| 质粒名称: | pDJ656 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001155 |
| 产品用途: | mutiplexed signaling recording for STAT3 and NFκB |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | STAT3-responsive, NFkB-responsive |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mChilada-iPAK4, emiRFP670-Ipak4 |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Fluorescent protein-based ticker tapes for multiplexed recordings of transcriptional histories in single cells in culture and in vivo
Wang R., Jiang J., Li Z., Liu T., Wang Y., Xie M., Piatkevich K.D.
Preprint
|
pDJ656
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDJ656载体质粒基本信息
pDJ656质粒图谱载体图谱和pDJ656载体序列质粒序列多克隆位点信息
pDJ656质粒载体简介
pDJ656质粒序列载体序列
LOCUS pDJ656 10187 bp DNA circular SYN 10-MAR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10187)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10187
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 78..99
/label=oYW269
/note="LA-NheI-F"
misc_feature 196..226
/label=SleepingBeauty-ITR_Lo
/label=nonstandard type: ITR
misc_feature 392..423
/label=SleepingBeauty-ITR_Li
/label=nonstandard type: ITR
primer_bind 451..498
/label=oYW336
primer_bind 460..476
/label=oYW181
/note="P_hCMV-F"
primer_bind complement(460..476)
/label=oYW179
/note="ITR PCR F"
misc_feature 477..1580
/label=EF-1a
/note="/vntifkey=21"
primer_bind 477..498
/label=oYW338
/note="LA-NruI-PEF1a PCR-F"
promoter 493..1671
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
primer_bind 496..510
/label=oYW43
/note="LA-MluI-hEF1a PCR F"
primer_bind 496..510
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 604..627
/label=oDJ223-1
primer_bind 605..627
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 605..624
/label=oDJ8-1
primer_bind complement(611..630)
/label=EF1a-R
primer_bind complement(671..690)
/label=oYW45
/note="hEF1a mutant TOP motif LA-R"
primer_bind 673..713
/label=oYW46
/note="hEF1a mutant TOP motif RA-F"
intron 724..1662
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 937..956
/label=oYW361
/note="PEF1a mid seq primer"
primer_bind 1076..1093
/label=oYW47
/note="hEF1a-Seq-F"
primer_bind 1438..1459
/label=oYW285
/note="PEF1a-F for pYW136/137"
primer_bind complement(1551..1560)
/label=oYW346
primer_bind 1619..1639
/label=pEF-F/EF-1aFwd
primer_bind complement(1640..1659)
/label=oYW184
/note="P_hEF1a PCR R"
primer_bind complement(1643..1697)
/label=oYW337
primer_bind complement(1643..1672)
/label=oYW348
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1672)
/label=oYW354
/note="RA-PEF1a PCR R"
primer_bind complement(1643..1671)
/label=oYW339
/note="RA-NheI-PEF1a PCR R"
primer_bind 1643..1659
/label=oYW180
/note="Puro PCR R"
primer_bind 1667..1676
/label=oDJ587-4
primer_bind 1670..1707
/label=oYW333
/note="LA-AgeI-PacI-EcoRV-IRES PCR-F"
misc_feature 1730..2303
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1776..1795)
/label=pIRES2-EGFP.P3
primer_bind complement(1825..1844)
/label=IRES-R
primer_bind 2301..2312
/label=oDJ199-3
primer_bind complement(2316..2361)
/label=oYW335
/note="RA-SalI-IRES PCR R"
primer_bind complement(2316..2350)
/label=oYW344
primer_bind complement(2316..2350)
/label=oYW345
primer_bind complement(2316..2335)
/label=oYW334
/note="IRES PCR R"
primer_bind 2336..2373
/label=oYW193
/note="LA-PuroR-F"
primer_bind 2351..2373
/label=oYW180
/note="Puro PCR R"
CDS 2353..2952
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2838..2891)
/label=oYS113
primer_bind complement(2937..2952)
/label=oYW194
/note="PuroR-R"
primer_bind 2982..3001
/label=oDJ653-3
primer_bind 2982..3001
/label=oDJ656-3
primer_bind complement(2995..3012)
/label=BGH/pcDNA3.1R
primer_bind complement(2995..3012)
/label=pcDNA3.1-R(BGH-R)
primer_bind 2998..3017
/label=oYW188
/note="bGH poly A"
polyA_signal 3001..3225
