pAAVLINKv1-pCALM1-Cre-3'-MYO5A-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-MYO5A-FLAG                6611 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6611)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3312
                     /codon_start=1
                     /gene="MYO5A"
                     /gene_synonym="GS1; MYH12; MYO5; MYR12"
                     /product="unconventional myosin-Va isoform 3"
                     /label=MYO5A
                     /note="isoform 3 is encoded by transcript variant 
                     3;myosin-Va; myoxin; myosin V; myosin, heavy polypeptide 
                     kinase; myosin-12; dilute myosin heavy chain, non-muscle; 
                     unconventional myosin-Va; myosin VA (heavy chain 12, 
                     myoxin)"
                     /db_xref="GI:1835953126"
                     /db_xref="CCDS:CCDS92001.1"
                     /db_xref="GeneID:4644"
                     /db_xref="HGNC:HGNC:7602"
                     /db_xref="MIM:160777"
                     /protein_id="NP_001369276.1"
                     /translation="SQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGL
                     KETNRSSALDYHELNEDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSL
                     KEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLYFEELYADDPKKYQSYRISL
                     YKRMIDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIP
                     RKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDD
                     QKVRSLLTSTINSIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQ
                     NEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPT
                     GLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAITLNN
                     LLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDD
                     DAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMDA
                     KHIFPVTFPFNPSSLALETIQIPASLGLGFISRV"
     misc_feature    1796..1801
                     /label=SDS
     misc_feature    2189..2194
                     /label=SDS
     CDS             3319..3384
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3400..3831
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3544..3549
                     /label=SDS
     repeat_region   3874..4014
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3874..4003
                     /label=AAV2 ITR
     repeat_region   3875..4015
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4089..4544
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4826..4930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4931..5791
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5962..6550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtctcaact ggtgagccag
     1501 aaagaggcca tccaacccaa ggatgacaag aatacaatga cagattccac aatacttttg
     1561 gaagatgtac aaaaaatgaa agataaaggt gaaatagcac aagcatacat tggtttgaaa
     1621 gaaacaaata gatcatctgc tctggattac catgagttga atgaggatgg agagctgtgg
     1681 ctggtttatg aagggttaaa acaagccaac aggctcctgg aatcccagct gcagtcacag
     1741 aagaggagcc atgagaatga ggccgaggcc ctccgtgggg agatccagag cctgaaggag
     1801 gagaacaacc gacagcagca gctgctggcc cagaacctgc agctgccccc agaggcccgc
     1861 attgaggcca gcctgcagca cgagatcacc cggctgacca acgaaaactt gtattttgag
     1921 gaattatatg cagatgaccc taagaagtat caatcatatc ggatttccct ttacaaacgg
     1981 atgattgatt tgatggaaca acttgaaaaa caggataaga cggtccgtaa actgaaaaaa
     2041 caactgaaag tatttgccaa aaaaattggc gaactagaag tgggccagat ggagaacata
     2101 tccccaggac agatcattga tgaacccatc cgaccagtca acattcccag gaaagaaaag
     2161 gatttccaag ggatgctgga atacaagaag gaggatgagc aaaaacttgt taagaacctg
     2221 attctggaac tgaagccacg tggtgtagca gtcaatttga ttccaggatt accggcatat
     2281 atcctgttca tgtgtgttcg acatgctgac tacctgaatg atgatcagaa agtaaggtcg
     2341 ttgctaacat caacaattaa cagcatcaaa aaagtattga agaaaagagg tgatgatttt
     2401 gaaaccgtct ccttctggct ctctaacaca tgccgatttt tgcactgctt gaaacagtac
     2461 agtggagaag agggctttat gaagcacaac acatctcgcc agaatgaaca ctgcctcacc
     2521 aattttgacc tggctgagta tcggcaggtg ctgagtgact tggccattca gatctaccag
     2581 cagctcgtgc gggtgttaga gaacatcctt cagccaatga ttgtctcagg catgctggaa
     2641 catgaaacga ttcagggcgt gtctggggtg aagcccacag ggttgagaaa gcgaacctcc
     2701 agtatcgccg atgagggcac ctacacactg gactccatcc tccggcagct caactccttc
     2761 cactcggtca tgtgtcagca tggcatggac cctgaactga tcaagcaggt ggtcaagcag
     2821 atgttctaca tcataggggc catcaccctg aacaaccttc tcctgcggaa ggacatgtgc
     2881 tcctggagta aaggcatgca gatcaggtac aatgtcagtc aactggaaga atggctgcgt
     2941 gacaagaatc tgatgaatag tggggctaaa gaaaccctgg aacctctcat tcaggctgct
     3001 caacttttgc aagtgaaaaa gaaaacagat gatgatgcag aagccatttg ttctatgtgc
     3061 aatgctttaa ctactgccca gattgtgaaa gtgttgaatt tgtatactcc agttaatgag
     3121 tttgaagaaa gagtctctgt gtcgttcatt cgtactatac agatgcgttt acgagacagg
     3181 aaagactctc cccagctgct catggatgct aaacacatct ttcctgtcac ctttcctttc
     3241 aacccatctt ccctcgcact agaaaccatc cagattccag ccagcctcgg cctgggcttc
     3301 atttcacggg tctgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
     3361 gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat caacctctgg
     3421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3541 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
     3601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
     3661 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
     3721 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3781 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
     3841 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     3901 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3961 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4021 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4081 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4141 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4381 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     4441 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     4501 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4561 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6601 tgctcacatg t
//