| 质粒名称: | pAAVLINK-EF1alpha-5'-PLXNA3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001877 |
| 产品用途: | 5' AAVLINK plasmid for PLXNA3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PLXNA3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-5'-PLXNA3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-5'-PLXNA3载体质粒基本信息
pAAVLINK-EF1alpha-5'-PLXNA3质粒图谱载体图谱和pAAVLINK-EF1alpha-5'-PLXNA3载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-5'-PLXNA3质粒载体简介
pAAVLINK-EF1alpha-5'-PLXNA3质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-5'-PLXNA3 7013 bp DNA circular SYN 25-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7013)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7013
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..4153
/codon_start=1
/gene="PLXNA3"
/gene_synonym="6.3; HSSEXGENE; PLXN3; PLXN4; XAP-6"
/product="plexin-A3 precursor"
/label=PLXNA3
/note="Sex chromosome X transmembrane protein of
HGFreceptor family 3; plexin-4; semaphorin receptor SEX"
/db_xref="GI:533112479"
/db_xref="CCDS:CCDS14752.1"
/db_xref="GeneID:55558"
/db_xref="HGNC:HGNC:9101"
/db_xref="MIM:300022"
/protein_id="NP_059984.3"
/translation="MPSVCLLLLLFLAVGGALGNRPFRAFVVTDTTLTHLAVHRVTGEV
FVGAVNRVFKLAPNLTELRAHVTGPVEDNARCYPPPSMRVCAHRLAPVDNINKLLLIDY
AARRLVACGSIWQGICQFLRLDDLFKLGEPHHRKEHYLSGAQEPDSMAGVIVEQGQGPS
KLFVGTAVDGKSEYFPTLSSRKLISDEDSADMFSLVYQDEFVSSQIKIPSDTLSLYPAF
DIYYIYGFVSASFVYFLTLQLDTQQTLLDTAGEKFFTSKIVRMCAGDSEFYSYVEFPIG
CSWRGVEYRLVQSAHLAKPGLLLAQALGVPADEDVLFTIFSQGQKNRASPPRQTILCLF
TLSNINAHIRRRIQSCYRGEGTLALPWLLNKELPCINTPMQINGNFCGLVLNQPLGGLH
VIEGLPLLADSTDGMASVAAYTYRQHSVVFIGTRSGSLKKVRVDGFQDAHLYETVPVVD
GSPILRDLLFSPDHRHIYLLSEKQVSQLPVETCEQYQSCAACLGSGDPHCGWCVLRHRC
CREGACLGASAPHGFAEELSKCVQVRVRPNNVSVTSPGVQLTVTLHNVPDLSAGVSCAF
EAAAENEAVLLPSGELLCPSPSLQELRALTRGHGATRTVRLQLLSKETGVRFAGADFVF
YNCSVLQSCMSCVGSPYPCHWCKYRHTCTSRPHECSFQEGRVHSPEGCPEILPSGDLLI
PVGVMQPLTLRAKNLPQPQSGQKNYECVVRVQGRQQRVPAVRFNSSSVQCQNASYSYEG
DEHGDTELDFSVVWDGDFPIDKPPSFRALLYKCWAQRPSCGLCLKADPRFNCGWCISEH
RCQLRTHCPAPKTNWMHLSQKGTRCSHPRITQIHPLVGPKEGGTRVTIVGDNLGLLSRE
VGLRVAGVRCNSIPAEYISAERIVCEMEESLVPSPPPGPVELCVGDCSADFRTQSEQVY
SFVTPTFDQVSPSRGPASGGTRLTISGSSLDAGSRVTVTVRDSECQFVRRDAKAIVCIS
PLSTLGPSQAPITLAIDRANISSPGLIYTYTQDPTVTRLEPTWSIINGSTAITVSGTHL
LTVQEPRVRAKYRGIETTNTCQVINDTAMLCKAPGIFLGRPQPRAQGEHPDEFGFLLDH
VQTARSLNRSSFTYYPDPSFEPLGPSGVLDVKPGSHVVLK"
misc_feature 727..732
/label=SDS
misc_feature 730..735
/label=SDS
misc_feature 1410..1415
/label=SDS
misc_feature 2730..2735
/label=SDS
misc_feature 2785..2790
/label=SDS
misc_feature 3055..3060
/label=SDS
misc_feature 3271..3276
/label=SDS
misc_feature 4062..4067
/label=SDS
intron 4154..4194
/label=Splicing Donor
misc_feature 4198..4231
/label=lox2272-JT1571
repeat_region 4276..4416
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4276..4405
/label=AAV2 ITR
repeat_region 4277..4417
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4491..4946
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5228..5332
/gene="bla"
/label=AmpR promoter
CDS 5333..6193
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6364..6952