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(3005..3022)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(3067..3085)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(3067..3085)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(3070..3097)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(3070..3093)
/label=oYW302
/note="bGH pA R"
primer_bind 3070..3089
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 3128..3147
/label=BGH-polyA-R
primer_bind 3208..3254
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(3208..3231)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(3208..3225)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(3208..3225)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(3209..3226)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind 3226..3254
/label=oYW189
/note="LA-P_CMV-F"
primer_bind 3245..3262
/label=oYW90
/note="LA-AgeI-PBS-PCMV F"
primer_bind 3246..3271
/label=oYW355
/note="LA-NruI-PEF1a PCR-F"
primer_bind 3254..3270
/label=oYW326
primer_bind 3274..3317
/label=oDJ656-1
primer_bind 3274..3293
/label=oDJ223-1
primer_bind 3274..3292
/label=oYW278
/note="LA-NotI-P_GFAP-F"
primer_bind 3274..3292
/label=oYW181
/note="P_hCMV-F"
primer_bind 3294..3317
/label=oDJ584-1
misc_feature 3326..3426
/label=4x STAT binding
misc_feature 3326..3340
/label=STAT3 binding motif
misc_feature 3350..3369
/label=STAT3 binding motif
misc_feature 3375..3394
/label=STAT3 binding motif
misc_feature 3378..3386
/label=TTCNNNgAA
misc_feature 3400..3419
/label=STAT3 binding motif
misc_feature 3427..3471
/label=P_CMVmin
promoter 3432..3470
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3515..3533
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 3527..3546
/label=oYW346
primer_bind complement(3536..3555)
/label=oDJ584-2
primer_bind 3536..3555
/label=oDJ584-3
primer_bind 3541..3582
/label=tet-Chilada-F
primer_bind 3562..3582
/label=XbaI-mGL-F
regulatory 3562..3571
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3568..4275
/codon_start=1
/label=mChilada
/translation="MVSKGEEDNMAIIKEYMRFKVHMEGSVNGHEFEIEGEGEGRPFEG
TQTAKLKVTKGGPLPFAWHILPPQFQYGSKAYVKHPADIPDYFKLSFPEGFTWEREMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGNTASTERMYPEDGALK
GETKWRLKLKDGGHYEAEVKTTYKAKKPVQLPGAYNVDRKLKITYHNEDYTIVEQYERA
EARHSTGGMDELYK"
primer_bind 3572..3588
/label=oYS113
primer_bind complement(4260..4301)
/label=INKA-mGreenLantern-R
primer_bind 4275..4297
/label=oDJ587-5
CDS 4288..4401
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 4408..5349
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(4501..4519)
/label=PAK4-R
primer_bind complement(5332..5367)
/label=oDJ656-6
primer_bind complement(5332..5362)
/label=oDJ656-2
primer_bind complement(5332..5353)
/label=HindIII-PAK4-R
primer_bind complement(5332..5353)
/label=HindIII-PAK4-Ra
primer_bind complement(5332..5353)
/label=HindIII-PAK4-R`b
primer_bind complement(5333..5352)
/label=oDJ580-2
primer_bind 5343..5373
/label=oDJ656-3
primer_bind 5352..5373
/label=oDJ653-3
primer_bind complement(5367..5384)
/label=BGH/pcDNA3.1R
primer_bind complement(5367..5384)
/label=pcDNA3.1-R(BGH-R)
terminator 5370..5597
/label=bGH PA
primer_bind 5370..5389
/label=oYW188
/note="bGH poly A"
primer_bind complement(5377..5394)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(5439..5457)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(5439..5457)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(5442..5469)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(5442..5465)
/label=oYW302
/note="bGH pA R"
primer_bind 5442..5461
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 5500..5519
/label=BGH-polyA-R
primer_bind complement(5580..5631)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind 5580..5598
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(5580..5598)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(5580..5597)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(5581..5598)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind complement(5597..5629)
