| 质粒名称: | pAAV-CAG-BR1-P2A-FusionRed |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000233 |
| 产品用途: | Expresses BR1 and FusionRed in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pAAV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CAG
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | BR1, FusionRed |
| 物种来源: | Arabidopsis thaliana |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Liang G., Lai C., Yue Z., Zhang H., Li D., Chen Z., Lu X., Tao L., Subach F.V., Piatkevich K.D.
Front. Bioeng. Biotechnol. 10, 1039317 (2022)
|
pAAV-CAG-BR1-P2A-FusionRed
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-BR1-P2A-FusionRed载体质粒基本信息
pAAV-CAG-BR1-P2A-FusionRed质粒图谱载体图谱和pAAV-CAG-BR1-P2A-FusionRed载体序列质粒序列多克隆位点信息
pAAV-CAG-BR1-P2A-FusionRed质粒载体简介
pAAV-CAG-BR1-P2A-FusionRed质粒序列载体序列
LOCUS pAAV-CAG-BR1-P2A-FusionRed 5848 bp DNA circular SYN 31-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5848)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5848
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 675..699
/label=Primer 1-F
misc_feature 977..982
/label=Kozak sequence
misc_feature 983..1315
/label=BR1
CDS 1340..1396
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1409..2104
/codon_start=1
/product="monomeric red fluorescent protein optimized for
use as a fusion tag (Shemiakina et al., 2012)"
/label=FusionRed
/note="mammalian codon-optimized"
/translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
STGGAGDGGK"
primer_bind complement(1656..1680)
/label=Primer 1-R
misc_feature 2132..2720
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2603..2614)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2763..2884
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 3046..3175
/label=AAV2 ITR
rep_origin complement(3412..4000)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4171..5031)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5032..5136)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5162..5617)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 5689..5818
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatgatcga gaagaactac gtgatcaccg accccagact
1021 gcccgacaac cccatcatct tcgccagcga cggcttcctg gagctgaccg gctacagcag
1081 agaggagatc ctgggcagaa acgccagatt cctgcagggc cccgagaccg accaggccac
1141 cgtgcagaag atcagagacg ccatcagaga ccagagagag accaccgtgc agctgatcaa
1201 ctacaccaag agcggcagaa agttctggaa cctgctgcac ctgcagcccg tgagagacca
1261 gaagggcgag ctgcagtact tcatcggcgt gcagctggac ggcagcgaca gagtgcgacc
1321 ggttcacgtg ggaagtgggg ctacaaactt ctcactgctg aaacaggccg gggatgtgga
1381 ggaaaaccca ggaccacacg tgactagtat ggtgagcgag ctgattaagg agaacatgcc
1441 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
1501 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtcgtcg agggcggccc
1561 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
1621 caagcaccct ccgggcatcc ccgacttctt taagcagtcc ttccctgagg gcttcacatg
1681 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
1741 ccaggacggc tgcctcatct acaacgtcaa ggttagaggg gtgaacttcc cagccaacgg
1801 ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc accgagacga tgtaccctgc
1861 tgacggagga ctggaaggcg catgtgacat ggctctgaag ctcgtgggag gaggacacct
1921 gatctgcaac cttgagacaa catacagatc caagaaaccc gctacgaacc tcaagatgcc
1981 cggcgtctac aacgtggacc acagactgga aagaatcaag gaggccgacg atgagacata
2041 cgtcgagcag cacgaggtgg ctgtggccag atactctact ggtggcgctg gtgatggagg
2101 taaataagaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
2161 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
2221 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
2281 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
2341 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
2401 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
2461 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
2521 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
2581 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
2641 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
2701 cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc gagcagacat
2761 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
2821 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
2881 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
2941 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ttcctagagc
3001 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
3061 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
3121 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgca
3181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
3601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
3841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
3961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
4021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
4081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
4141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
4201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
4261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
4321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
4381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
4441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
4501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
4561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
4621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
4681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
4741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
4801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
4861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
4921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
4981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
5041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
5101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
5161 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
5221 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
5281 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
5341 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
5401 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
5461 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
5521 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
5581 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
5641 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct gcgcgctcgc
5701 tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc
5761 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttccttg
5821 tagttaatga ttaacccgcc atgctact
//
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