pEcCpf1H

LOCUS       pEcCpf1H               14510 bp    DNA     circular SYN 03-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2025 from SnapGene 8.1.0
            https://www.biofeng.com
COMMENT     Sequence Label: pEcCpf1H
FEATURES             Location/Qualifiers
     source          1..14510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(107..1057)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with 
                     thepSC101 origin"
                     /label=rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(1105..1327)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(1832..2647)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7027..7905)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        7932..8216
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; 
                     thearaC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             8260..8676
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in 
                     thelambda Red system"
                     /label=gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             9495..10175
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in 
                     thelambda Red system"
                     /label=exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             10757..12178
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders 
                     bacterialgrowth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     misc_RNA        complement(12301..12376)
                     /label=g RNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(12476..12594)
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, 
                     conferringtight induction with L-rhamnose and repression 
                     with D-glucose in the presence of RhaR and RhaS (Giacalone 
                     et al., 2006)"
     CDS             12740..13576
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter"
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQRYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     CDS             13650..14498
                     /codon_start=1
                     /gene="E. coli rhaR"
                     /product="transcriptional activator of rhaR and rhaS"
                     /label=rhaR
                     /note="needed for rhamnose-dependent activation of the rhaB
                     promoter"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIVW
                     RGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQGAI
                     PGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLNRHR
                     YTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTGMTIN
                     QYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQK
                     D"
ORIGIN
        1 catgggtatg gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt
       61 tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt
      121 atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac
      181 cattgagatc atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct
      241 gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat
      301 ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt
      361 cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc
      421 ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg
      481 gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca agcattaaca
      541 tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta
      601 gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat
      661 gttccagatt atattttatg aattttttta actggaaaag ataaggcaat atctcttcac
      721 taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct
      781 caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg
      841 ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt
      901 tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta
      961 gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg
     1021 ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat
     1081 tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt
     1141 gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta
     1201 gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta
     1261 taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt
     1321 ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg
     1381 ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg
     1441 ttgcggccgc aatcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     1501 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     1561 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     1621 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     1681 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     1741 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     1801 cccactgcag caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     1861 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     1921 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     1981 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     2041 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat
