| 质粒名称: | PABE-1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115622 |
| 存储实验室: | Caixia Gao |
| 载体骨架基本信息 | |
| 载体名称: | pJIT163 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NLS-wtTadA-TadA7.10-nCas9-NLS |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PABE-1 was a gift from Caixia Gao (Addgene plasmid # 115622 ; http://n2t.net/addgene:115622 ; RRID:Addgene_115622) |
| 参考文献部分: | Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion. Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C. Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z. 10.1186/s13059-018-1443-z PubMed 29807545 |
买家导航
PABE-1载体 PABE1质粒基本信息
PABE-1质粒图谱 PABE1载体图谱和PABE-1载体序列 PABE1质粒序列多克隆位点信息
PABE-1质粒 PABE1载体简介
PABE-1质粒序列 PABE1载体序列
LOCUS PABE-1 10342 bp ds-DNA circular SYN 10-JAN-2019
DEFINITION Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION .
VERSION .
KEYWORDS PABE-1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10342)
AUTHORS Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
TITLE Expanded base editing in rice and wheat using a Cas9-adenosine
deaminase fusion.
JOURNAL Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
PUBMED 29807545
REFERENCE 2 (bases 1 to 10342)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 10, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10342
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 814..834
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2041..6141
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
RBS 6054..6062
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 6142..6189
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
polyA_signal 6247..6423
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
primer_bind complement(6977..6993)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6977..6993)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6990..7012)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7001..7017
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7025..7055)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7070..7091
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7208..7225)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7379..7967)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7459..7478)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8138..8998)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8761..8780
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8999..9103)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aatcctggga tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt
61 cgttgcatag ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt
121 gtcgggtcat cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc
181 ggtcgttcta gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat
241 tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg
301 aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag
361 atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc
421 tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta
481 tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg
541 ataggtatac atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat
601 ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa
661 ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt
721 tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc
781 ctgttgtttg gtgttacttc tgcaaagctt atgccaaaaa agaagagaaa ggtttcaggc
841 ggctcctccg aggtggagtt ctctcacgag tattggatga ggcacgctct tacacttgct
901 aagagagctt gggacgaaag agaagtgcca gttggcgccg ttcttgtgca taataatagg
961 gtgatcggcg agggttggaa tagaccaatt ggaaggcatg atccaacagc tcacgcagag
1021 attatggctc tcagacaagg cggcctcgtt atgcagaact acaggctcat tgacgctaca
1081 ctctacgtga cactcgaacc ttgcgttatg tgcgccggag ctatgattca ttctaggatt
1141 ggcagggtcg tgtttggagc tagggacgct aaaacaggag ccgccggatc tcttatggac
1201 gtgttgcatc atccaggcat gaaccatagg gtggagatta cagagggcat tcttgcagac
1261 gagtgcgctg ctcttctttc cgatttcttc aggatgagaa ggcaggagat taaggcccag
1321 aagaaggctc agtcttctac agatagcgga ggatcttccg gaggatctag cggctccgag
1381 acaccaggaa catccgaaag cgctacacca gaatctagcg gaggctcttc cggaggatct
1441 tctgaagtgg agttctccca cgagtattgg atgaggcacg ctcttacact tgctaaaagg
1501 gctagggacg aaagggaagt tccagttgga gctgttctcg tgctcaataa cagggtgatt
1561 ggcgagggtt ggaatagagc cattggactc catgatccaa cagctcacgc agagattatg
1621 gctcttagac aaggcggcct cgttatgcag aattacagac tcatcgacgc cacactctac
1681 gttaccttcg aaccttgcgt tatgtgcgcc ggagctatga tccattctag gattggcagg
1741 gtcgtgttcg gcgttagaaa cgctaagaca ggagctgcag gctctcttat ggacgttctt
1801 cattacccag gcatgaatca tagagtggag atcacagaag gcattcttgc agacgagtgc
1861 gcagctctcc tttgctattt cttcaggatg ccgaggcaag ttttcaacgc tcagaagaag
