| 质粒名称: | px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002340 |
| 产品用途: | A human codon-optimized ISYmu1 TnpB and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Dh5a |
|
插入基因信息 |
|
| 插入基因名称: | Nuclease, mCherry |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry载体质粒基本信息
px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry质粒图谱载体图谱和px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry质粒载体简介
px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-ISYmu1 gRNA(Bbs1)-CAG-ISYmu1-P2A-mCherry 6227 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6227)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6227
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 651..899
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
enhancer 1060..1345
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1347..1623
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
promoter 1468..1487
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 1624..1851
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 1863..1872
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1869..3068
/codon_start=1
/label=ISYmu1_humanized
/translation="MAPKKKRKVGIHGVPAATSLQHKAYEYRIYPDKKQETLIAKTIGS
SRYVYNHFLELWNKEYEETGKGLTYYACSKLLTKLKRDPETVWLCEVDKFSLQNSLRNL
SDAFSRFFKGQNEHPQFKSKKSPRQSYTTQYTNNNIAVSGNCLKLPKLGLVKFADSREM
KGRILNATVRRKSSGKFFVSILCEEEICELPKTDSSVGIDLGIIDFAVMSDGSRHDNNH
FTRQMEERLRREQRKLARRALAAEKRGISLSEARNYQKQRRKVARLYEKVANQRKEYLN
KLSTEIVKNHDIICIEDLNVKGMMRNHKLAKSISDVSWTSLVSKLQYKASWYGKEVIRI
SRWFPSSQICSECGHKDRKKPLHVREWTCPVCHAHHDRDVNAARNILAEGLRIRALTPG
S"
CDS 1875..1895
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 2428..2433
/label=SDS
misc_feature 2872..2877
/label=SDS
CDS 3069..3116
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 3126..3182
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3183..3893
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 3199..3204
/label=SDS
misc_feature 3631..3636
/label=SDS
polyA_signal 3924..4131
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4140..4280
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4140..4269
/label=AAV2 ITR
rep_origin 4355..4810
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5092..5196
/gene="bla"
/label=AmpR promoter
CDS 5197..6057
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gagggcctat
661 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
721 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
781 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
841 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
901 aaccgcagga attgcggggg tagcttggta aacaagagaa acctctgccg gcaaagaaat
961 aagccggtaa gtatgctctg ttcccaagaa tctcgtgact ttagtcatga gagtttcaaa
1021 tgtcttcctg ggacgaagac aattttttct agaggtaccc gttacataac ttacggtaaa
1081 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
1141 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
1201 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
1261 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
1321 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
1381 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
1441 agcgatgggg gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
1501 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
1561 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
1621 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
1681 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
1741 cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg
1801 gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg
1861 gtgccaccat ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagcca
1921 ctagtctgca gcacaaggcc tacgagtaca gaatctaccc cgacaagaag caggagaccc
1981 tgatcgccaa gaccatcggc agctccaggt acgtgtacaa ccacttcctg gagctgtgga
2041 acaaagagta cgaggagacc ggaaaggggc tgacctacta cgcctgcagc aaactgctga
2101 ccaagctgaa aagggacccc gagacagtgt ggctgtgcga agtggacaag tttagcctgc
2161 agaactccct gagaaacctg agcgacgcct tcagcaggtt cttcaagggc cagaacgagc
2221 acccccagtt caagagcaaa aagagccccc ggcagagcta caccacccag tataccaaca
2281 acaacatcgc cgtgagcgga aactgtctga agctgcccaa gctgggactg gtgaaattcg
2341 ccgacagcag agagatgaag ggaagaatcc tgaacgccac agtgagaaga aagagcagcg
2401 gaaagttctt cgtgagcatc ctgtgcgagg aggagatctg cgagctgcct aagaccgata
2461 gcagcgtggg aatcgacctg ggaatcattg acttcgccgt gatgagcgac ggatcaagac
2521 acgacaacaa ccacttcacc cggcagatgg aggagaggct gagaagagaa cagagaaaac
2581 tggccagaag agccctggcc gccgaaaaac ggggaatcag tctgagcgag gccagaaact
2641 accagaaaca gagaagaaag gtggccagac tgtacgagaa ggtggccaac cagagaaaag
2701 aatacctgaa taaactgagc acagagatcg tgaaaaacca cgacatcatc tgcatcgagg
2761 acctgaacgt gaagggaatg atgagaaacc acaaactggc taagagcatc agcgacgtga
2821 gctggaccag cctggtgtct aagctgcagt acaaagccag ctggtacggg aaggaggtga
2881 tcagaatcag cagatggttc cccagcagcc agatctgtag cgagtgcgga cacaaggaca
2941 gaaaaaagcc cctgcacgtg agagagtgga cctgccccgt gtgccacgcc caccacgaca
3001 gagacgtgaa cgccgccaga aacatcctgg ccgagggcct gagaatcaga gccctgaccc
3061 ccggcagcaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggaa
3121 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
3181 ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
3241 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
3301 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
3361 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
3421 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
3481 gcgtgatgaa cttcgatgac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
3541 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
3601 taatgcagaa gaaaactatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
3661 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
3721 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
3781 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
3841 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag taagaattcc
3901 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
3961 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
4021 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
4081 gcaggacagc aagggggagg attgggaaga gaatagcagg catgctgggg agcggccgca
4141 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
4201 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
4261 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
4321 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
4381 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
4441 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
4501 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
4561 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
4621 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
4681 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
4741 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
4801 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
4861 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
4921 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
4981 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
5041 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
5101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
5161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
5221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
5281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
5341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
5401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
5461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
5521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
5581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
5641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
5701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
5761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
5821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
5881 attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc agcactgggg
5941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
6001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
6061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
6121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
6181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
