| 质粒名称: | pAAVLINK-3'-NSD1-FLAG-bGHpA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001927 |
| 产品用途: | 3' AAVLINK plasmid for NSD1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NSD1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-NSD1-FLAG-bGHpA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-NSD1-FLAG-bGHpA载体质粒基本信息
pAAVLINK-3'-NSD1-FLAG-bGHpA质粒图谱载体图谱和pAAVLINK-3'-NSD1-FLAG-bGHpA载体序列质粒序列多克隆位点信息
pAAVLINK-3'-NSD1-FLAG-bGHpA质粒载体简介
pAAVLINK-3'-NSD1-FLAG-bGHpA质粒序列载体序列
LOCUS pAAVLINK-3'-NSD1-FLAG-bGHpA 7431 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7431)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7431
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4371
/codon_start=1
/gene="NSD1"
/gene_synonym="ARA267; KMT3B; SOTOS; SOTOS1; STO"
/product="histone-lysine N-methyltransferase, H3
lysine-36specific isoform b"
/EC_number="2.1.1.357"
/label=NSD1
/note="isoform b is encoded by transcript variant
2;androgen receptor-associated coregulator 267;
histone-lysine N-methyltransferase, H3 lysine-36 and H4
lysine-20 specific; H3-K36-HMTase; H4-K20-HMTase; lysine
N-methyltransferase 3B; NR-binding SET domain-containing
protein; androgen receptor coactivator 267 kDa protein;
androgen receptor-associated protein of 267 kDa; nuclear
receptor-binding SET domain-containing protein 1; nuclear
receptor SET domain-containing protein 1; histone-lysine
N-methyltransferase, H3 lysine-36 specific"
/db_xref="GI:19923586"
/db_xref="CCDS:CCDS4412.1"
/db_xref="GeneID:64324"
/db_xref="HGNC:HGNC:14234"
/db_xref="MIM:606681"
/protein_id="NP_071900.2"
/translation="VDENSLISTKEEPPVLEREAPFLEGPLAQSELGGGHAELPQLTLS
VPVAPEVSPRPALESEELLVKTPGNYESKRQRKPTKKLLESNDLDPGFMPKKGDLGLSK
KCYEAGHLENGITESCATSYSKDFGGGTTKIFDKPRKRKRQRHAAAKMQCKKVKNDDSS
KEIPGSEGELMPHRTATSPKETVEEGVEHDPGMPASKKMQGERGGGAALKENVCQNCEK
LGELLLCEAQCCGAFHLECLGLTEMPRGKFICNECRTGIHTCFVCKQSGEDVKRCLLPL
CGKFYHEECVQKYPPTVMQNKGFRCSLHICITCHAANPANVSASKGRLMRCVRCPVAYH
ANDFCLAAGSKILASNSIICPNHFTPRRGCRNHEHVNVSWCFVCSEGGSLLCCDSCPAA
FHRECLNIDIPEGNWYCNDCKAGKKPHYREIVWVKVGRYRWWPAEICHPRAVPSNIDKM
RHDVGEFPVLFFGSNDYLWTHQARVFPYMEGDVSSKDKMGKGVDGTYKKALQEAAARFE
ELKAQKELRQLQEDRKNDKKPPPYKHIKVNRPIGRVQIFTADLSEIPRCNCKATDENPC
GIDSECINRMLLYECHPTVCPAGGRCQNQCFSKRQYPEVEIFRTLQRGWGLRTKTDIKK
GEFVNEYVGELIDEEECRARIRYAQEHDITNFYMLTLDKDRIIDAGPKGNYARFMNHCC
QPNCETQKWSVNGDTRVGLFALSDIKAGTELTFNYNLECLGNGKTVCKCGAPNCSGFLG
VRPKNQPIATEEKSKKFKKKQQGKRRTQGEITKEREDECFSCGDAGQLVSCKKPGCPKV
YHADCLNLTKRPAGKWECPWHQCDICGKEAASFCEMCPSSFCKQHREGMLFISKLDGRL
SCTEHDPCGPNPLEPGEIREYVPPPVPLPPGPSTHLAEQSTGMAAQAPKMSDKPPADTN
QMLSLSKKALAGTCQRPLLPERPLERTDSRPQPLDKVRDLAGSGTKSQSLVSSQRPLDR
PPAVAGPRPQLSDKPSPVTSPSSSPSVRSQPLERPLGTADPRLDKSIGAASPRPQSLEK
TSVPTGLRLPPPDRLLITSSPKPQTSDRPTDKPHASLSQRLPPPEKVLSAVVQTLVAKE
KALRPVDQNTQSKNRAALVMDLIDLTPRQKERAASPHQVTPQADEKMPVLESSSWPASK
GLGHMPRAVEKGCVSDPLQTSGKAAAPSEDPWQAVKSLTQARLLSQPPAKAFLYEPTTQ
ASGRASAGAEQTPGPLSQSPGLVKQAKQMVGGQQLPALAAKSGQSFRSLGKAPASLPTE
EKKLVTTEQSPWALGKASSRAGLWPIVAGQTLAQSCWSAGSTQTLAQTCWSLGRGQDPK
PEQNTLPALNQAPSSHKCAESEQK"
misc_feature 1428..1433
/label=SDS
misc_feature 2798..2803
/label=SDS
misc_feature 3043..3048
/label=SDS
misc_feature 3541..3546
/label=SDS
misc_feature 3907..3912
/label=SDS
CDS 4378..4443
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4447..4671
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4694..4834
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4694..4823
/label=AAV2 ITR
repeat_region 4695..4835
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4909..5364
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5646..5750
/gene="bla"
/label=AmpR promoter
CDS 5751..6611
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6782..7370
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtggacgaga attctttgat ttcaaccaaa
301 gaagagcctc cagttcttga aagggaggct ccgtttttgg agggcccctt ggctcagtca
361 gaacttggag gtggacatgc tgagttgccg cagctgacct tgtctgtgcc tgtggctccg