/label=oDJ656-4
primer_bind complement(5597..5619)
/label=oDJ653-4
primer_bind 5610..5640
/label=oDJ656-5
primer_bind 5620..5640
/label=oDJ587-1
primer_bind 5621..5645
/label=oYW269
/note="LA-NheI-F"
misc_feature 5646..5655
/label=nfkb motif
misc_feature 5656..5665
/label=nfkb motif
misc_feature 5666..5675
/label=nfkb motif
misc_feature 5676..5685
/label=nfkb motif
promoter 5698..5729
/label=minP
/note="minimal TATA-box promoter with low basal activity"
misc_feature 5699..5729
/label=Minimal promoter
misc_feature 5705..5711
/label=TATATAA
primer_bind complement(5733..5754)
/label=oDJ587-2
primer_bind 5742..5774
/label=oDJ587-3
regulatory 5755..5764
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 5761..6690
/codon_start=1
/product="bright monomeric near-infrared fluorescent
protein engineered from a bacterial phytochrome, with an
emission maximum of 670 nm (Shcherbakova et al., 2016)"
/label=emiRFP670
/label=ORF frame 1
/translation="MAEGSVARQPDLLTCEHEEIHLAGSIQPHGALLVVSEHDHRVIQA
SANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTEYCGLM
HRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTGYDRVM
VYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVTYQPVP
LEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLVVCHHY
LPRFIRFELRAICKRLAERIATRITALES"
primer_bind complement(6671..6713)
/label=0117-miRFP67-R
primer_bind 6675..6685
/label=oYW194
/note="PuroR-R"
primer_bind complement(6694..6724)
/label=oDJ587-4
primer_bind 6705..6736
/label=oDJ587-5
primer_bind complement(6714..6740)
/label=INKA-mGreenLantern-R
CDS 6714
/codon_start=1
/label=mGreenLantern
/translation=""
CDS 6727..6840
/codon_start=1
/label=INKA-box
/translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
CDS 6847..7788
/codon_start=1
/label=Pak4cat
/translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
primer_bind complement(6940..6958)
/label=PAK4-R
primer_bind complement(7771..7811)
/label=oDJ656-6
primer_bind complement(7771..7806)
/label=oDJ656-2
primer_bind complement(7771..7791)
/label=HindIII-PAK4-R
primer_bind complement(7771..7791)
/label=HindIII-PAK4-Ra
primer_bind complement(7771..7791)
/label=HindIII-PAK4-R`b
primer_bind complement(7772..7791)
/label=oDJ580-2
primer_bind 7782..7817
/label=oDJ656-3
primer_bind 7797..7817
/label=oDJ653-3
primer_bind complement(7811..7828)
/label=BGH/pcDNA3.1R
primer_bind complement(7811..7828)
/label=pcDNA3.1-R(BGH-R)
primer_bind 7814..7833
/label=oYW188
/note="bGH poly A"
polyA_signal 7817..8041
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(7821..7838)
/label=oYW190
/note="BGH poly A R"
primer_bind complement(7883..7901)
/label=oYW303
/note="PolyA tail-bGH pA"
primer_bind complement(7883..7901)
/label=oYW57
/note="bGH seq primer R"
primer_bind complement(7886..7913)
/label=oYW300
/note="PolyA tail-bGH pA"
primer_bind complement(7886..7909)
/label=oYW302
/note="bGH pA R"
primer_bind 7886..7905
/label=W2F0627-P099-D03
/note="Sequencing primer"
primer_bind 7944..7963
/label=BGH-polyA-R
primer_bind 8024..8042
/label=oYW203
/note="LA-P_CMV-F"
primer_bind complement(8024..8042)
/label=oYW202
/note="BGH polyA-R"
primer_bind complement(8024..8041)
/label=oYW56
/note="LA-BamHI-PPT-MluI-bGH pA PCR"
primer_bind complement(8024..8041)
/label=oYW53
/note="LA-BamHI-bGH pA"
primer_bind complement(8025..8042)
/label=oYW282
/note="Arm-PCR pYW134-R"
primer_bind complement(8041..8063)
/label=oDJ653-4
primer_bind complement(8041..8063)
/label=oDJ656-4
primer_bind 8052..8068
/label=oYW183
/note="P_hEF1a PCR F"
primer_bind 8053..8074
/label=oYW197
/note="ITR PCR F1"
primer_bind complement(8084..8099)
/label=oYW336
misc_feature complement(8125..8153)
/label=SleepingBeauty-ITR_Ri
/label=nonstandard type: ITR
misc_feature 8319..8350
/label=SleepingBeauty-ITR-Ro
/label=nonstandard type: ITR