     2101 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     2161 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     2221 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     2281 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     2341 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     2401 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     2461 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2521 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     2581 aatcagcatc catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat
     2641 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg
     2701 atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc
     2761 tgcagctgaa caatacttaa tactatagaa tgataacaaa ataaactact ttttaaaaga
     2821 attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga agagatattt
     2881 tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa ctattattga
     2941 aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc aaaaatgtca
     3001 atttatcaag aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc
     3061 ccacagggta aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa
     3121 agagctaaag actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata
     3181 gaggagatat taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt
     3241 tattttaaac ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa
     3301 gatacgataa agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg
     3361 tttaatcaaa accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta
     3421 aagcaatcta aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata
     3481 gatgaggcgt tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt
     3541 catgaaaata gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg
     3601 atagtagatg ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa
     3661 gacaaagctc cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta
     3721 acctttgata ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa
     3781 gtttttgaga tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat
     3841 actattattg gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa
     3901 tatataaatc tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt
     3961 gttttattta agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta
     4021 gaagatgata gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt
     4081 aaaacagtag aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa
     4141 gctcaaaaac ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta
     4201 tcacaacaag tttttgatga ttatagtgtt attggtacag cggtactaga atatataact
     4261 caacaaatag cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc
     4321 aaaaaaactg aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa
     4381 tttaataagc atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt
     4441 gcggctattc cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata
     4501 tctatcaaat atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat
     4561 gttaaagcta tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata
     4621 tttcatatta gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat
     4681 ctagtatttg aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt
     4741 agaaactata taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac
     4801 tcgactttgg ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt
     4861 atcaaagatg ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat
     4921 gataaagcta tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta
     4981 cctggcgcaa ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat
     5041 aatcctagtg aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt
     5101 cctcaaaaag gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat
     5161 ttttataaac agtctataag taagcatccg gagtggaaag attttggatt tagattttct
     5221 gatactcaaa gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac
     5281 aaactaactt ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa
     5341 ttgtacctat tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat
     5401 ctacatactt tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat
     5461 aagctaaatg gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact
     5521 cacccagcta aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt
     5581 tttgaatatg atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt
     5641 cctattacaa tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta
     5701 ttgctaaaag aaaaagcaaa tgatgttcat atattaagta tagatagagg tgaaagacat
     5761 ttagcttact atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac
     5821 atcattggta atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa
     5881 gatagggatt cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag
     5941 ggctatctat ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt
     6001 gtggtttttg aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag
     6061 gtctatcaaa agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat
     6121 aatgagtttg ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag
     6181 acttttaaaa agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact