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgccctc
721 tgtctgcctc ctcctgctgc tcttccttgc cgtggggggg gccctgggca acaggccctt
781 ccgtgccttc gtggtgacag acaccacgct tacccacctg gctgtgcacc gggtgactgg
841 ggaggtgttc gtgggcgcag tgaaccgagt ctttaagctg gcccccaacc tgactgagct
901 gcgggcccat gtcacggggc ccgtcgagga caacgctcgc tgctacccgc cccccagcat
961 gcgcgtgtgt gcccaccgcc tggcccccgt ggacaacatc aacaagctgc tgctcataga
1021 ctatgcggcc cgccgcctgg tggcctgcgg cagcatctgg cagggcatct gccagttcct
1081 gcgtctggac gacctcttca agctgggtga gccgcaccac cgcaaggagc actacctgtc
1141 gggggcccag gagcccgact ccatggctgg tgtcattgtg gagcagggcc aggggcccag
1201 caagctgttt gtgggcactg ctgtcgacgg caagtcggag tacttcccca ccttgagctc
1261 ccgcaagctc atcagtgatg aagacagcgc ggacatgttc agtctcgtgt accaggatga
1321 gtttgtgtcc tcccagatca agatcccctc agacacgctg tccttgtacc ctgcctttga
1381 catctactac atctacggct tcgtcagcgc ctccttcgtg tacttcctga cgctgcagct
1441 ggacacccag cagacgctgt tggacacagc gggcgagaaa tttttcacgt ccaagatcgt
1501 gcgcatgtgc gcgggagact cagagttcta ctcatacgtg gaattcccca tcggctgctc
1561 ctggcgcggc gtggagtacc gcttggtgca gagcgcccac ctggccaagc ctggcctgct
1621 gctggcccag gccctgggcg tgccggctga tgaggacgtc ctcttcacca tcttctctca
1681 gggccagaag aaccgggcca gcccaccccg gcagaccatc ctctgcctct tcaccctcag
1741 caacatcaat gcccacatcc ggcgccgcat ccagtcctgc tatcgtgggg agggcactct
1801 ggctctgccc tggctgctga acaaggagct gccctgcatc aacaccccca tgcagatcaa
1861 cggcaacttc tgtgggctgg tgttgaacca gcctctggga ggcctgcatg tgatcgaggg
1921 gctccccctg ctggccgaca gcaccgacgg catggccagc gtggccgcct acacctaccg
1981 ccagcactct gtggtcttca ttggcacgcg cagcggcagc ttgaagaagg tgcgggtcga
2041 tggcttccag gatgcccacc tgtatgagac agtccccgtg gtggatggca gccccatcct
2101 ccgagacctg ctcttcagcc cggaccaccg gcacatctat ctcctgagtg agaagcaggt
2161 gagccagctc ccggtggaga cctgtgagca gtaccagagc tgcgcagcct gcctgggctc
2221 cggggacccg cactgtggtt ggtgtgtgct gcgacacagg tgctgccgcg aaggggcctg
2281 tctgggcgcc tctgccccac acggctttgc tgaggagctg agcaagtgtg tccaggtgcg
2341 ggtccggccc aacaatgtgt cagtgacgtc acctggggtg cagctgaccg tcaccctgca
2401 caacgtgcca gacctcagtg cgggcgtgag ctgcgccttc gaggcggcgg cggagaacga
2461 ggcggtcctg ctgccctccg gtgaactgct ctgcccctca ccctccctcc aggagctccg
2521 agctcttacc agggggcatg gggccacccg cactgtgcgg ctgcagcttc tctccaagga
2581 gacaggcgtg aggtttgccg gtgctgactt tgtcttctac aactgcagcg tcctccagtc
2641 gtgcatgtcc tgtgttggca gcccttaccc ctgccactgg tgtaagtacc gccacacgtg
2701 taccagccgc ccccacgagt gctccttcca ggagggcagg gtccacagcc ctgagggctg
2761 ccctgagatc ctgcccagtg gggacctcct gatccccgtt ggggtcatgc agcctcttac
2821 cttgcgggct aagaacctac ctcagccgca gtcgggccag aagaactatg agtgcgtggt
2881 gcgggtgcag gggcggcagc agcgggtgcc tgccgtgcgc ttcaacagca gcagtgtgca
2941 gtgccagaac gcctcgtact cctatgaagg tgatgagcat ggtgacaccg agctggactt
3001 ctccgtggtc tgggatggag acttccccat agacaagcct cccagcttcc gagccctcct
3061 gtacaagtgc tgggcgcagc ggcccagctg tggcctctgc ctcaaggctg atccccgctt
3121 caactgtggc tggtgcatct cagagcacag gtgccagctg cggacccact gcccggcccc
3181 gaagaccaac tggatgcacc tgagccagaa gggcacccgg tgcagccacc cccgcatcac
3241 gcagatccac cctctcgtgg ggcccaagga aggaggcacc cgggtcacca tcgtgggtga