421 gaagtctctc cacggcctgc ccttgagtct gaggaattgc tagttaaaac gccaggaaat
481 tatgaaagta aacgtcaaag aaaaccaact aagaaacttc ttgaatccaa tgatttagac
541 cctggattta tgcccaagaa gggggacctt ggcctttcta aaaagtgcta tgaagctggt
601 cacctggaga atggcataac tgaatcttgt gccacatctt attcaaaaga ttttggtgga
661 ggcactacca agatatttga caagccaagg aagcgaaaac gacagaggca tgctgcagcc
721 aagatgcagt gtaaaaaagt gaaaaatgat gactcgtcaa aagagattcc aggctcagag
781 ggagaactaa tgcctcacag gacggccaca agccccaagg agactgttga ggaaggtgta
841 gaacacgatc ccgggatgcc tgcctctaaa aaaatgcagg gtgaacgcgg tggaggagct
901 gcactcaagg agaatgtctg tcagaattgt gaaaaattgg gtgagctgct gttatgtgag
961 gctcagtgct gtggggcttt ccacctggag tgccttggat tgactgagat gccaagagga
1021 aaatttatct gcaatgaatg tcgcacagga atccatacct gttttgtatg taagcagagt
1081 ggggaagatg ttaaaaggtg ccttctaccc ttgtgtggaa agttttacca tgaagagtgt
1141 gtccagaagt acccacccac tgttatgcag aacaagggct tccggtgctc cctccacatc
1201 tgtataacct gtcatgctgc taatccagcc aatgtttctg catctaaagg tcggttgatg
1261 cgctgtgtcc gctgtcctgt ggcataccac gccaatgact tttgcctggc tgctgggtca
1321 aagatccttg catctaatag tatcatctgc cctaatcact ttacccctag gcggggctgc
1381 cgaaatcatg agcatgttaa tgttagctgg tgctttgtgt gctcagaagg aggcagcctt
1441 ctgtgctgtg attcttgccc tgctgctttt catcgtgaat gcctgaacat tgatatccct
1501 gaaggaaact ggtattgcaa tgactgtaaa gcaggcaaaa agccacacta cagggagatt
1561 gtctgggtaa aagttggacg atacaggtgg tggccagctg agatctgcca tcctcgagct
1621 gttccttcca acattgataa gatgagacat gatgtgggag agttcccagt cctctttttt
1681 ggatctaatg actatttgtg gactcaccag gcccgagtct tcccttacat ggagggtgac
1741 gtgagcagca aggataagat gggcaaagga gtggatggga catataaaaa agctcttcag
1801 gaagctgcag caaggtttga ggaattaaag gcccaaaaag agctaagaca gctgcaggaa
1861 gaccgaaaga atgacaagaa gccaccacct tataaacata taaaggtaaa ccgtcctatt
1921 ggcagggtac agatcttcac tgcagactta tctgaaatac cccgttgcaa ctgtaaagct
1981 actgatgaga acccctgtgg gatagactct gaatgcatca accgcatgct gctctatgag
2041 tgccacccca cagtgtgtcc tgccggaggg cgctgtcaaa accagtgctt ttccaagcgc
2101 caatatccag aggttgaaat tttccgcaca ttacagcggg gttggggtct acggacaaaa
2161 acagatatta aaaagggtga atttgtgaat gagtatgtgg gtgagcttat agatgaagaa
2221 gaatgcagag ctcgaattcg ctatgctcaa gaacatgata tcactaattt ctatatgctc
2281 accctagaca aagaccgaat cattgatgct ggtcccaaag gaaactatgc tcggttcatg
2341 aatcattgct gccagcccaa ctgtgaaaca cagaagtggt ctgtgaatgg agatacccgt
2401 gtaggccttt ttgcactaag tgacattaaa gcaggcactg aacttacctt caactacaac
2461 ctagaatgtc ttgggaatgg aaagactgtt tgcaaatgtg gagccccgaa ctgcagtggc
2521 ttcttgggtg taaggccaaa gaatcaaccc attgccacgg aagaaaagtc aaagaaattc
2581 aagaagaagc aacagggaaa gcgcaggacc cagggtgaaa tcacaaagga gcgagaagat
2641 gagtgtttta gttgtgggga tgctggccag ctcgtctcct gcaagaaacc aggctgccca
2701 aaagtttacc acgcagactg tctcaatctg accaagcgac cagcagggaa atgggaatgt
2761 ccgtggcatc agtgtgacat ctgcgggaag gaagcagcct ccttctgtga gatgtgcccc
2821 agctcctttt gtaagcagca tcgagaaggg atgcttttca tttccaaact ggatgggcgt
2881 ctgtcttgta ctgagcatga cccctgtggg cccaatcctc tggaacctgg ggagatccgt
2941 gagtatgtgc ctcccccagt accgctgcct ccagggccaa gcactcacct ggcagagcaa
3001 tcaacaggaa tggctgctca ggcacccaaa atgtcagata aacctcctgc tgacaccaac
3061 cagatgctgt cgctctccaa aaaagctctg gcagggactt gtcagaggcc actgctacct
3121 gaaagacctc ttgagagaac tgactccagg ccccagcctt tagataaggt cagagacctc
3181 gctgggtcag ggaccaaatc ccaatccttg gtttccagcc agaggccact ggacaggcca
3241 ccagcagtgg caggaccaag accccagcta agcgacaaac cctctccagt gaccagccca
3301 agctcctcac cctcagtcag gtcccaacca ctggaaagac ctctggggac ggctgaccca
3361 aggctggata aatccatagg tgctgccagc ccaaggcccc agtcactgga gaaaacctca
3421 gttcccactg gcctgagact tccgccgcca gacagactgc tcattactag cagtcccaaa
3481 ccccagactt cagacaggcc tactgacaaa ccccatgcct ctttgtccca gagactccca
3541 cctcctgaga aagtactatc agctgtggtc cagacccttg tagctaaaga aaaagcactg
3601 aggcctgtgg accagaatac tcagtcaaaa aatagagctg ctttggtgat ggatctcata
3661 gacctaactc ctcgccagaa ggagcgggca gcttcacctc atcaggtcac accacaggct