     6241 tcaaaaattt gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc
     6301 agcaaatctc aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc
     6361 tattttgagt ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg
     6421 actatagcta gctttgggag tagattgatt aactttagaa attcagataa aaatcataat
     6481 tgggatactc gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct
     6541 atcgaatatg ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag
     6601 ttttttgcta agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca
     6661 ggtactgagt tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat
     6721 tcgcgacagg cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt
     6781 gggctaaaag gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc
     6841 aatttggtta tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaaagt
     6901 atattttaga tgaagattat ttcttaatct agacatgagc ggatacatat ttgaatgtat
     6961 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcatc
     7021 gatttattat gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa
     7081 ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaagtaga gttgatcgtc
     7141 aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt
     7201 cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg
     7261 gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc
     7321 aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc
     7381 catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag
     7441 cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg
     7501 cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt
     7561 ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac
     7621 ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg
     7681 cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac
     7741 cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat
     7801 aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt
     7861 aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc
     7921 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
     7981 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     8041 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     8101 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     8161 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     8221 gtttttttgg gaattcgagc tctaaggagg ttataaaaaa tggatattaa tactgaaact
     8281 gagatcaagc aaaagcattc actaaccccc tttcctgttt tcctaatcag cccggcattt
     8341 cgcgggcgat attttcacag ctatttcagg agttcagcca tgaacgctta ttacattcag
     8401 gatcgtcttg aggctcagag ctgggcgcgt cactaccagc agctcgcccg tgaagagaaa
     8461 gaggcagaac tggcagacga catggaaaaa ggcctgcccc agcacctgtt tgaatcgcta
     8521 tgcatcgatc atttgcaacg ccacggggcc agcaaaaaat ccattacccg tgcgtttgat
     8581 gacgatgttg agtttcagga gcgcatggca gaacacatcc ggtacatggt tgaaaccatt
     8641 gctcaccacc aggttgatat tgattcagag gtataaaacg aatgaaccaa atcgtgaagt
     8701 tcactgacga ctctggcctg gcggttcaag ttactccaga cgatgttcgc cgttatatct
     8761 gtgagaacgc tactgaaaaa gaggtgggcc tctttctgca actctgtcag actcaacgtc
     8821 tgaatccgtt tgtgaaagac gcttacctgg tgaaatacgg cggtgctcca gcttctatga
     8881 ttacttccta tcaagttttt aaccgtcgcg cgtgtcgtga tgctaactat gatggtatca
     8941 aatctggtgt ggttgttctg cgtgacggtg atgttgtgca taaacgtggt gctgcgtgct
     9001 acaaaaaggc gggtgaggag ctcatcggtg gttgggcgga agttcgcttt aaggatggcc
     9061 gcgagactgc gtatgctgag gtggcgctcg acgactattc caccggcaaa tctaattggg
     9121 cgaaaatgcc gggtgttatg atcgaaaaat gcgcgaaggc tgctgcttgg cgcctcgcgt
     9181 tcccggacac ttttcagggc atgtacgctg cggaggaaat ggatcaagcg caacagccag
     9241 aacaggtgcg cgctcaggcg gagcaaccag tggatctcca gccaatccgc gaactcttca
     9301 agccatattg cgaacacttc ggcatcactc cggctgaggg tatgactgct gtttgtggtg
     9361 cggtgggcgc tgaaggcatg cactctatga ccgagcagca agctcgccgt gctcgcgctt
     9421 ggatggagga agaaatggct gcgccagctg tggaagcgga gtatgaggtt gttgacgaag
     9481 gcgaggtgtt ttaaatgaca ccggacatta tcctgcagcg taccgggatc gatgtgagag
     9541 ctgtcgaaca gggggatgat gcgtggcaca aattacggct cggcgtcatc accgcttcag
     9601 aagttcacaa cgtgatagca aaaccccgct ccggaaagaa gtggcctgac atgaaaatgt
     9661 cctacttcca caccctgctt gctgaggttt gcaccggtgt ggctccggaa gttaacgcta
     9721 aagcactggc ctggggaaaa cagtacgaga acgacgccag aaccctgttt gaattcactt
     9781 ccggcgtgaa tgttactgaa tccccgatca tctatcgcga cgaaagtatg cgtaccgcct
     9841 gctctcccga tggtttatgc agtgacggca acggccttga actgaaatgc ccgtttacct
     9901 cccgggattt catgaagttc cggctcggtg gtttcgaggc cataaagtca gcttacatgg
     9961 cccaggtgca gtacagcatg tgggtgacgc gaaaaaatgc ctggtacttt gccaactatg
    10021 acccgcgtat gaagcgtgaa ggcctgcatt atgtcgtgat tgagcgggat gaaaagtaca
    10081 tggcgagttt tgacgagatc gtgccggagt tcatcgaaaa aatggacgag gcactggctg
    10141 aaattggttt tgtatttggg gagcaatggc gatgacgcat cctcacgata atatccgggt
    10201 aggcgcaatc actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc
    10261 tgttatccga aatccactga aagcacagcg gctggctgag gagataaata ataaacgagg
    10321 ggctgtatgc acaaagcatc ttctgttgag ttaagaacga gtatcgagat ggcacatagc
    10381 cttgctcaaa ttggaatcag gtttgtgcca ataccagtag aaacagacga agaatccatg
