| 质粒名称: | sTR060 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 177370 |
| 存储实验室: | Jerome Bonnet, Jean-Loup Faulon |
|
载体骨架基本信息 |
|
| 载体名称: | Empty backbone |
| 载体类型: | 合成生物学；无细胞蛋白质合成 |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Trigger RNA，[PrT7-triggerRNA] |
|
文献引用方法 |
|
| 材料和方法部分: | sTR060 was a gift from Jerome Bonnet & Jean-Loup Faulon (Addgene plasmid # 177370 ; http://n2t.net/addgene:177370 ; RRID:Addgene_177370) |
| 参考文献部分: | Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts. Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M. ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448. 10.1021/acssynbio.1c00448 PubMed 35034449 |
买家导航
sTR060载体质粒基本信息
sTR060质粒图谱载体图谱和sTR060载体序列质粒序列多克隆位点信息
sTR060质粒载体简介
sTR060质粒序列载体序列
LOCUS sTR060 5242 bp DNA circular SYN 15-DEC-2025
DEFINITION Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the
toehold region in sTR056 (Addgene plasmid #177369) to restore
translational activity..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5242)
AUTHORS Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T,
Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL,
Beisel CL, Bonnet J, Kushwaha M
TITLE Differentially Optimized Cell-Free Buffer Enables Robust Expression
from Unprotected Linear DNA in Exonuclease-Deficient Extracts.
JOURNAL ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448.
PUBMED 35034449
REFERENCE 2 (bases 1 to 5242)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448."
COMMENT Sequence Label: sTR060
FEATURES Location/Qualifiers
source 1..5242
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(99..118)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 309..330
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 493..597
/gene="bla"
/label=AmpR promoter
CDS join(598..666,667..1458)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(816..835)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1629..2217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2118..2137
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2371..2388
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2403..2542
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2468..2487)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2628..2650
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2644..2835)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3644..4726)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 4688..4707
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(4727..4804)
/gene="lacI"
/label=lacI promoter
primer_bind 5010..5029
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 5113..5131
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
terminator 5170..5217
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
3301 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
3361 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
3421 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
3481 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
3541 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
3601 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
3661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
3721 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
3781 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
3841 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
3901 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
3961 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
4021 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
4081 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
4141 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
4201 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
4261 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
4321 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
4381 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
4441 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
4501 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
4561 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
4621 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
4681 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
4741 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
4801 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
4861 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
4921 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
4981 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
5041 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
5101 atcccgcgaa attaatacga ctcactatag gcatagcagg gataaacgag atagataaga
5161 taagacccgc tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
5221 aaaggaggaa ctatatccgg at
//
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