pElectra2-Vimentin

LOCUS      pElectra2-Vimentin              6107 bp DNA     circular SYN 20-MAR-2025
DEFINITION  Vimentin fused to monomeric near-infrared fluorescent protein miRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum
            DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades
            JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C,
            Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, 
            Sabatini BL, Boyden ES
  TITLE     A robotic multidimensional directed evolution approach applied to 
            fluorescent voltage reporters.
  JOURNAL   Nat Chem Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9.
  PUBMED    29483642
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2018 Feb 26. pii: 10.1038/s41589-018-0004-9. doi: 
            10.1038/s41589-018-0004-9."
FEATURES             Location/Qualifiers
     source          1..6107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             612..1322
                     /codon_start=1
                     /label=Electra2
                     /translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTD
                     IALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAV
                     AKYSNLGGGMDELFK"
     primer_bind     1119..1139
                     /label=0623-CalypsoMid
     CDS             1368..2765
                     /codon_start=1
                     /label=Vimentin
                     /translation="STRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRP
                     STSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKV
                     ELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQL
                     TNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERK
                     VESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAK
                     NLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLE
                     RQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATY
                     RKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETS
                     QHHDDLE"
     polyA_signal    2895..3016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2932..2951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2986..3005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2986..3005)
                     /label=SV40-R-Universal
     rep_origin      complement(3023..3478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3160..3181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3372..3391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3505..3609
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3611..3968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3634..3654)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3819..3954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3881..3900
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3904..3915)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4003..4797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4057..4076)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4667..4686
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4985..5004)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5029..5076
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5405..5993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5894..5913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtgccac catggtgtct aagggcgaag agctgatcga ggaaaatatg cgtatgaagg
      661 tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca
      721 gaccgtacga gggagtgcaa accatgagga tcaaagtcat cgagggagga cccctgccat
      781 ttgcctttga cattcttgcc acgtcgttcc tgttcggcag caagaccttt atcaagtacc
      841 cggccgacat ccctgatttc ttcgaacagt cctttcctga gggctttact tgggaaagag
      901 ttacgagata cgaagatggt ggagtagtca ccgtcacgca ggacaccagc cttgaggatg
      961 gtgggctcgt ctacaacgtc aaggtcagag gggtaaactt tcactccaag ggtcccgtga
     1021 tgcagaagaa gaccgagggt tgggagcctt tcacagagat gatgtatcca gcagatggtg
     1081 gtctgagagg atataccgac atcgcactga aagttgatgg tggtggccat ctgcacgcca
     1141 acatcgtgac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc
     1201 atgccgttga ttaccgcctg gaaaggatcg aagagagtga caatgaaacc tacgtagtgc
     1261 taagagaagt ggcagttgcc aaatacagca accttggtgg tggcatggac gagctgttca
     1321 agagatctgg cagcggtgga ggcagcgcat ccggcggaag cggaagctcc accaggtccg
     1381 tgtcctcgtc ctcctaccgc aggatgttcg gcggcccggg caccgcgagc cggccgagct
     1441 ccagccggag ctacgtgact acgtccaccc gcacctacag cctgggcagc gcgctgcgcc
     1501 ccagcaccag ccgcagcctc tacgcctcgt ccccgggcgg cgtgtatgcc acgcgctcct
     1561 ctgccgtgcg cctgcggagc agcgtgcccg gggtgcggct cctgcaggac tcggtggact
     1621 tctcgctggc cgacgccatc aacaccgagt tcaagaacac ccgcaccaac gagaaggtgg
     1681 agctgcagga gctgaatgac cgcttcgcca actacatcga caaggtgcgc ttcctggagc
     1741 agcagaataa gatcctgctg gccgagctcg agcagctcaa gggccaaggc aagtcgcgcc
     1801 tgggggacct ctacgaggag gagatgcggg agctgcgccg gcaggtggac cagctaacca
     1861 acgacaaagc ccgcgtcgag gtggagcgcg acaacctggc cgaggacatc atgcgcctcc
     1921 gggagaaatt gcaggaggag atgcttcaga gagaggaagc cgaaaacacc ctgcaatctt
     1981 tcagacagga tgttgacaat gcgtctctgg cacgtcttga ccttgaacgc aaagtggaat
     2041 ctttgcaaga agagattgcc tttttgaaga aactccacga agaggaaatc caggagctgc
     2101 aggctcagat tcaggaacag catgtccaaa tcgatgtgga tgtttccaag cctgacctca
     2161 cggctgccct gcgtgacgta cgtcagcaat atgaaagtgt ggctgccaag aacctgcagg
     2221 aggcagaaga atggtacaaa tccaagtttg ctgacctctc tgaggctgcc aaccggaaca
     2281 atgacgccct gcgccaggca aagcaggagt ccactgagta ccggagacag gtgcagtccc
     2341 tcacctgtga agtggatgcc cttaaaggaa ccaatgagtc cctggaacgc cagatgcgtg
     2401 aaatggaaga gaactttgcc gttgaagctg ctaactacca agacactatt ggccgcctgc
     2461 aggatgagat tcagaatatg aaggaggaaa tggctcgtca ccttcgtgaa taccaagacc
     2521 tgctcaatgt taagatggcc cttgacattg agattgccac ctacaggaag ctgctggaag
     2581 gcgaggagag caggatttct ctgcctcttc caaacttttc ctccctgaac ctgagggaaa
     2641 ctaatctgga ttcactccct ctggttgata cccactcaaa aaggacactt ctgattaaga
     2701 cggttgaaac tagagatgga caggttatca acgaaacttc tcagcatcac gatgaccttg
     2761 aataaggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     2821 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     2881 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3001 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     3061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     3481 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3541 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3601 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     3661 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3721 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3781 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3841 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3901 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3961 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     4021 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     4081 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     4141 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     4201 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     4261 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     4321 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     4381 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     4441 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     4501 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     4561 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     4621 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     4681 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     4741 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     4801 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     4861 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     4921 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     4981 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     5041 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     5101 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     5161 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     5221 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     5281 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5341 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5401 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5461 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5521 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5581 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5641 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5701 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5761 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5821 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5881 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5941 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6001 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6061 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//