pLemiR-NS

LOCUS       pLemiR-NS              11662 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pLemiR-NS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 11662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        2753..3056
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3057..3260
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3211..3231
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3257..3281
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3364..4059
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           4069..4190
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4218..4232
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4260..4386
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     misc_feature    4440..5012
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4607..4624)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4834..4853
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5015..5617
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5514..5534
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5633..6221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5686..5706)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6104..6115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6428..6661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6762..6779)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6768..6992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7038..7466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7125..7144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7335..7356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7475..7495)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7480..7809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7660..7795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7722..7741
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9017..9138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9187..9203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9187..9203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9200..9222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9211..9227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9235..9265)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9280..9301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9418..9435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9589..10177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9669..9688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10348..11208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10971..10990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11209..11313)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11361..11495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gagaccggtg gttaacgcgg atcccatcga ttgggagttc cgcgttacat
     2761 aacttaccgt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2821 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2881 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     2941 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     3001 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     3061 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     3121 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     3181 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     3241 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     3301 gctgttttga cctccataga agacaccgac tctagctaga ggctagcgct accggtcgcc
     3361 accatgagcg agctgatcaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     3421 aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     3481 accatgaaga tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     3541 accagcttca tgtacggcag caaagccttc atcaaccaca cccagggcat ccccgacttc
     3601 tttaagcagt ccttccctga gggcttcaca tgggagagaa tcaccacata cgaagacggg
     3661 ggcgtgctga ccgctaccca ggacaccagc ttccagaacg gctgcatcat ctacaacgtc
     3721 aagatcaacg gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacacgcggc
     3781 tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctgagagg ccacagccag
     3841 atggccctga agctcgtggg cgggggctac ctgcactgct ccttcaagac cacatacaga
     3901 tccaagaaac ccgctaagaa cctcaagatg cccggcttcc acttcgtgga ccacagactg
     3961 gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgagat ggctgtggcc
     4021 aagtactgcg acctccctag caaactgggg cacagataac tcgactgttt gaatgaggct
     4081 tcagtacttt acagaatcgt tgcctgcaca tcttggaaac acttgctggg attacttctt
     4141 caggttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga gcgatctcgc
     4201 ttgggcgaga gtaagtagtg aagccacaga tgtacttact ctcgcccaag cgagagtgcc
     4261 tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
     4321 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat
     4381 cacttttttc aattggaaga ctaatgcggc cggccacgcg tcggcggccg caaattccgc
     4441 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4501 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4561 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4621 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4681 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4741 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4801 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4861 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     4921 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4981 gacgtggttt tcctttgaaa aacacgataa taccatggcc accgagtaca agcccacggt
     5041 gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc
     5101 cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga
     5161 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     5221 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     5281 cgccgagatc ggctcgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     5341 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5401 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5461 ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5521 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5581 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtt cgcgtctgga acaatcaacc
     5641 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5701 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5761 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5821 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5881 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5941 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6001 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     6061 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     6121 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     6181 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt
     6241 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     6301 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     6361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6421 aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6661 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6721 gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg tgccttctag
     6781 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6841 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6901 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6961 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7021 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7081 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7141 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7201 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7261 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7321 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7381 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7441 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7501 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7561 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7621 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7681 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7741 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7801 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
     7861 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     7921 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     7981 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     8041 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     8101 gaattaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     8161 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     8221 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     8281 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     8341 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     8401 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     8461 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     8521 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     8581 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     8641 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     8701 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     8761 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     8821 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     8881 ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     8941 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9001 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9121 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     9181 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9241 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9301 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9361 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9421 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9481 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9541 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9601 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9661 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9721 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9781 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9841 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9901 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9961 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10021 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10081 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10141 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10201 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10261 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10321 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10381 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10441 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10501 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10561 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10621 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10681 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10741 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10801 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10861 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10921 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10981 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11041 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11101 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11161 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11221 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11281 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11341 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    11401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11461 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11521 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11581 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11641 tatgtactac aaacttagta gt
//