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pDsRed-Monomer-C1载体质粒基本信息
pDsRed-Monomer-C1质粒图谱载体图谱和pDsRed-Monomer-C1载体序列质粒序列多克隆位点信息
pDsRed-Monomer-C1质粒载体简介
pDsRed-Monomer-C1质粒序列载体序列
LOCUS pDsRed-Monomer-C1 4689 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Vector for fusing DsRed-Monomer to the N-terminus of a partner
protein.
ACCESSION .
VERSION .
KEYWORDS pDsRed-Monomer-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4689)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Thursday, February 20, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4689
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1287
/codon_start=1
/product="monomeric derivative of DsRed fluorescent
protein"
/note="DsRed-Monomer"
/note="mammalian codon-optimized"
/translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
GSQ"
misc_feature 1288..1353
/note="MCS"
/note="multiple cloning site of fluorescent protein
plasmids"
misc_feature 1362..1372
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 1477..1598
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1605..2060)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2087..2191
/gene="bla"
/note="AmpR promoter"
promoter 2193..2550
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2401..2536
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2585..3379
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 3611..3658
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 3987..4575
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggacaa caccgaggac gtcatcaagg agttcatgca gttcaaggtg
661 cgcatggagg gctccgtgaa cggccactac ttcgagatcg agggcgaggg cgagggcaag
721 ccctacgagg gcacccagac cgccaagctg caggtgacca agggcggccc cctgcccttc
781 gcctgggaca tcctgtcccc ccagttccag tacggctcca aggcctacgt gaagcacccc
841 gccgacatcc ccgactacat gaagctgtcc ttccccgagg gcttcacctg ggagcgctcc
901 atgaacttcg aggacggcgg cgtggtggag gtgcagcagg actcctccct gcaggacggc
961 accttcatct acaaggtgaa gttcaagggc gtgaacttcc ccgccgacgg ccccgtaatg
1021 cagaagaaga ctgccggctg ggagccctcc accgagaagc tgtaccccca ggacggcgtg
1081 ctgaagggcg agatctccca cgccctgaag ctgaaggacg gcggccacta cacctgcgac
1141 ttcaagaccg tgtacaaggc caagaagccc gtgcagctgc ccggcaacca ctacgtggac
1201 tccaagctgg acatcaccaa ccacaacgag gactacaccg tggtggagca gtacgagcac
1261 gccgaggccc gccactccgg ctcccagtcc ggactcagat ctcgagctca agcttcgaat
1321 tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
1381 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
1441 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
1501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
1561 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
1621 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
1681 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
1741 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
1801 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
1861 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
1921 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
1981 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
2041 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
2101 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
2161 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
2221 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2281 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
2341 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
2401 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
2461 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
2521 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
2581 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
2641 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
2701 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
2761 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
2821 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
2881 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
2941 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
3001 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
3061 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
3121 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
3181 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
3241 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
3301 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
3361 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
3421 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
3481 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
3541 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
3601 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
3661 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
3721 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
3781 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
3841 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
3901 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
3961 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
4021 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
4081 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
4141 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
4201 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
4261 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
4321 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
4381 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
4441 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
4501 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
4561 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
4621 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
4681 gccatgcat
//
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