primer_bind complement(8493..8512)
/label=oYW207
/note="pLL3.7 PCR R"
primer_bind complement(8494..8519)
/label=oYW301
/note="PolyA tail-pLL3.7 3'LTR"
rep_origin complement(8538..9126)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8677..8696)
/label=W5CF0703-P099-D09
/note="Sequencing primer"
CDS complement(9297..10157)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9314..9333)
/label=W4CF0701-P099-E08
/note="Sequencing primer"
primer_bind 9492..9503
/label=oDJ587-3
primer_bind complement(9978..9997)
/label=W3CF0630-P099-D08
/note="Sequencing primer"
primer_bind complement(10034..10053)
/label=W3CF0630-P099-A08
/note="Sequencing primer"
primer_bind complement(10034..10053)
/label=W3CF0630-P099-F07
/note="Sequencing primer"
primer_bind 10128..10147
/label=oYW24
/note="Forward seq primer of AmpR"
promoter complement(join(10158..10187,1..75))
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
61 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc
121 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
181 attggatccc tatacagttg aagtcggaag tttacataca cttaagttgg agtcattaaa
241 actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt ggcaagtcag
301 ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt tacagacaga
361 ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt acatacacta
421 agttcgactc ctctgcagaa tgcggcgatg tttcggtaag gggtccgcta gaattcgaaa
481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
1021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
1081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
1141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
1201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
1261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
1321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
1381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
1441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
1501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
1561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
1621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aaccggttta
1681 attaagatat ctgcatctag ggcggccaat tccgcccctc tcccccccgc ccctctccct
1741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
1801 atatgtgatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
1861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
1921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
1981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
2041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
2101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
2161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
2221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
2281 tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac cttccgtcga
2341 cgccaccatg ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
2401 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
2461 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
2521 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
2581 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
2641 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
2701 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
2761 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
2821 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
2881 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
2941 cccggtgcct gattcgaagg cctgtcgtgg gggatcaatt cgctcgctga tcagcctcga
3001 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
3061 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
3121 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
3181 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcctag gagaatctgc
3241 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcatgctc
3301 taggaagatc tccacgcgtc tcgagatttc ccgtaaatcg tcgagtggac atttcccgta
