| 质粒名称: | pAAV-CAG-E2-Crimson |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000047 |
| 产品用途: | AAV, Expresses E2-Crimson in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
E2-Crimson
|
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-E2-Crimson
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-E2-Crimson载体质粒基本信息
pAAV-CAG-E2-Crimson质粒图谱载体图谱和pAAV-CAG-E2-Crimson载体序列质粒序列多克隆位点信息
pAAV-CAG-E2-Crimson质粒载体简介
pAAV-CAG-E2-Crimson质粒序列载体序列
LOCUS pAAV-CAG-E2-Crimson 5401 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5401)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5401
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 954..1007
/label=0515-PM662-F
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1657
/codon_start=1
/product="far-red noncytotoxic tetrameric variant of DsRed
fluorescent protein (Strack et al., 2009)"
/label=E2-Crimson
/note="mammalian codon-optimized"
/translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
LFQ"
primer_bind complement(1638..1686)
/label=0503-E2-Crimson-R
misc_feature 1685..2273
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1752..1777)
/label=WPRE-R
CDS complement(2156..2167)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2316..2437
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2965..3553)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3724..4584)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4585..4689)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4715..5170)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggatag cactgagaac gtcatcaagc ccttcatgcg
1021 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgtggg
1081 cgagggcaag ccctacgagg gcacccagac cgccaagctg caagtgacca agggcggccc
1141 cctgcccttc gcctgggaca tcctgtcccc ccagttcttc tacggctcca aggcgtacat
1201 caagcacccc gccgacatcc ccgactacct caagcagtcc ttccccgagg gcttcaagtg
1261 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
1321 gcaggacggc accctcatct accacgtgaa gttcatcggc gtgaacttcc cctccgacgg
1381 ccccgtaatg cagaagaaga ctctgggctg ggagccctcc actgagcgca actacccccg
1441 cgacggcgtg ctgaagggcg agaaccacat ggcgctgaag ctgaagggcg gcggccacta
1501 cctgtgtgag ttcaagtcca tctacatggc caagaagccc gtgaagctgc ccggctacca
1561 ctacgtggac tacaagctcg acatcacctc ccacaacgag gactacaccg tggtggagca
1621 gtacgagcgc gccgaggccc gccaccacct gttccagtaa gaattcgata tcaagcttat
1681 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
1741 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
1801 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
1861 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
1921 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
1981 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
2041 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
2101 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
2161 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
2221 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
2281 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
2341 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
2401 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
2461 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
2521 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
2581 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
2641 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
2701 cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag
2761 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
2821 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
2881 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
2941 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
3001 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
3061 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
3121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
3181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
3241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
3301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
3361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
3421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
3481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
3541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
3601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
3661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
3721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
3781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
3841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
3901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
3961 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
4021 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
4081 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
4141 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
4201 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
4261 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
4321 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
4381 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
4441 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
4501 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
4561 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
4621 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
4681 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
4741 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
4801 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
4861 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
4921 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
4981 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
5041 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
5101 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
5161 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
5221 gcctcttcgc tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
5281 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
5341 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
5401 t
//
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