pEJS614_pTetR-P2A-BFPnls/sgNS

LOCUS        pEJS614_pTetR-P2A-BFPnls/sgNS                8612 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Non-specific Spy sgRNA under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pEJS614_pTetR-P2A-BFPnls/sgNS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Gao , Xin D., Li-Chun Tu, Aamir Mir, Tomas Rodriguez, Yuehe Ding, 
            John Leszyk, Job Dekker, Scott A. Shaffer, Lihua Julie Zhu, Scot A. 
  TITLE     C-BERST: defining subnuclear proteomic landscapes at genomic 
            elements with dCas9–APEX2
  JOURNAL   Nature Methods (2018)
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        125..155
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     222..242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        270..496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             497..677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    724..849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1342..1575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1760..1804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2102..2342
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     2102..2122
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2273..2292
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2506..2623
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2672..3182
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3101..3119
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3191..3763
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3771..3790
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3818..3836
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3847..4464
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESG"
     primer_bind     complement(3920..3939)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4501..4557
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4573..5283
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGIYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5284..5304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             5400..5633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5705..5826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5742..5761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5796..5815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5866..6001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5928..5947
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        complement(6022..6040)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6050..6067)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6050..6066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6059..6081)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6208..6663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6295..6314)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6505..6526
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6689..6793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6794..7654
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7012..7031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7825..8413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8314..8333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8567..8584
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      241 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
      301 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
      361 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
      421 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
      481 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      541 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      601 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      661 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      721 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
      781 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
      841 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
      901 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
      961 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1021 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1081 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1141 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1201 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1261 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1321 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1381 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1441 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1501 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1561 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1621 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1681 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1741 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     1801 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     1861 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     1921 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     1981 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2041 agtgaacgga tctcgacggt atcgatcacg agactagcct cgagcggccg cccccttcac
     2101 cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2161 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2221 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2281 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2341 acgaaacacc gaatctcgct tatataacga ggtttaagag ctatgctgga aacagcatag
     2401 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     2461 ttttgaattc tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2521 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2581 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2641 cagcagagat ccactttggc cgcggctcga gggggttggg gttgcgcctt ttccaaggca
     2701 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     2761 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2821 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2881 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     2941 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     3001 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     3061 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     3121 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     3181 agggggatcc gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
     3241 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
     3301 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
     3361 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
     3421 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
     3481 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     3541 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     3601 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     3661 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     3721 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3781 ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga
     3841 attgatatgt ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat
     3901 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag
     3961 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag
     4021 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt
     4081 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa
     4141 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc
     4201 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg
     4261 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg
     4321 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat
     4381 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa
     4441 aaacaactta aatgtgaaag tgggtccgcg tacagcggct cccgggagtt cgctagcggt
     4501 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctggt
     4561 agtggaacgc gtatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
     4621 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     4681 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     4741 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     4801 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     4861 gtcaccacat acgaagatgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     4921 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     4981 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     5041 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     5101 aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcatc
     5161 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     5221 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     5281 aatccaaaga aaaagcggaa agtgtgagtc gacctcgagg gggggcccgg tacctttaag
     5341 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5401 ggaagggcta attcactccc aacgaagaaa agatctgctt tttgcttgta ctgggtctct
     5461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5641 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5701 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5821 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     5881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6001 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     6061 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6121 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6181 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     6241 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6301 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6361 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6421 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6481 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6541 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6601 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     6661 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6781 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6841 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6901 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6961 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7021 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7081 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7141 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7201 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7261 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7321 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7381 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7441 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7501 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7561 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7621 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7681 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7741 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7801 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7861 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7921 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7981 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8041 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8101 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8161 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8221 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8281 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8341 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8401 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8461 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8521 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8581 cgaggaagcg gaagagcgcc caatacgcaa ac
//