3361 aatcgtcgag tggacatttc ccgtaaatcg tcgagtggac atttcccgta aatcgtcgag
3421 ctgcagtcga gggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa
3481 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
3541 aagctgttcg aagcggaatt cgccaccatg gtgagcaagg gcgaggagga caacatggct
3601 atcatcaagg aatacatgcg tttcaaggtt catatggaag gttctgttaa cggtcatgaa
3661 ttcgaaatcg aaggtgaagg tgaaggtcgt cctttcgaag gtacccagac cgctaagctg
3721 aaggttacca agggtggtcc tctgcctttc gcttggcata tcctgcctcc tcagttccag
3781 tacggttcta aggcttacgt taagcatcct gctgacatcc ctgactactt caagctgtct
3841 ttccctgaag gtttcacctg ggaacgtgaa atgaacttcg aagacggtgg tgttgttacc
3901 gttacccagg actcttctct gcaggacggt gaattcatct acaaggttaa gctgcgtggt
3961 accaacttcc cttctgacgg tcctgttatg cagaagaaga ccatgggtaa caccgcttct
4021 accgaacgta tgtaccctga agacggtgct ctgaagggtg aaaccaagtg gcgtctgaag
4081 ctgaaggacg gtggtcatta cgaagctgaa gttaagacca cctacaaggc taagaagcct
4141 gttcagctgc ctggtgctta caacgttgac cgtaagctga agatcaccta ccataacgaa
4201 gactacacca tcgttgaaca gtacgaacgt gctgaagctc gtcattctac cggtggcatg
4261 gacgagctgt acaagtcagg tggatccgaa gcagaggact ggacagcagc cctactgaac
4321 aggggtcgca gtcgccagcc cctggtacta ggggacaatt gctttgctga cttggtgcac
4381 aactggatgg agctgcctga ggaattcccc gccgcccctg ctgttcctgg gccccctggc
4441 ccccgctcac cacagcggga gccacagcga gtatcccatg agcagttccg ggctgccctg
4501 cagctggtgg tggacccagg cgacccccgc tcctacctgg acaacttcat caagattggc
4561 gagggctcca cgggcatcgt gtgcatcgcc accgtgcgca gctcgggcaa gctggtggcc
4621 gtcaagaaga tggacctgcg caagcagcag aggcgcgagc tgctcttcaa cgaggtggta
4681 atcatgaggg actaccagca cgagaatgtg gtggagatgt acaacagcta cctggtgggg
4741 gacgagctct gggtggtcat ggagttcctg gaaggaggcg ccctcaccga catcgtcacc
4801 cacaccagga tgaacgagga gcagatcgca gccgtgtgcc ttgcagtgct gcaggccctg
4861 tcggtgctcc acgcccaggg cgtcatccac cgggacatca agagcgactc gatcctgctg
4921 acccatgatg gcagggtgaa gctgtcagac tttgggttct gcgcccaggt gagcaaggaa
4981 gtgccccgaa ggaagtcgct ggtcggcacg ccctactgga tggccccaga gctcatctcc
5041 cgccttccct acgggccaga ggtagacatc tggtcgctgg ggataatggt gattgagatg
5101 gtggacggag agccccccta cttcaacgag ccacccctca aagccatgaa gatgattcgg
5161 gacaacctgc caccccgact gaagaacctg cacaaggtgt cgccatccct gaagggcttc
5221 ctggaccgcc tgctggtgcg agaccctgcc cagcgggcca cggcagccga gctgctgaag
5281 cacccattcc tggccaaggc agggccgcct gccagcatcg tgcccctcat gcgccagaac
5341 cgcaccagat gaagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
5401 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
5461 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
5521 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
5581 tgcggtgggc tctatggctt ctgaggcgga aagaaccagg gtaccgagct cttacgcgtg
5641 ctagcgggaa tttccgggaa tttccgggaa tttccgggaa tttccagatc tagactctag
5701 agggtatata atggaagctc gaattccagc ttggcattcc ggtactgttg gtaagccacc
5761 atggccgagg gcagcgtggc cagacagccc gacctgctga cctgcgaaca tgaagagatc
5821 cacctcgccg gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga acatgatcat
5881 cgcgtcatcc aggccagcgc caacgccgcg gaatttctga atctcggaag cgtactcggc
5941 gttccgctcg ccgagatcga cggcgatctg ttgatcaaga tcctgccgca tctcgatccc
6001 accgccgaag gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc tacggagtac
6061 tgcggtctga tgcatcggcc tccggaaggc gggctgatca tcgaactcga acgtgccggc
6121 ccgtcgatcg atctgtcagg cacgctggcg ccggcgctgg agcggatccg cacggcgggt
6181 tcactgcgcg cgctgtgcga tgacaccgtg ctgctgtttc agcagtgcac cggctacgac
6241 cgggtgatgg tgtatcgttt cgatgagcaa ggccacggcc tggtattctc cgagtgccat
6301 gtgcctgggc tcgaatccta tttcggcaac cgctatccgt cgtcgactgt cccgcagatg
6361 gcgcggcagc tgtacgtgcg gcagcgcgtc cgcgtgctgg tcgacgtcac ctatcagccg
6421 gtgccgctgg agccgcggct gtcgccgctg accgggcgcg atctcgacat gtcgggctgc
6481 ttcctgcgct cgatgtcgcc gtgccatctg cagttcctga aggacatggg cgtgcgcgcc
6541 accctggcgg tgtcgctggt ggtcggcggc aagctgtggg gcctggttgt ctgtcaccat
6601 tatctgccgc gcttcatccg tttcgagctg cgggcgatct gcaaacggct cgccgaaagg
