| 质粒名称: | pSLIK-Venus |
|---|---|
| 出品公司: | Iain Fraser |
| 目录编号: | 25734 |
| 存储实验室: | Iain Fraser |
|
载体骨架基本信息 |
|
| 载体名称: | pSLIK |
| 载体出品公司: | Iain Fraser |
| 完整质粒大小: | 13170bp |
| 载体修饰: | DB3.1 (ccdB survival) |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Venus |
| 载体抗性: | Chloramphenicol and Ampicillin, 25 & 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 克隆方法: | Gateway Cloning |
| 5’克隆位点: | attR1(未破坏) |
| 3’克隆位点: | attR2 (未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | EXFP-R |
|
文献引用方法 |
|
| 材料和方法部分: | pSLIK-Venus was a gift from Iain Fraser (Addgene plasmid # 25734 ; http://n2t.net/addgene:25734 ; RRID:Addgene_25734) |
| 参考文献部分: | A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression. Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID. Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64. 10.1073/pnas.0606179103 PubMed 16945906 |
买家导航
pSLIK-Venus载体质粒基本信息
pSLIK-Venus质粒图谱载体图谱和pSLIK-Venus载体序列质粒序列多克隆位点信息
pSLIK-Venus质粒载体简介
pSLIK-Venus质粒序列载体序列
LOCUS pSLIK-Venus 13170 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for Tet-based inducible shRNA or cDNA expression,
constitutive Venus fluorescent protein coexpression..
ACCESSION .
VERSION .
KEYWORDS pSLIK-Venus
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13170)
AUTHORS Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI,
Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
TITLE A single lentiviral vector platform for microRNA-based conditional
RNA interference and coordinated transgene expression.
JOURNAL Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.
PUBMED 16945906
REFERENCE 2 (bases 1 to 13170)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13170
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2614..2738
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2763..2793
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2847..3506
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2913..2932)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3848..4153
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4032..4051
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(4194..4318)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 4368..5579
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 5416..5435
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 5704..6450
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 6488..7062
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6655..6672)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6882..6901
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 7090..7809
/codon_start=1
/product="yellow fluorescent protein (YFP) with fast and
efficient maturation (Nagai et al., 2002)"
/label=Venus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(7135..7156)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(7396..7415)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7743..7764
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 7858..8446
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7911..7931)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8329..8340)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(8449..8465)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8450..8466)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8975..9155
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9181..9198)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9187..9411
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9457..9885
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9544..9563)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9754..9775
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9894..9914)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9899..10228
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10079..10214
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10141..10160
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 10276..10323
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10342..10716
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10846..10967
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10883..10902)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10937..10956
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11016..11032)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11016..11032)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11029..11051)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11040..11056
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11064..11094)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11109..11130
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11247..11264)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11418..12006)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11498..11517)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12177..13037)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12800..12819
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13038..13142)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agctgaacga
2641 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
2701 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
2761 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga
2821 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
2881 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
2941 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
3001 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
3061 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
3121 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
3181 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
3241 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
3301 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
3361 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
3421 atcatgccgt ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
3481 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
3541 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
3601 gaagtatgtc aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga
3661 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
3721 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
3781 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac
3841 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
3901 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
3961 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
4021 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
4081 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
4141 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
4201 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
4261 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
4321 ataacatctc gagccataaa gatggttaat taacccaccc aagatctggc ctccgcgccg
4381 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
4441 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
4501 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
4561 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
4621 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
4681 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
4741 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
4801 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
4861 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
4921 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
4981 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
5041 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
5101 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
5161 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
5221 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
5281 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
5341 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
5401 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
5461 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
5521 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
5581 aagcttggta ccgagctcgg atctccaccc cgtaccggtc ctgcaggatt taaatggcgc
5641 gccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc agtcgaattc
5701 accatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
5761 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
5821 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
5881 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
5941 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
6001 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
6061 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
6121 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
6181 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
6241 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
6301 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
6361 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
6421 gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc gcactagagg
6481 aattccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
6541 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
6601 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
6661 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
6721 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
6781 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
6841 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
6901 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggattc tgatctgggg
6961 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
7021 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttaccg gtacgcgtga
7081 tgatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
7141 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
7201 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
7261 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
7321 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
7381 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
7441 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
7501 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
7561 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
7621 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
7681 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
7741 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
7801 tacaagtaag aattgttgtt gtttagtccc tcccaattcg atatcaagct tatcgataat
7861 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
7921 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
7981 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
8041 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
8101 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
8161 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
8221 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
8281 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
8341 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
8401 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
8461 cctcgatcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
8521 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
8581 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
8641 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
8701 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
8761 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
8821 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
8881 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
8941 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
9001 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
9061 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
9121 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
9181 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
9241 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
9301 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
9361 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
9421 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
9481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
9541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
9601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
9661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
9721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
9781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
9841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
9901 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
9961 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
10021 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
10081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
10141 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
10201 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
10261 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
10321 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
10381 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
10441 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
10501 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
10561 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
10621 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
10681 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
10741 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
10801 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
10861 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
10921 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
10981 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
11041 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
11101 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
11161 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
11221 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
11281 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
11341 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
11401 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
11461 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
11521 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
11581 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
11641 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
11701 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
11761 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
11821 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
11881 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
11941 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
12001 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
12061 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
12121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
12181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
12241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
12301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
12361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
12421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
12481 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
12541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
12601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
12661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
12721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
12781 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
12841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
12901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
12961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
13021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
13081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
13141 cgcacatttc cccgaaaagt gccacctgac
//
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