3721 gatgagaaga tgccagtgtt ggagtcaagt tcatggcctg ccagcaaagg tctggggcat
3781 atgccgagag ctgttgagaa aggctgtgtg tcagatcctc ttcagacatc tgggaaagca
3841 gcagcccctt cagaggaccc ctggcaagct gttaaatcac tcacccaggc cagacttctt
3901 tctcagcctc ctgccaaggc ctttttatat gagccaacaa ctcaggcctc aggaagagct
3961 tctgcagggg ctgagcagac cccagggcct cttagccaat ccccgggcct ggtgaagcag
4021 gcgaagcaga tggtcggagg ccagcaacta cctgcacttg ccgccaagag tgggcaatct
4081 tttaggtctc tcgggaaggc cccagcctcc ctccccactg aagaaaagaa gttggtaacc
4141 acagagcaaa gtccctgggc cctgggaaaa gcctcatcac gggcagggct ctggcccata
4201 gtggctggac agacactggc acagtcttgc tggtctgctg ggagcacaca gacattggca
4261 cagacttgct ggtctcttgg aagagggcaa gaccccaaac cagagcaaaa tacacttcca
4321 gctcttaacc aggctccttc cagtcacaag tgtgcagaat cagaacagaa gtgtacagac
4381 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
4441 aagtaactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
4501 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
4561 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
4621 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcacgtgcgg
4681 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
4741 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
4801 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
4861 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
4921 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
4981 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
5041 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
5101 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
5161 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
5221 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
5281 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
5341 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
5401 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
5461 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
5521 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
5581 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
5641 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
5701 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
5761 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
5821 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
5881 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
5941 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
6001 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
6061 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
6121 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
6181 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
6241 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
6301 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
6361 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
6421 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
6481 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
6541 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
6601 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
6661 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
6721 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
6781 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
6841 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
6901 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
6961 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
7021 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
7081 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
7141 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
7201 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
7261 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
7321 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
7381 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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