| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pCR2.1-TOPO |
| 质粒类型: | 细菌表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | TOPO克隆 |
| 启动子: | Plac |
| 载体大小: | 3900 bp |
| 5' 测序引物及序列: | M13 |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
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pCR2.1-TOPO载体 pCR2.1TOPO质粒基本信息
pCR2.1-TOPO质粒图谱 pCR2.1TOPO载体图谱和pCR2.1-TOPO载体序列 pCR2.1TOPO质粒序列多克隆位点信息
pCR2.1-TOPO质粒 pCR2.1TOPO载体简介
pCR2.1-TOPO质粒序列 pCR2.1TOPO载体序列
LOCUS pCR2.1-TOPO 3931 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Parental bacterial expression vector for TOPO(R) TA cloning of PCR
products.
ACCESSION .
VERSION .
KEYWORDS pCR2.1-TOPO
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3931)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Friday, February 21, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3931
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
protein_bind 107..128
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 205..221
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 217..570
/codon_start=1
/gene="lacZ (fragment)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLVPSSDPLVTAASVLEFALKGEFCRYPSHWRPLEHASR
GPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR
SLNGEWTRPVAAH"
promoter complement(365..383)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(390..406)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 547..975
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS 1319..2113
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2131..2991
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3162..3750
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttaagggc
301 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat
361 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
541 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
961 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
1021 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
1081 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
1141 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
1201 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
1261 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
1321 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
1381 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
1441 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
1501 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
1561 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
1621 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
1681 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
1741 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
1801 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
1861 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
1921 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
1981 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
2041 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
2101 cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
2161 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
2221 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
2281 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
2341 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
2401 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
2461 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
2521 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
2581 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
2641 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
2701 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
2761 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
2821 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
2881 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
2941 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
3001 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
3061 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
3121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
3181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
3241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
3301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
3361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
3421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
3481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
3541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
3601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
3661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
3721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
3781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
3841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
3901 cgcagcgagt cagtgagcga ggaagcggaa g
//
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