| 质粒名称: | pAAVLINK-EF1a-HA-5'-ABCA7 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001709 |
| 产品用途: | 5' AAVLINK plasmid for ABCA7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ABCA7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-ABCA7
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-ABCA7载体质粒基本信息
pAAVLINK-EF1a-HA-5'-ABCA7质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-ABCA7载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-ABCA7质粒载体简介
pAAVLINK-EF1a-HA-5'-ABCA7质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-ABCA7 6335 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6335)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6335
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3475
/codon_start=1
/label=ABCA7
/translation="MAFWTQLMLLLWKNFMYRRRQPVQLLVELLWPLFLFFILVAVRHS
HPPLEHHECHFPNKPLPSAGTVPWLQGLICNVNNTCFPQLTPGEEPGRLSNFNDSLVSR
LLADARTVLGGASAHRTLAGLGKLIATLRAARSTAQPQPTKQSPLEPPMLDVAELLTSL
LRTESLGLALGQAQEPLHSLLEAAEDLAQELLALRSLVELRALLQRPRGTSGPLELLSE
ALCSVRGPSSTVGPSLNWYEASDLMELVGQEPESALPDSSLSPACSELIGALDSHPLSR
LLWRRLKPLILGKLLFAPDTPFTRKLMAQVNRTFEELTLLRDVREVWEMLGPRIFTFMN
DSSNVAMLQRLLQMQDEGRRQPRPGGRDHMEALRSFLDPGSGGYSWQDAHADVGHLVGT
LGRVTECLSLDKLEAAPSEAALVSRALQLLAEHRFWAGVVFLGPEDSSDPTEHPTPDLG
PGHVRIKIRMDIDVVTRTNKIRDRFWDPGPAADPLTDLRYVWGGFVYLQDLVERAAVRV
LSGANPRAGLYLQQMPYPCYVDDVFLRVLSRSLPLFLTLAWIYSVTLTVKAVVREKETR
LRDTMRAMGLSRAVLWLGWFLSCLGPFLLSAALLVLVLKLGDILPYSHPGVVFLFLAAF
AVATVTQSFLLSAFFSRANLAAACGGLAYFSLYLPYVLCVAWRDRLPAGGRVAASLLSP
VAFGFGCESLALLEEQGEGAQWHNVGTRPTADVFSLAQVSGLLLLDAALYGLATWYLEA
VCPGQYGIPEPWNFPFRRSYWCGPRPPKSPAPCPTPLDPKVLVEEAPPGLSPGVSVRSL
EKRFPGSPQPALRGLSLDFYQGHITAFLGHNGAGKTTTLSILSGLFPPSGGSAFILGHD
VRSSMAAIRPHLGVCPQYNVLFDMLTVDEHVWFYGRLK"
intron 3476..3516
/label=Splicing Donor
misc_feature 3520..3553
/label=lox2272-JT1571
repeat_region 3598..3738
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3598..3727
/label=AAV2 ITR
rep_origin 3813..4268
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4550..4654
/gene="bla"
/label=AmpR promoter
CDS 4655..5515
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5686..6274
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccttctgg acacagctga tgctgctgct
781 ctggaagaat ttcatgtatc gccggagaca gccggtccag ctcctggtcg aattgctgtg
841 gcctctcttc ctcttcttca tcctggtggc tgttcgccac tcccacccgc ccctggagca
901 ccatgaatgc cacttcccaa acaagccact gccatcggcg ggcaccgtgc cctggctcca
961 gggtctcatc tgtaatgtga acaacacctg ctttccgcag ctgacaccgg gcgaggagcc
1021 cgggcgcctg agcaacttca acgactccct ggtctcccgg ctgctagccg atgcccgcac
1081 tgtgctggga ggggccagtg cccacaggac gctggctggc ctagggaagc tgatcgccac
1141 gctgagggct gcacgcagca cggcccagcc tcaaccaacc aagcagtctc cactggaacc
1201 acccatgctg gatgtcgcgg agctgctgac gtcactgctg cgcacggaat ccctggggtt
1261 ggcactgggc caagcccagg agcccttgca cagcttgttg gaggccgctg aggacctggc
1321 ccaggagctc ctggcgctgc gcagcctggt ggagcttcgg gcactgctgc agagaccccg
1381 agggaccagc ggccccctgg agttgctgtc agaggccctc tgcagtgtca ggggacctag
1441 cagcacagtg ggcccctccc tcaactggta cgaggctagt gacctgatgg agctggtggg
1501 gcaggagcca gaatccgccc tgccagacag cagcctgagc cccgcctgct cggagctgat
1561 tggagccctg gacagccacc cgctgtcccg cctgctctgg agacgcctga agcctctgat
1621 cctcgggaag ctactctttg caccagatac accttttacc cggaagctca tggcccaggt
1681 gaaccggacc ttcgaggagc tcaccctgct gagggatgtc cgggaggtgt gggagatgct
1741 gggaccccgg atcttcacct tcatgaacga cagttccaat gtggccatgc tgcagcggct