1921 gcccagtctt ctacagattc cggcggatct tctggaggat ctagcggctc cgagacacca
1981 ggaacatccg aatccgctac accagagtct tctggaggat ctagcggagg atctcttaag
2041 gacaagaagt actcgatcgg cctcgccatt gggactaact ctgttggctg ggccgtgatc
2101 accgacgagt acaaggtgcc ctcaaagaag ttcaaggtcc tgggcaacac cgatcggcat
2161 tccatcaaga agaatctcat tggcgctctc ctgttcgaca gcggcgagac ggctgaggct
2221 acgcggctca agcgcaccgc ccgcaggcgg tacacgcgca ggaagaatcg catctgctac
2281 ctgcaggaga ttttctccaa cgagatggcg aaggttgacg attctttctt ccacaggctg
2341 gaggagtcat tcctcgtgga ggaggataag aagcacgagc ggcatccaat cttcggcaac
2401 attgtcgacg aggttgccta ccacgagaag taccctacga tctaccatct gcggaagaag
2461 ctcgtggact ccacagataa ggcggacctc cgcctgatct acctcgctct ggcccacatg
2521 attaagttca ggggccattt cctgatcgag ggggatctca acccggacaa tagcgatgtt
2581 gacaagctgt tcatccagct cgtgcagacg tacaaccagc tcttcgagga gaaccccatt
2641 aatgcgtcag gcgtcgacgc gaaggctatc ctgtccgcta ggctctcgaa gtctcggcgc
2701 ctcgagaacc tgatcgccca gctgccgggc gagaagaaga acggcctgtt cgggaatctc
2761 attgcgctca gcctggggct cacgcccaac ttcaagtcga atttcgatct cgctgaggac
2821 gccaagctgc agctctccaa ggacacatac gacgatgacc tggataacct cctggcccag
2881 atcggcgatc agtacgcgga cctgttcctc gctgccaaga atctgtcgga cgccatcctc
2941 ctgtctgata ttctcagggt gaacaccgag attacgaagg ctccgctctc agcctccatg
3001 atcaagcgct acgacgagca ccatcaggat ctgaccctcc tgaaggcgct ggtcaggcag
3061 cagctccccg agaagtacaa ggagatcttc ttcgatcagt cgaagaacgg ctacgctggg
3121 tacattgacg gcggggcctc tcaggaggag ttctacaagt tcatcaagcc gattctggag
3181 aagatggacg gcacggagga gctgctggtg aagctcaatc gcgaggacct cctgaggaag
3241 cagcggacat tcgataacgg cagcatccca caccagattc atctcgggga gctgcacgct
3301 atcctgagga ggcaggagga cttctaccct ttcctcaagg ataaccgcga gaagatcgag
3361 aagattctga ctttcaggat cccgtactac gtcggcccac tcgctagggg caactcccgc
3421 ttcgcttgga tgacccgcaa gtcagaggag acgatcacgc cgtggaactt cgaggaggtg
3481 gtcgacaagg gcgctagcgc tcagtcgttc atcgagagga tgacgaattt cgacaagaac
3541 ctgccaaatg agaaggtgct ccctaagcac tcgctcctgt acgagtactt cacagtctac
3601 aacgagctga ctaaggtgaa gtatgtgacc gagggcatga ggaagccggc tttcctgtct
3661 ggggagcaga agaaggccat cgtggacctc ctgttcaaga ccaaccggaa ggtcacggtt
3721 aagcagctca aggaggacta cttcaagaag attgagtgct tcgattcggt cgagatctct
3781 ggcgttgagg accgcttcaa cgcctccctg gggacctacc acgatctcct gaagatcatt
3841 aaggataagg acttcctgga caacgaggag aatgaggata tcctcgagga cattgtgctg
3901 acactcactc tgttcgagga ccgggagatg atcgaggagc gcctgaagac ttacgcccat
3961 ctcttcgatg acaaggtcat gaagcagctc aagaggagga ggtacaccgg ctgggggagg
4021 ctgagcagga agctcatcaa cggcattcgg gacaagcagt ccgggaagac gatcctcgac
4081 ttcctgaaga gcgatggctt cgcgaaccgc aatttcatgc agctgattca cgatgacagc
4141 ctcacattca aggaggatat ccagaaggct caggtgagcg gccaggggga ctcgctgcac
4201 gagcatatcg cgaacctcgc tggctcgcca gctatcaaga aggggattct gcagaccgtg
4261 aaggttgtgg acgagctggt gaaggtcatg ggcaggcaca agcctgagaa catcgtcatt
4321 gagatggccc gggagaatca gaccacgcag aagggccaga agaactcacg cgagaggatg
4381 aagaggatcg aggagggcat taaggagctg gggtcccaga tcctcaagga gcacccggtg
4441 gagaacacgc agctgcagaa tgagaagctc tacctgtact acctccagaa tggccgcgat
4501 atgtatgtgg accaggagct ggatattaac aggctcagcg attacgacgt cgatcatatc
4561 gttccacagt cattcctgaa ggatgactcc attgacaaca aggtcctcac caggtcggac
4621 aagaaccggg gcaagtctga taatgttcct tcagaggagg tcgttaagaa gatgaagaac
4681 tactggcgcc agctcctgaa tgccaagctg atcacgcagc ggaagttcga taacctcaca
4741 aaggctgaga ggggcgggct ctctgagctg gacaaggcgg gcttcatcaa gaggcagctg
4801 gtcgagacac ggcagatcac taagcacgtt gcgcagattc tcgactcacg gatgaacact
4861 aagtacgatg agaatgacaa gctgatccgc gaggtgaagg tcatcaccct gaagtcaaag
4921 ctcgtctccg acttcaggaa ggatttccag ttctacaagg ttcgggagat caacaattac
4981 caccatgccc atgacgcgta cctgaacgcg gtggtcggca cagctctgat caagaagtac
5041 ccaaagctcg agagcgagtt cgtgtacggg gactacaagg tttacgatgt gaggaagatg
5101 atcgccaagt cggagcagga gattggcaag gctaccgcca agtacttctt ctactctaac
5161 attatgaatt tcttcaagac agagatcact ctggccaatg gcgagatccg gaagcgcccc
5221 ctcatcgaga cgaacggcga gacgggggag atcgtgtggg acaagggcag ggatttcgcg
5281 accgtcagga aggttctctc catgccacaa gtgaatatcg tcaagaagac agaggtccag
5341 actggcgggt tctctaagga gtcaattctg cctaagcgga acagcgacaa gctcatcgcc
5401 cgcaagaagg actgggatcc gaagaagtac ggcgggttcg acagccccac tgtggcctac
5461 tcggtcctgg ttgtggcgaa ggttgagaag ggcaagtcca agaagctcaa gagcgtgaag
5521 gagctgctgg ggatcacgat tatggagcgc tccagcttcg agaagaaccc gatcgatttc
5581 ctggaggcga agggctacaa ggaggtgaag aaggacctga tcattaagct ccccaagtac
5641 tcactcttcg agctggagaa cggcaggaag cggatgctgg cttccgctgg cgagctgcag
5701 aaggggaacg agctggctct gccgtccaag tatgtgaact tcctctacct ggcctcccac
5761 tacgagaagc tcaagggcag ccccgaggac aacgagcaga agcagctgtt cgtcgagcag
5821 cacaagcatt acctcgacga gatcattgag cagatttccg agttctccaa gcgcgtgatc