6661 atcgcgacgc ggatcaccgc gcttgagagc cgaccggttc acgtgggaag tgggtcaggt
6721 ggatccgaag cagaggactg gacagcagcc ctactgaaca ggggtcgcag tcgccagccc
6781 ctggtactag gggacaattg ctttgctgac ttggtgcaca actggatgga gctgcctgag
6841 gaattccccg ccgcccctgc tgttcctggg ccccctggcc cccgctcacc acagcgggag
6901 ccacagcgag tatcccatga gcagttccgg gctgccctgc agctggtggt ggacccaggc
6961 gacccccgct cctacctgga caacttcatc aagattggcg agggctccac gggcatcgtg
7021 tgcatcgcca ccgtgcgcag ctcgggcaag ctggtggccg tcaagaagat ggacctgcgc
7081 aagcagcaga ggcgcgagct gctcttcaac gaggtggtaa tcatgaggga ctaccagcac
7141 gagaatgtgg tggagatgta caacagctac ctggtggggg acgagctctg ggtggtcatg
7201 gagttcctgg aaggaggcgc cctcaccgac atcgtcaccc acaccaggat gaacgaggag
7261 cagatcgcag ccgtgtgcct tgcagtgctg caggccctgt cggtgctcca cgcccagggc
7321 gtcatccacc gggacatcaa gagcgactcg atcctgctga cccatgatgg cagggtgaag
7381 ctgtcagact ttgggttctg cgcccaggtg agcaaggaag tgccccgaag gaagtcgctg
7441 gtcggcacgc cctactggat ggccccagag ctcatctccc gccttcccta cgggccagag
7501 gtagacatct ggtcgctggg gataatggtg attgagatgg tggacggaga gcccccctac
7561 ttcaacgagc cacccctcaa agccatgaag atgattcggg acaacctgcc accccgactg
7621 aagaacctgc acaaggtgtc gccatccctg aagggcttcc tggaccgcct gctggtgcga
7681 gaccctgccc agcgggccac ggcagccgag ctgctgaagc acccattcct ggccaaggca
7741 gggccgcctg ccagcatcgt gcccctcatg cgccagaacc gcaccagatg atctagagct
7801 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
7861 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
7921 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
7981 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
8041 gcttctgagg cggaaagaac cagctgggcc tactagctac tcgggacccc ttaccgaaac
8101 atcgccgcat tctgcagagg agtcgagtgt atgtaaactt ctgacccact gggaatgtga
8161 tgaaagaaat aaaagctgaa atgaatcatt ctctctacta ttattctgat atttcacatt
8221 cttaaaataa agtggtgatc ctaactgacc taagacaggg aatttttact aggattaaat
8281 gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg taaacttccg
8341 acttcaactg tatagggatc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
8401 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
8461 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
8521 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
8581 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
8641 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
8701 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
8761 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
8821 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
8881 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
8941 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
9001 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
9061 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
9121 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
9181 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
9241 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
9301 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
9361 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
9421 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
9481 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
9541 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
9601 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
9661 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
9721 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
9781 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
9841 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
9901 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
9961 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
10021 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
10081 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
10141 aaatgttgaa tactcatact cttccttttt caatattatt gaagcat
//
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