| 质粒名称: | pHybrid-Electra2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000203 |
| 产品用途: | Dual-expression vector. Expresses Electra2 in bacterial cells and co-expresses Electra2 with mScarlet in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra2, mScarlet |
| 物种来源: | Synthetic, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
买家导航
pHybrid-Electra2载体质粒基本信息
pHybrid-Electra2质粒图谱载体图谱和pHybrid-Electra2载体序列质粒序列多克隆位点信息
pHybrid-Electra2质粒载体简介
pHybrid-Electra2质粒序列载体序列
LOCUS pHybrid-Electra2 5609 bp DNA circular SYN 31-MAY-2023
DEFINITION 73.Electra2TSN20211022-0571-00003_1023-ban2_A10_A10_i7.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5609)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5609
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 101..480
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 481..684
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 717..835
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
CDS 876..893
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
regulatory 924..933
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 930..1643
/codon_start=1
/label=Electra2
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTD
IALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAV
AKYSNLGGGMDELFK"
terminator 1664..1750
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 1774..1895
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1936..2146
/label=SV40 promoter
/note="SV40 early promoter"
promoter 1958..2154
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 2005..2140
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 2155..2164
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2161..2856
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
polyA_signal 2861..2908
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 2988..3443
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3775..3879
/gene="bla"
/label=AmpR promoter
CDS 3880..4740
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4911..5499
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
61 tacatttata ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt
121 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
181 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
241 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
301 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
361 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
421 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
481 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
541 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
601 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
661 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcactag aagcttcacc
721 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
781 tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaacccg
841 tttttggcta acaggaggaa ttaaccatgg ggagccacca tcaccatcac catgctagcc
901 tcgagaattc acgcgtggta ccagccacca tggtgtctaa gggcgaagag ctgatcgagg
961 aaaatatgcg tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca
1021 caggtgaagg agaaggcaga ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg
1081 agggaggacc cctgccattt gcctttgaca ttcttgccac gtcgttcctg ttcggcagca
1141 agacctttat caagtacccg gccgacatcc ctgatttctt cgaacagtcc tttcctgagg
1201 gctttacttg ggaaagagtt acgagatacg aagatggtgg agtagtcacc gtcacgcagg
1261 acaccagcct tgaggatggt gggctcgtct acaacgtcaa ggtcagaggg gtaaactttc
1321 actccaaggg tcccgtgatg cagaagaaga ccgagggttg ggagcctttc acagagatga
1381 tgtatccagc agatggtggt ctgagaggat ataccgacat cgcactgaaa gttgatggtg
1441 gtggccatct gcacgccaac atcgtgacaa cttacaggtc aaaaaagacc gtcgggaaca
1501 tcaagatgcc cggtgtccat gccgttgatt accgcctgga aaggatcgaa gagagtgaca
1561 atgaaaccta cgtagtgcta agagaagtgg cagttgccaa atacagcaac cttggtggtg
1621 gcatggacga gctgttcaag taatctagag tcgacccagg catcaaataa aacgaaaggc
1681 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
1741 taggacaaat gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
1801 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
1861 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgg gtgtggaaag
1921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
1981 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
2101 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagccacc
2161 atggtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
2221 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
2281 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
2341 atcctgtccc ctcagttcat gtacggctcc agggccttca tcaagcaccc cgccgacatc
2401 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
2461 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
2521 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
2581 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
2641 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
2701 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
2761 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
2821 cgccactcca ccggcggcat ggacgagctg tacaagtgac cggcaataaa aagacagaat
2881 aaaacgcacg gtgttgggtc gtttgttcgg atccgggctg gcgtaatagc gaagaggccc
2941 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag
3001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
3121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
3181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
3241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
3301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
3361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
3421 caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt
3481 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
3541 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
3601 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
3661 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
3721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
3781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
3841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
3901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
3961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
4021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
4081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
4141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
4201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
4261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
4321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
4381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
4441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
4501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
4561 tttattgctg ataaatctgg agccggtgag cgtggctctc gcggtatcat tgcagcactg
4621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
4681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
4741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
4801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
4861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
4921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
4981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5041 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
5101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5221 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5281 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5341 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5401 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
5461 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5521 ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat cttcaatatt
5581 ggccattagc catattattc attggttat
//
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