| 质粒名称: | pQL52 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002501 |
| 产品用途: | Synthetic mammalian ETS TF-dependent SEAP expression vector ((GGAA)14-PhCMVmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
Synthetic
|
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
pQL52
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL52载体质粒基本信息
pQL52质粒图谱载体图谱和pQL52载体序列质粒序列多克隆位点信息
pQL52质粒载体简介
pQL52质粒序列载体序列
LOCUS pQL52 6466 bp DNA circular SYN 19-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 6466)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6466
/mol_type="other DNA"
/organism="recombinant plasmid"
primer_bind 218..237
/label=QL143-F
primer_bind 222..269
/label=QL101 GA-F
misc_feature 238..265
/label=GGAA
primer_bind complement(242..299)
/label=QL 102 GA-R
primer_bind complement(254..294)
/label=QL 102 GA-R
misc_feature 266..293
/label=GGAA
misc_feature 300..447
/label=hcmvmin
misc_feature 369..383
/label=New Feature
primer_bind complement(432..447)
/label=oYW11
misc_feature 470..493
/label=New Feature
primer_bind 494..520
/label=Oscl13-1
misc_feature 495..2015
/label=SEAP
primer_bind complement(1995..2023)
/label=Oscl23-2
primer_bind complement(1997..2015)
/label=Oscl13-2
terminator 2063..2290
/label=bGH PA
primer_bind complement(2273..2293)
/label=QL153-R
misc_feature 2958..3035
/label=SV40 ORI
CDS 3177..3968
/label=Kan/neoR
primer_bind complement(4308..4328)
/label=M13-rev
misc_binding complement(4334..4356)
/label=LacO
promoter complement(4361..4390)
/label=lac
CDS complement(5473..6132)
/label=AmpR
promoter complement(6372..6400)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacgga
241 aggaaggaag gaaggaagga aggaaggaag gaaggaagga aggaaggaag gaagctagct
301 cgagctcggt acccgggtcg agtaggcgtg tacggtggga ggcctatata agcagagctc
361 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
421 gacaccggga ccgatccagc ctccgcggcc ccgaattcga gctcgcccgg ggatcctcta
481 gagtcagctt ctgcatgctg ctgctgctgc tgctgctggg cctgaggcta cagctctccc
541 tgggcatcat cccagttgag gaggagaacc cggacttctg gaaccgcgag gcagccgagg
601 ccctgggtgc cgccaagaag ctgcagcctg cacagacagc cgccaagaac ctcatcatct
661 tcctgggcga tgggatgggg gtgtctacgg tgacagctgc caggatccta aaagggcaga
721 agaaggacaa actggggcct gagatacccc tggccatgga ccgcttccca tatgtggctc
781 tgtccaagac atacaatgta gacaaacatg tgccagacag tggagccaca gccacggcct
841 acctgtgcgg ggtcaagggc aacttccaga ccattggctt gagtgcagcc gcccgcttta
901 accagtgcaa cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag
961 cagggaagtc agtgggagtg gtaaccacca cacgagtgca gcacgcctcg ccagccggca
1021 cctacgccca cacggtgaac cgcaactggt actcggacgc cgacgtgcct gcctcggccc
1081 gccaggaggg gtgccaggac atcgctacgc agctcatctc caacatggac attgacgtga
1141 tcctaggtgg aggccgaaag tacatgtttc ccatgggaac cccagaccct gagtacccag
1201 atgactacag ccaaggtggg accaggctgg acgggaagaa tctggtgcag gaatggctgg
1261 cgaagcgcca gggtgcccgg tatgtgtgga accgcactga gctcatgcag gcttccctgg
1321 acccgtctgt gacccatctc atgggtctct ttgagcctgg agacatgaaa tacgagatcc
1381 accgagactc cacactggac ccctccctga tggagatgac agaggctgcc ctgcgcctgc
1441 tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc
1501 atcatgaaag cagggcttac cgggcactga ctgagacgat catgttcgac gacgccattg
1561 agagggcggg ccagctcacc agcgaggagg acacgctgag cctcgtcact gccgaccact
1621 cccacgtctt ctccttcgga ggctaccccc tgcgagggag ctccatcttc gggctggccc
1681 ctggcaaggc ccgggacagg aaggcctaca cggtcctcct atacggaaac ggtccaggct
1741 atgtgctcaa ggacggcgcc cggccggatg ttaccgagag cgagagcggg agccccgagt
1801 atcggcagca gtcagcagtg cccctggacg aagagaccca cgcaggcgag gacgtggcgg
1861 tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag accttcatag
1921 cgcacgtcat ggccttcgcc gcctgcctgg agccctacac cgcctgcgac ctggcgcccc
1981 ccgccggcac caccgacgcc gcgcacccgg gttaagcggc cgctcgagtc tagagggccc
2041 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
2101 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
2161 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
2221 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
2281 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
2341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
2401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
2461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
2521 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
2581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
2641 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
2701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
2761 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
2821 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
2881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
2941 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
3001 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
3061 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
3121 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
3181 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
3241 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
3301 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
3361 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
3421 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
3481 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
3541 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
3601 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
3661 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
3721 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
3781 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
3841 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
3901 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
3961 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
4021 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
4081 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
4141 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
4201 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
4261 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
4321 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
4381 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
4441 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
4501 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
4561 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4621 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4681 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4741 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4801 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4861 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4921 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4981 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
5041 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
5101 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
5161 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
5221 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
5281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
5341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
5401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
5461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
5521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
5581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
5641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
5701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
5761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
5821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
5881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
5941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
6001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
6061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
6121 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
6181 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
6241 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
6301 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
6361 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
6421 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
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