pRGE31

LOCUS       pRGE31                9188 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Expressing sgRNA and Cas9 in plants. The sgRNA was controlled by 
            rice snoRNA U3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pRGE31
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      71..323
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(332..349)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(332..348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..363)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      561..1016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(648..667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     858..879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1033..1052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1152..1174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1212..1230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1298..1402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1403..2263
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGTRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1621..1640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2923..2942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3176..3193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3310..3331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3346..3376
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3384..3400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3389..3411
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3408..3424
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3408..3424
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3443..3823
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        3840..3915
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4481..4826
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4769..4789
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4848..4872
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4881..4946
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4953..4973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4998..9098
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9099..9146
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    complement(9179..15)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa cgtcgtgact
      361 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      421 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      481 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      541 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      601 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      661 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      721 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
      781 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      841 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      901 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      961 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     1021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2101 tggagccggt gagcgtggca ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3181 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3241 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3301 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3361 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3421 tgaccatgat tacgccaagc ttaaggaatc tttaaacata cgaacagatc acttaaagtt
     3481 cttctgaagc aacttaaagt tatcaggcat gcatggatct tggaggaatc agatgtgcag
     3541 tcagggacca tagcacaaga caggcgtctt ctactggtgc taccagcaaa tgctggaagc
     3601 cgggaacact gggtacgttg gaaaccacgt gatgtgaaga agtaagataa actgtaggag
     3661 aaaagcattt cgtagtgggc catgaagcct ttcaggacat gtattgcagt atgggccggc
     3721 ccattacgca attggacgac aacaaagact agtattagta ccacctcggc tatccacata
     3781 gatcaaagct gatttaaaag agttgtgcag atgatccgtg gcaggagacc gaggtctcgg
     3841 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     3901 gcaccgagtc ggtgcttttt tgttttagag ctagaaatag caagttaaaa taaggctagt
     3961 ccgtttttag cgcgtgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga
     4021 atgctaaccc acagatggtt agagaggctt acgcagcagc actcatcaag acgatctacc
     4081 cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa
     4141 gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta
     4201 ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg
     4261 gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag
     4321 aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac
     4381 tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca
     4441 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
     4501 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
     4561 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
     4621 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     4681 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     4741 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     4801 gaagttcatt tcatttggag agaacacggg ggactctaga gttatcaaca agtttgtaca
     4861 aaaaagcagg ctccaccatg gactataagg accacgacgg agactacaag gatcatgata
     4921 ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc
     4981 acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg
     5041 tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg
     5101 gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg
     5161 gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga
     5221 agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca
     5281 gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc
     5341 accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct
     5401 accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc
     5461 tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
     5521 ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt
     5581 tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac
     5641 tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg
     5701 gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact
     5761 tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg
     5821 acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc
     5881 tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc
     5941 ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga
     6001 aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca
     6061 agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca
     6121 tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag
     6181 aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc
     6241 tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca
     6301 accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg
     6361 ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct
     6421 ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga
     6481 ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg
     6541 agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa
     6601 agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca
     6661 accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg
     6721 actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg
     6781 atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc
     6841 tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc
     6901 tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat
     6961 acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg
     7021 gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc
     7081 tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc
     7141 agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg
     7201 gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc
     7261 ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga
     7321 acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc
     7381 tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc
     7441 tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact
     7501 acgatgtgga ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg
     7561 tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg
     7621 tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa
     7681 agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct
     7741 tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg
     7801 actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga
     7861 tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc
     7921 gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg
     7981 ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt
     8041 acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt
     8101 acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg
     8161 agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata
     8221 agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga
     8281 aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca
     8341 gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca
     8401 gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga
     8461 aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga
     8521 agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca
     8581 tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct
     8641 ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc
     8701 tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac
     8761 agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt
     8821 tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca
     8881 agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga
     8941 ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt
     9001 acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt
     9061 acgagacacg gatcgacctg tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa
     9121 aggccggcca ggcaaaaaag aaaaagtaag aattcgcggc cgcactcgag atatctagac
     9181 ccagcttt
//