pLV-EF1a-mCherry-MinD-IRES-Puro

LOCUS       pLV-EF1a-mCherry-MinD-IRES-Puro               10253 bp ds-DNA     circular SYN 26-JAN-2024
DEFINITION  Expression of mCherry-MinD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-EF1a-mCherry-MinD-IRES-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
  TITLE     A programmable reaction-diffusion system for spatiotemporal cell 
            signaling circuit design.
  JOURNAL   Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 
            10.1016/j.cell.2023.12.007.
  PUBMED    38181787
REFERENCE   2  (bases 1 to 10253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2024 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             106..739
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    786..911
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1408..1641
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1826..1870
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2133..2248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2326..2537
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2538..3476
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3433..3453
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3492..3501
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3498..4205
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3643..3661)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3911..3930
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(5296..5313)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5523..5542
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5704..6303
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5704..5723)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6200..6220
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6327..6915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6380..6400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6798..6809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7122..7755
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7883..7899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7883..7899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7896..7918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7907..7923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7931..7961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7976..7997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8114..8131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8285..8873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8365..8384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9044..9904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9667..9686
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9905..10009)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10057..10191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10094..10113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10148..10167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
       61 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
      121 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
      181 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
      241 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
      301 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
      361 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
      421 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
      481 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
      541 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      601 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      661 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      721 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      781 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      841 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      901 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      961 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1021 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1081 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1141 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1201 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     1261 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1321 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1381 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1441 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1501 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1561 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1621 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1681 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1741 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1801 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1861 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1921 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1981 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2041 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2101 tcgattagtg aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt
     2161 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2221 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2281 tttatcgatg aggccctttc gtcttcactc gaggtgcccg tcagtgggca gagcgcacat
     2341 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2401 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2461 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2521 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2581 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2641 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2701 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2761 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2821 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2881 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     2941 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3001 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3061 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3121 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3181 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3241 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3301 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3361 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3421 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3481 cgtgaggatc cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     3541 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     3601 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     3661 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     3721 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     3781 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     3841 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     3901 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     3961 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     4021 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     4081 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     4141 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     4201 acaaggacag cgccggaagt gctggctctg cgggtaccat ggcccgcata atagttgtga
     4261 cttcaggtaa gggcggagtt ggaaaaacca catccagcgc agccatagct accggcctcg
     4321 cacagaaagg caagaaaact gtggtcatag attttgatat tgggctcagg aacctggacc
     4381 tgataatggg ctgcgaacgg cgcgttgtct acgattttgt caatgtcatc caaggcgacg
     4441 cgactcttaa tcaggcactt ataaaagata aacgaaccga aaacttgtat attcttcctg
     4501 cctcacaaac gcgggacaaa gacgcgctca ccagggaggg tgtcgcaaaa gttttggatg
     4561 acttgaaagc gatggacttt gagttcatag tttgtgactc tcccgccggt atagagacag
     4621 gggcacttat ggcactgtac ttcgcagatg aagccataat cacaactaat ccggaggtgt
     4681 ctagtgtgcg agattcagac cgaatactgg ggatactcgc cagcaaatca cggagggcag
     4741 aaaacgggga ggagccaatt aaggagcatc tcctgcttac ccgatataac cccggtcgag
     4801 tatctcgggg agatatgctt tcaatggaag atgtcctcga gattctgagg ataaaactgg
     4861 taggcgttat cccggaagac cagtccgtcc tccgcgcatc taatcagggg gagcctgtca
     4921 tacttgacat aaatgcggat gctgggaaag cttatgccga tacggtagaa cggctcctcg
     4981 gggaagaacg gcccttccgc ttcatagagg aggaaaagaa gggattcctt aaacggctgt
     5041 tcgggggata agcggccgct ctagagtcga cgggccgcgg taacaattgt taactaactt
     5101 aagctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     5161 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     5221 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     5281 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     5341 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     5401 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     5461 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     5521 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     5581 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     5641 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgataat
     5701 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5761 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5821 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5881 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5941 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6001 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6061 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6121 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6181 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6241 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6301 tgacccgggc ggggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     6361 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6421 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6481 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6541 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6601 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6661 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6721 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6781 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6841 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6901 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat
     6961 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     7021 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     7081 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc
     7141 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7201 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     7261 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     7321 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     7381 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7441 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7501 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     7561 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7621 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7681 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7741 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7801 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta
     7861 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7921 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7981 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8041 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     8101 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8161 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8221 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8281 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8341 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8401 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8461 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8521 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8581 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8641 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8701 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8761 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8821 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8881 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8941 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     9001 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     9061 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9121 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9181 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9241 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9301 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9361 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9421 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9481 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9541 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9601 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9661 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9721 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9781 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9841 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9901 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9961 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    10021 gaaaagtgcc acctgacgtc gacggatcga gagatcaact tgtttattgc agcttataat
    10081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10201 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cac
//