pAAV-CAG-RGEPO2

LOCUS       pAAV-CAG-RGEPO2           5764 bp DNA     circular SYN 22-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(160..748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(919..1779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1780..1884)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1910..2365)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2904..3181
                     /label=chicken beta-actin promoter
     primer_bind     3582..3623
                     /label=Intrac-cag-F1
     primer_bind     3605..3628
                     /label=N-mapple-F2
     primer_bind     3605..3623
                     /label=Intra-F1
     misc_feature    3605..3610
                     /label=kozak
     CDS             3611..4063
                     /codon_start=1
                     /label=N-mApple
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
                     EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
     primer_bind     4037..4084
                     /label=FR-1-3-F
     primer_bind     4037..4058
                     /label=FR-Y-Ra-F
     primer_bind     complement(4050..4063)
                     /label=N-mapple-R
     primer_bind     complement(4052..4070)
                     /label=FR-1-3-R
     primer_bind     complement(4059..4077)
                     /label=0905-R1
     primer_bind     4060..4099
                     /label=PA-S9A-F1
     primer_bind     4061..4093
                     /label=PA-F6A-F1
     primer_bind     4061..4084
                     /label=PA-K8A-F1
     CDS             4064..4066
                     /codon_start=1
                     /label=LINKER1
                     /translation="L"
     CDS             4067..4498
                     /codon_start=1
                     /label=YgaU_Hydrothermal
                     /translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
                     VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
                     ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
     primer_bind     4100..4117
                     /label=0811-F2
     primer_bind     4257..4289
                     /label=PA-T73A-F1
     primer_bind     4261..4295
                     /label=PA-G75A-F1
     primer_bind     complement(4261..4278)
                     /label=0905-R2
     primer_bind     4298..4316
                     /label=0811-F1
     CDS             4499..4507
                     /codon_start=1
                     /label=LINKER
                     /translation="SNP"
     CDS             4508..4765
                     /codon_start=1
                     /label=C-mApple
                     /translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
                     GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
     primer_bind     complement(4746..4765)
                     /label=0821_FRCaMPi_R
     primer_bind     complement(4747..4765)
                     /label=PD-R1
     primer_bind     complement(4749..4790)
                     /label=Intra-cag-R1
     primer_bind     complement(4749..4768)
                     /label=c-mapple-PCI-R
     primer_bind     4751..4765
                     /label=0821_KA_FRCaMPi_F
     misc_feature    4793..5381
                     /label=WPRE
                     /note="woodchuck posttranscriptional regulatory element"
     CDS             complement(5264..5275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5424..5545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
       61 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      121 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      181 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      241 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      301 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      361 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      421 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      481 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      541 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      601 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      661 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      721 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      781 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      841 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      901 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      961 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1021 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1081 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1141 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1201 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1261 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1321 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1381 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1441 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1501 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1561 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1621 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1681 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1741 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1801 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1861 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     1921 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1981 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     2041 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     2101 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     2161 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     2221 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     2281 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     2341 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     2401 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgc gcgctcgctc gctcactgag
     2461 gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc agtgagcgag
     2521 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt
     2581 aacccgccat gctacttatc tacgtagcca tgctctagga agatcgtagg ttaattaagg
     2641 cgcgccgcct aggccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2701 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2761 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2821 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2881 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     2941 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     3001 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     3061 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     3121 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     3181 gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     3241 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     3301 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     3361 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg
     3421 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     3481 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     3541 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggggatccgg
     3601 taccgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     3661 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     3721 gggcgagggc cgcccctacg aggccttcca gaccgctagg ctgaaggtga ccaagggtgg
     3781 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     3841 cattaagcac ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag
     3901 gtgggagcgc gtgatgagct tcgaggacgg cggcattatt cacgttaatc aggactcctc
     3961 cctgcaggac ggcgtattca tctacaaggt gaagctgctc ggcaccaact tcccccccga
     4021 cggccccgta atgcagaaga agaccatggg ctgggaggct tccctgggca tctgggactt
     4081 cgtgaagagc gccggcagga agatcggcat cggcagcgag gaggccgccc ccgccgccga
     4141 cgacctgaag aaggagctgg acagctacaa gctgggcacc gacgacgtga aggtggaggt
     4201 ggtgggcgac aaggccgtga tcaccggcaa cgtggccgac cagagcatcc tggagaaggc
     4261 catcgtggcc gtgggcaaca ccctgggcat cagcaaggtg gagagcaacg tggaggtgcc
     4321 cgacgccaag gagcccgtgt tccacaccgt gaagaagggc gacaccctgt gggccatcgc
     4381 cagcaagcag tacggcaacg tcagcaagta caacgccatc ttcgaggcca acaagcccat
     4441 gctgagccac cccgacaaga tctaccccgg ccaggtgctg aggatccccg acctgggctc
     4501 taacccggtt tccgagtgga tgtaccccga ggacggcgcc ctgaagagcg agatcaggaa
     4561 ggggctgagg ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc
     4621 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcgagttgg acatcttgtc
     4681 ccacaacgag gactacacca tcgtggaaca gtgcgaacgc gccgtgggcc gccaccccac
     4741 cggtggcacg gacgcgctgt acaagtagga attcgatatc aagcttatcg ataatcaacc
     4801 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4861 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4921 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4981 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5041 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5161 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5221 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5281 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5341 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg
     5401 gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     5461 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     5521 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     5581 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     5641 cgataaggat cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta
     5701 actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     5761 ctga
//