1801 cctgcagatg caggatgaag gaagaaggca gcccagacct ggaggccggg accacatgga
1861 ggccctgcga tcctttctgg accctgggag cggtggctac agctggcagg acgcacacgc
1921 tgatgtgggg cacctggtgg gcacgctggg ccgagtgacg gagtgcctgt ccttggacaa
1981 gctggaggcg gcaccctcag aggcagccct ggtgtcgcgg gccctgcaac tgctcgcgga
2041 acatcgattc tgggccggcg tcgtcttctt gggacctgag gactcttcag accccacaga
2101 gcacccaacc ccagacctgg gccccggcca cgtgcgcatc aaaatccgca tggacattga
2161 cgtggtcacg aggaccaata agatcaggga caggttttgg gaccctggcc cagccgcgga
2221 ccccctgacc gacctgcgct acgtgtgggg cggcttcgtg tacctgcaag acctggtgga
2281 gcgtgcagcc gtccgcgtgc tcagcggcgc caacccccgg gccggcctct acctgcagca
2341 gatgccctat ccgtgctatg tggacgacgt gttcctgcgt gtgctgagcc ggtcgctgcc
2401 gctcttcctg acgctggcct ggatctactc cgtgacactg acagtgaagg ccgtggtgcg
2461 ggagaaggag acgcggctgc gggacaccat gcgcgccatg gggctcagcc gcgcggtgct
2521 ctggctaggc tggttcctca gctgcctcgg gcccttcctg ctcagcgccg cactgctggt
2581 tctggtgctc aagctgggag acatcctccc ctacagccac ccgggcgtgg tcttcctgtt
2641 cttggcagcc ttcgcggtgg ccacggtgac ccagagcttc ctgctcagcg ccttcttctc
2701 ccgcgccaac ctggctgcgg cctgcggcgg cctggcctac ttctccctct acctgcccta
2761 cgtgctgtgt gtggcttggc gggaccggct gcccgcgggt ggccgcgtgg ccgcgagcct
2821 gctgtcgccc gtggccttcg gcttcggctg cgagagcctg gctctgctgg aggagcaggg
2881 cgagggcgcg cagtggcaca acgtgggcac ccggcctacg gcagacgtct tcagcctggc
2941 ccaggtctct ggccttctgc tgctggacgc ggcgctctac ggcctcgcca cctggtacct
3001 ggaagctgtg tgcccaggcc agtacgggat ccctgaacca tggaattttc cttttcggag
3061 gagctactgg tgcggacctc ggccccccaa gagtccagcc ccttgcccca ccccgctgga
3121 cccaaaggtg ctagtagaag aggcaccgcc cggcctgagt cctggcgtct ccgttcgcag
3181 cctggagaag cgctttcctg gaagcccgca gccagccctg cgggggctca gcctggactt
3241 ctaccagggc cacatcaccg ccttcctggg ccacaacggg gccggcaaga ccaccaccct
3301 gtccatcttg agtggcctct tcccacccag tggtggctct gccttcatcc tgggccacga
3361 cgtccgctcc agcatggccg ccatccggcc ccacctgggc gtctgtcctc agtacaacgt
3421 gctgtttgac atgctgaccg tggacgagca cgtctggttc tatgggcggc tgaaggtaag
3481 tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta taggatactt
3541 tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
3601 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
3661 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
3721 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
3781 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
3841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
3901 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3961 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4021 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4081 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4141 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4261 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4321 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4381 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4441 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4501 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
4921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
4981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
5881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
5941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6301 gttcctggcc ttttgctggc cttttgctca catgt
//
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