| 质粒名称: | pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002201 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting EMX1 gene NickingRNA and pegRNA, harboring dual selection markers of puroR and RFP. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | EMX1-nicking-sgRNA-pegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1载体质粒基本信息
pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1质粒载体简介
pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-EMX1-Nicking/PegRNA-PGK-puroR-mRFP1 5684 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5684)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5684
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 21..55
/label=OL2640
primer_bind 24..40
/label=OL1149
misc_feature 41..115
/label=hCtRNA
primer_bind 56..79
/label=OL464
primer_bind complement(98..115)
/label=OL465
primer_bind complement(101..135)
/label=OL2646
primer_bind complement(101..115)
/label=OL2648
primer_bind 116..164
/label=OL2724
primer_bind 116..151
/label=OL2647
misc_feature 116..135
/label=EMX1-nickingRNA
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..176
/label=OL462
primer_bind complement(194..230)
/label=OL463
misc_feature 212..286
/label=hCtRNA
primer_bind 212..226
/label=OL2640
primer_bind 227..250
/label=OL464
primer_bind complement(269..286)
/label=OL465
primer_bind complement(272..306)
/label=OL2648
primer_bind complement(272..286)
/label=OL2646
primer_bind 287..310
/label=OL2649
misc_feature 287..306
/label=EMX1 spacer
primer_bind 291..306
/label=OL2650
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 307..347
/label=OL462
primer_bind 307..335
/label=OL2724
primer_bind 307..322
/label=OL2647
primer_bind complement(365..382)
/label=OL463
misc_feature 383..404
/label=RTT
primer_bind complement(399..441)
/label=OL2725
primer_bind complement(402..441)
/label=OL2650
primer_bind 402..417
/label=OL2648
misc_feature 405..417
/label=PBS
primer_bind complement(424..440)
/label=OL1148
misc_feature 461..578
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 627..1137
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1056..1074
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1159..1755
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1159..1178)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1655..1675
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1726..1780
/label=OL901
primer_bind complement(1741..1763)
/label=OL384
primer_bind 1748..1779
/label=OL379
CDS 1756..1812
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 1756..1812
/label=OL866
primer_bind complement(1756..1812)
/label=OL867
primer_bind 1793..1836
/label=OL902
primer_bind complement(1793..1820)
/label=OL966
RBS 1795..1802
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1813..2490
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(2468..2515)
/label=OL903
primer_bind 2489..2507
/label=OL965
polyA_signal 2637..2758
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2648..2667)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2702..2721
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(3006..3023)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3177..3765)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3257..3276)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3936..4796)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4559..4578
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4797..4901)
/gene="bla"
/label=AmpR promoter
rep_origin 4928..5383
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5015..5034)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5225..5246
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5514..5562
/note="synthetic polyadenylation signal"
misc_feature 5576..5667
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5616..5635
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctctccc
121 cattggcctg cttcggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctgagt ccgagcagaa
301 gaagaagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gcgttgatgt gatgggagcc gttcttcttc tgctcggttt
421 ttttctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
481 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
541 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
601 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
661 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
721 cctgggactc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
781 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
841 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
901 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
961 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
1021 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
1081 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
1141 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
1201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
1261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
1321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
1381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
1441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
1501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
1561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
1621 ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta cgagcggctc
1681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
1741 cgcaagcccg gtgccgctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
1801 aaccctggac ctatggcgag tagcgaagac gttatcaaag agttcatgcg tttcaaagtt
1861 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
1921 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
1981 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
2041 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
2101 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
2161 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
2221 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
2281 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
2341 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
2401 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
2461 gctgaaggtc gtcactccac cggtgcttaa cgcccgcccc acgacccgca gcgcccgacc
2521 gaaaggagcg cacgacccca tgcatcggta cctttaagac caatgactta caaggcagct
2581 gtagatctta gccactttct agagtcgggg cggccggccg cttcgagcag acatgataag
2641 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
2701 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2761 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
2821 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
2881 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
2941 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
3001 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
3061 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
3121 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
3181 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
3241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
3301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
3361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
3421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
3481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
3541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
3601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
3661 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
3721 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
3781 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
3841 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
3901 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
3961 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
4021 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgggac
4081 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
4141 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
4201 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
4261 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
4321 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
4381 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
4441 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
4501 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
4561 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
4621 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
4681 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
4741 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
4801 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
4861 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
4921 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4981 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
5041 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
5101 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
5161 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
5221 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
5281 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
5341 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg
5401 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag
5461 ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa
5521 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat
5581 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
5641 caagtgcagg tgccagaaca tttctctatc gataggtacc gatt
//
