atctgtgtaa tcaagtttac tatgagtcta
8881 tgatcaagta attatgcaat caagttaagt acagtatagg ctttttgtgt cgagggggta
8941 ccgagtcgag gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
9001 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
9061 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcggg taccgagctc
9121 gaattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
9181 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
9241 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
9301 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
9361 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
9421 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
9481 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
9541 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
9601 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
9661 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
9721 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
9781 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
9841 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
9901 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
9961 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
10021 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
10081 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
10141 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
10201 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
10261 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
10321 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
10381 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
10441 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
10501 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
10561 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
10621 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
10681 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
10741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
10801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
10861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
10921 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
10981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
11041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
11101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
11161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
11221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
11281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
11341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
11401 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
11461 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
11521 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
11581 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
11641 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
11701 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
11761 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
11821 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
11881 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
11941 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
12001 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
12061 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
12121 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
12181 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
12241 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
12301 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
12361 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
12421 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
12481 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
12541 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
12601 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
12661 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
12721 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
12781 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
12841 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
12901 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
12961 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
13021 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
13081 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
13141 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
13201 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
13261 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
13321 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
13381 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
13441 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
13501 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
13561 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
13621 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
13681 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
13741 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
13801 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
13861 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
13921 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
13981 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
14041 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
14101 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
14161 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
14221 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
14281 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
14341 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
14401 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
14461 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
14521 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
14581 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
14641 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
14701 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
14761 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
14821 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
14881 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
14941 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
15001 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
15061 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
15121 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
15181 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
15241 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
15301 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
15361 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
15421 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
15481 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
15541 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
15601 gatcaaagta ctttaaagta ctt
//
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