5881 ctggccgacg cgaatctgga taaggtcctc tccgcgtaca acaagcaccg cgacaagcca
5941 atcagggagc aggctgagaa tatcattcat ctcttcaccc tgacgaacct cggcgcccct
6001 gctgctttca agtacttcga cacaactatc gatcgcaaga ggtacacaag cactaaggag
6061 gtcctggacg cgaccctcat ccaccagtcg attaccggcc tctacgagac gcgcatcgac
6121 ctgtctcagc tcgggggcga caagcggcca gcggcgacga agaaggcggg gcaggcgaag
6181 aagaagaagt aggaattcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct
6241 ctctattttc tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt
6301 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
6361 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
6421 atctcctaaa gtccctatag atctttgtcg tgaatataaa ccagacacga gacgactaaa
6481 cctggagccc agacgccgtt cgaagctaga agtaccgctt aggcaggagg ccgttaggga
6541 aaagatgcta aggcagggtt ggttacgttg actcccccgt aggtttggtt taaatatgat
6601 gaagtggacg gaaggaagga ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg
6661 taagaagatg gaaatttgat agaggtacgc tactatactt atactatacg ctaagggaat
6721 gcttgtattt ataccctata ccccctaata accccttatc aatttaagaa ataatccgca
6781 taagcccccg cttaaaaatt ggtatcagag ccatgaatag gtctatgacc aaaactcaag
6841 aggataaaac ctcaccaaaa tacgaaagag ttcttaactc taaagataaa agatctttca
6901 agatcaaaac tagttccctc acaccggtga cggggatcgc atgcgatatc tcgagatcta
6961 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
7021 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
7081 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
7141 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
7201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
7261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
7321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
7381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
7441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
7501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
7561 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
7621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
7681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
7741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
7801 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
7861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
8041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
8101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
8161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
8221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
8281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
8341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
8401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
8461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
8521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
8581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
8641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
8701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
8761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
8821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
8881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
8941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
9001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
9061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
9121 tgccacctgc cagtgccaag ctaattcgag ctcggtacct gacccggtcg tgcccctctc
9181 tagagataat gagcattgca tgtctaagtt ataaaaaatt accacatatt ttttttgtca
9241 cacttgtttg aagtgcagtt tatctatctt tatacatata tttaaacttt actctacgaa
9301 taatataatc tatagtacta caataatatc agtgttttag agaatcatat aaatgaacag
9361 ttagacatgg tctaaaggac aattgagtat tttgacaaca ggactctaca gttttatctt
9421 tttagtgtgc atgtgttctc cttttttttt gcaaatagct tcacctatat aatacttcat
9481 ccattttatt agtacatcca tttagggttt agggttaatg gtttttatag actaattttt
9541 ttagtacatc tattttattc tattttagcc tctaaattaa gaaaactaaa actctatttt
9601 agttttttta tttaataatt tagatataaa atagaataaa ataaagtgac taaaaattaa
9661 acaaataccc tttaagaaat taaaaaaact aaggaaacat ttttcttgtt tcgagtagat
9721 aatgccagcc tgttaaacgc cgtcgacgag tctaacggac accaaccagc gaaccagcag
9781 cgtcgcgtcg ggccaagcga agcagacggc acggcatctc tgtcgctgcc tctggacccc
9841 tctcgatcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg
9901 cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca
9961 ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
10021 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
10081 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
10141 gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
10201 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
10261 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
10321 ttgccagtgt ttctctttgg gg
//
