pCDH_cgeMTS-GFP-LplA3M

LOCUS       pCDH_cgeMTS-GFP-LplA3M            8265 bp DNA     circular SYN 03-JUL-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             213..284
                     /label=cgeSOD2
     CDS             285..998
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1029..2048
                     /label=LplA3M
     misc_feature    1395..1401
                     /label=N121A D122A
     misc_feature    1431..1433
                     /label=K133A
     misc_feature    2055..2642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2526..2537)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2715..2948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3020..3141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      3181..3316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3349..3365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3373..3389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3397..3427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3442..3463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3751..4339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4510..5370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5371..5475)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5949..5965
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5980..6206
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6207..6387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6434..6559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7052..7285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7470..7514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7781..7897
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7920..8223
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8224..8265,1..162)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
       61 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      121 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
      181 attctagacc gctagcgcta ccggtcgcca ccatgctatg tcgtgctgcc tgctccgctg
      241 gccgccgact cgctcccgct gccactgccg ctggatcccg gcatgtgagc aagggcgagg
      301 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      361 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      421 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      481 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      541 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      601 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      661 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      721 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      781 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      841 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
      901 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      961 ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga tctcgagctc
     1021 aagcttccat gggctccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
     1081 acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc
     1141 tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg aaagagtgta
     1201 atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg
     1261 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
     1321 ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg
     1381 aagcgtccgg acgtgccgcc ctggtggtga aaaccgtcga aggcgaccgc gcggtctcag
     1441 gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg ctactcaatg
     1501 ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
     1561 gcattacgtc ggtacgttcc cgcgtgacca acctcaccga gctgttgccg gggatcaccc
     1621 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcgcgtgg
     1681 aagcggaaat catctccccg aacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
     1741 cccgccagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
     1801 atgaacgctt tacctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
     1861 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccggac
     1921 gactgcaagg ctgcctgtac cgcgcagata tgctgcaaca ggagtgcgaa gcgctgttgg
     1981 ttgacttccc ggaacaggaa aaagagctac gggagttatc ggcatggatg gcgggggctg
     2041 taaggtaggg atccaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     2461 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     2521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     2581 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc
     2641 gccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2701 agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc
     2761 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2821 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2881 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2941 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3001 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3061 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3121 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     3181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3301 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt
     3361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3721 gaaccttaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     5521 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     5581 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     5641 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     5701 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     5761 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     5821 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     5881 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     5941 acgacgttgt aaaacgacgg ccagtgccaa gctgacgcgt gtagtcttat gcaatactct
     6001 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6061 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6121 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6181 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6241 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6301 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6361 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6421 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6481 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6541 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     6601 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     6661 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     6721 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     6781 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     6841 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     6901 cactgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     6961 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7021 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7081 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7141 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7201 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7261 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7321 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7381 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7441 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7501 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7561 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     7621 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     7681 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     7741 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
     7801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     7861 aagaattaca aaaacaaatt acaaaattca aaattttatc gatactagtt ggagttccgc
     7921 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7981 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8041 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8101 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8161 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8221 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcgg
//