    10441 ggtatggaca gatcctcttt aggcccgtag tctgcaaatc cttttatgat tttctatcaa
    10501 acaaaagagg aaaatagacc agttgcaatc caaacgagag tctaatagaa tgaggtcgaa
    10561 aagtaaatcg cgcgggtttg ttactgataa agcaggcaag acctaaaatg tgtaaagggc
    10621 aaagtgtata ctttggcgtc accccttaca tattttaggt ctttttttat tgtgcgtaac
    10681 taacttgcca tcttcaaaca ggagggctgg aagaagcaga ccgctaacac agtacataaa
    10741 aaaggagaca tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag tattaacctt
    10801 tactaccgca ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa cgaaccaaaa
    10861 gccatataag gaaacatacg gcatttccca tattacacgc catgatatgc tgcaaatccc
    10921 tgaacagcaa aaaaatgaaa aatatcaagt tcctgaattc gattcgtcca caattaaaaa
    10981 tatctcttct gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa acgctgacgg
    11041 cactgtcgca aactatcacg gctaccacat cgtctttgca ttagccggag atcctaaaaa
    11101 tgcggatgac acatcgattt acatgttcta tcaaaaagtc ggcgaaactt ctattgacag
    11161 ctggaaaaac gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa atgattctat
    11221 cctaaaagac caaacacaag aatggtcagg ttcagccaca tttacatctg acggaaaaat
    11281 ccgtttattc tacactgatt tctccggtaa acattacggc aaacaaacac tgacaactgc
    11341 acaagttaac gtatcagcat cagacagctc tttgaacatc aacggtgtag aggattataa
    11401 atcaatcttt gacggtgacg gaaaaacgta tcaaaatgta cagcagttca tcgatgaagg
    11461 caactacagc tcaggcgaca accatacgct gagagatcct cactacgtag aagataaagg
    11521 ccacaaatac ttagtatttg aagcaaacac tggaactgaa gatggctacc aaggcgaaga
    11581 atctttattt aacaaagcat actatggcaa aagcacatca ttcttccgtc aagaaagtca
    11641 aaaacttctg caaagcgata aaaaacgcac ggctgagtta gcaaacggcg ctctcggtat
    11701 gattgagcta aacgatgatt acacactgaa aaaagtgatg aaaccgctga ttgcatctaa
    11761 cacagtaaca gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca aatggtacct
    11821 gttcactgac tcccgcggat caaaaatgac gattgacggc attacgtcta acgatattta
    11881 catgcttggt tatgtttcta attctttaac tggcccatac aagccgctga acaaaactgg
    11941 ccttgtgtta aaaatggatc ttgatcctaa cgatgtaacc tttacttact cacacttcgc
    12001 tgtacctcaa gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa acagaggatt
    12061 ctacgcagac aaacaatcaa cgtttgcgcc aagcttcctg ctgaacatca aaggcaagaa
    12121 aacatctgtt gtcaaagaca gcatccttga acaaggacaa ttaacagtta acaaataaaa
    12181 acgcaaaaga aaatgccgat atcctattgg cattttcttt tatttcttat caacataaag
    12241 gtgaatccca tatgaactat ataaaagcag gcaaatggct aaccgtattc ctcaaaaaaa
    12301 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
    12361 tttctagctc taaaacggtt ggactcaaga cgatagtatc tacaacagta gaaatttggc
    12421 ttcagcagag cgcagataca tctacaacag tagaaattat ttaaagttct tagactacga
    12481 ccagtctaaa aagcgcctga attcgcgacc ttctcgttac tgacaggaaa atgggccatt
    12541 ggcaaccagg gaaagatgaa cgtgatgatg ttcacaattt gctgaattgt ggtgatgtga
    12601 tgctcaccgc atttcctgaa aattcacgct gtatcttgaa aaatcgacgt tttttacgtg
    12661 gttttccgtc gaaaatttaa ggtaagaacc tgacctcgtg attactattt cgccgtgttg
    12721 acgacatcag gaggccagta tgaccgtatt acatagtgtg gatttttttc cgtctggtaa
    12781 cgcgtccgtg gcgatagaac cccggctccc gcaggcggat tttcctgaac atcatcatga
    12841 ttttcatgaa attgtgattg tcgaacatgg cacgggtatt catgtgttta atgggcagcc
    12901 ctataccatc accggtggca cggtctgttt cgtacgcgat catgatcggc atctgtatga
    12961 acataccgat aatctgtgtc tgaccaatgt gctgtatcgc tcgccggatc gatttcagtt
    13021 tctcgccggg ctgaatcagt tgctgccaca agagctggat gggcagtatc cgtctcactg
    13081 gcgcgttaac cacagcgtat tgcagcaggt gcgacagctg gttgcacaga tggaacagca
    13141 ggaaggggaa aatgatttac cctcgaccgc cagtcgcgag atcttgttta tgcaattact
    13201 gctcttgctg cgtaaaagca gtttgcagga gaacctggaa aacagcgcat cacgtctcaa
    13261 cttgcttctg gcctggctgg aggaccattt tgccgatgag gtgaattggg atgccgtggc
    13321 ggatcaattt tctctttcac tgcgtacgct acatcggcag cttaagcagc aaacgggact
    13381 gacgcctcag cgatacctga accgcctgcg actgatgaaa gcccgacatc tgctacgcca
    13441 cagcgaggcc agcgttactg acatcgccta tcgctgtgga ttcagcgaca gtaaccactt
    13501 ttcgacgctt tttcgccgag agtttaactg gtcaccgcgt gatattcgcc agggacggga
    13561 tggctttctg caataacgcg aatcttctca acgtatttgt acgccatatt gcgaataatc
    13621 aacttcgttc tctggccgag gtagccacgg tggcgcatca gttaaaactt ctcaaagatg
    13681 atttttttgc cagcgaccag caggcagtcg ctgtggctga ccgttatccg caagatgtct
    13741 ttgctgaaca tacacatgat ttttgtgagc tggtgattgt ctggcgcggt aatggcctgc
    13801 atgtactcaa cgatcgccct tatcgcatta cccgtggcga tctcttttac attcatgctg
    13861 acgataaaca ctcctacgct tccgttaacg atctggtttt gcagaatatt atttattgcc
    13921 cggagcgtct gaagctgaat cttgactggc agggggcgat tccgggattt aacgccagcg
    13981 cagggcaacc acactggcgc ttaggtagca tggggatggc gcaggcgcgg caggttatcg
    14041 gtcagcttga gcatgaaagt agtcagcatg tgccgtttgc taacgaaatg gctgagttgc
    14101 tgttcgggca gttggtgatg ttgctgaatc gccatcgtta caccagtgat tcgttgccgc
    14161 caacatccag cgaaacgttg ctggataagc tgattacccg gctggcggct agcctgaaaa
    14221 gtccctttgc gctggataaa ttttgtgatg aggcatcgtg cagtgagcgc gttttgcgtc
    14281 agcaatttcg ccagcagact ggaatgacca tcaatcaata tctgcgacag gtcagagtgt
    14341 gtcatgcgca atatcttctc cagcatagcc gcctgttaat cagtgatatt tcgaccgaat
    14401 gtggctttga agatagtaac tatttttcgg tggtgtttac ccgggaaacc gggatgacgc
    14461 ccagccagtg gcgtcatctc aattcgcaga aagattaatt cgccatgccc
//