3301 caacctgggc ctcttgtccc gagaggtggg cctgcgggtg gctggcgtgc gttgcaactc
3361 cattccggcc gagtacatca gtgctgagag gatcgtgtgt gagatggagg agtcgctggt
3421 gcccagcccg ccgccggggc ccgtggagct gtgtgtgggt gactgttcag ccgacttccg
3481 cacgcagtcg gagcaggtct acagctttgt gaccccaacg tttgaccaag tgagtcccag
3541 ccgtggcccg gcgtccgggg gcacacggct taccatctca ggcagctctc tggatgctgg
3601 cagcagggtc acagtgactg tgagggacag cgagtgccag tttgtaagga gagatgccaa
3661 ggcgatcgtg tgcatctcac ctctctccac cctgggcccc agccaggccc ccatcacact
3721 tgccattgac cgggctaaca tctccagccc cgggctcatc tacacctaca ctcaggaccc
3781 caccgtcacc cgccttgagc ccacctggag catcatcaat ggaagcactg ccatcactgt
3841 gagtgggacc cacctgctga cggtccagga gccccgggtc cgtgccaagt accgcggcat
3901 tgagaccacc aatacatgcc aagtgatcaa cgacactgcc atgctgtgta aggcccccgg
3961 catctttctt gggcggcccc agcctcgggc gcaaggcgag caccctgatg agtttggctt
4021 cctgctggac cacgtgcaaa cggcccgctc cctcaaccgc tcctccttta cctactaccc
4081 tgatcccagc tttgagccgc tggggccctc tggcgtgctg gacgtcaaac cgggctccca
4141 cgtggtgctg aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
4201 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
4261 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4321 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4381 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4441 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4561 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4681 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4801 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4861 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4921 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4981 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
5041 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
5101 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
5161 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
5221 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
5401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5881 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
6001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
6061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
6181 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
6241 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
6301 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
6361 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6421 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6481 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6541 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6601 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6661 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6721 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6781 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6841 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6901 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6961 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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