| 质粒名称: | pAAVLINK-EF1a-HA-5'-NCOR1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001717 |
| 产品用途: | 5' AAVLINK plamid for NCOR1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NCOR1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-NCOR1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-NCOR1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-NCOR1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-NCOR1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-NCOR1质粒载体简介
pAAVLINK-EF1a-HA-5'-NCOR1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-NCOR1 7109 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7109)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7109
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4249
/codon_start=1
/label=NCOR1
/translation="MSSSGYPPNQGAFSTEQSRYPPHSVQYTFPNTRHQQEFAVPDYRS
SHLEVSQASQLLQQQQQQQLRRRPSLLSEFHPGSDRPQERRTSYEPFHPGPSPVDHDSL
ESKRPRLEQVSDSHFQRVSAAVLPLVHPLPEGLRASADAKKDPAFGGKHEAPSSPISGQ
PCGDDQNASPSKLSKEELIQSMDRVDREIAKVEQQILKLKKKQQQLEEEAAKPPEPEKP
VSPPPVEQKHRSIVQIIYDENRKKAEEAHKIFEGLGPKVELPLYNQPSDTKVYHENIKT
NQVMRKKLILFFKRRNHARKQREQKICQRYDQLMEAWEKKVDRIENNPRRKAKESKTRE
YYEKQFPEIRKQREQQERFQRVGQRGAGLSATIARSEHEISEIIDGLSEQENNEKQMRQ
LSVIPPMMFDAEQRRVKFINMNGLMEDPMKVYKDRQFMNVWTDHEKEIFKDKFIQHPKN
FGLIASYLERKSVPDCVLYYYLTKKNENYKALVRRNYGKRRGRNQQIARPSQEEKVEEK
EEDKAEKTEKKEEEKKDEEEKDEKEDSKENTKEKDKIDGTAEETEEREQATPRGRKTAN
SQGRRKGRITRSMTNEAAAASAAAAAATEEPPPPLPPPPEPISTEPVETSRWTEEEMEV
AKKGLVEHGRNWAAIAKMVGTKSEAQCKNFYFNYKRRHNLDNLLQQHKQKTSRKPREER
DVSQCESVASTVSAQEDEDIEASNEEENPEDSEVEAVKPSEDSPENATSRGNTEPAVEL
EPTTETAPSTSPSLAVPSTKPAEDESVETQVNDSISAETAEQMDVDQQEHSAEEGSVCD
PPPATKADSVDVEVRVPENHASKVEGDNTKERDLDRASEKVEPRDEDLVVAQQINAQRP
EPQSDNDSSATCSADEDVDGEPERQRMFPMDSKPSLLNPTGSILVSSPLKPNPLDLPQL
QHRAAVIPPMVSCTPCNIPIGTPVSGYALYQRHIKAMHESALLEEQRQRQEQIDLECRS
STSPCGTSKSPNREWEVLQPAPHQVITNLPEGVRLPTTRPTRPPPPLIPSSKTTVASEK
PSFIMGGSISQGTPGTYLTSHNQASYTQETPKPSVGSISLGLPRQQESAKSATLPYIKQ
EEFSPRSQNSQPEGLLVRAQHEGVVRGTAGAIQEGSITRGTPTSKISVESIPSLRGSIT
Q"
misc_feature 809..814
/label=SDS
misc_feature 1394..1399
/label=SDS
misc_feature 1421..1426
/label=SDS
misc_feature 1424..1429
/label=SDS
misc_feature 2874..2879
/label=SDS
misc_feature 3042..3047
/label=SDS
intron 4250..4290
/label=Splicing Donor
misc_feature 4294..4327
/label=lox2272-JT1571
repeat_region 4372..4512
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4372..4501
/label=AAV2 ITR
rep_origin 4587..5042
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5324..5428
/gene="bla"
/label=AmpR promoter
CDS 5429..6289
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6460..7048
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat gtcaagttca ggttatcctc ccaaccaagg
781 agcattcagc acagaacaaa gtcgttatcc tcctcactct gtccagtata catttcccaa
841 cacccgccac cagcaggagt tcgcagtccc tgattatcgt tcctctcatc ttgaagtgag
901 tcaggcatca cagcttttgc agcaacagca gcagcaacag cttcgaaggc gaccttcctt
961 gctttcagaa tttcacccag gttctgacag gcctcaagaa aggagaacta gttatgaacc
1021 gtttcatcca ggcccatccc cagtggatca tgattcactg gaatcgaagc gaccacgtct
1081 ggaacaggtt tctgattctc attttcagcg tgtcagtgct gcggttttgc ctttagtgca
1141 cccgctgcca gaagggctga gggcttctgc agatgctaag aaggatccag cattcggagg
1201 caaacatgaa gctccatcct ctccaatttc ggggcaacca tgtggagatg atcaaaatgc
1261 ttcaccttca aaactctcaa aggaagagtt aatacagagt atggatcgtg tagatcgaga
1321 aattgcaaaa gtagaacagc agatccttaa actgaaaaag aaacaacaac agcttgaaga
1381 agaggcagct aaacctcctg agcctgagaa gcccgtgtcc cctcctcctg tggagcagaa
1441 acaccgcagt attgtccaaa ttatttatga tgagaatcgg aaaaaagcag aagaagctca
1501 taaaattttt gaaggtcttg gcccaaaagt tgaactgcca ctgtataacc agccatcaga
1561 taccaaggtg taccatgaga acatcaagac aaaccaggtg atgaggaaaa aactcatttt
1621 attttttaaa agaagaaatc atgcaagaaa acaaagggaa caaaaaatct gccagcgtta
1681 tgatcagctc atggaggcat gggagaaaaa agtggacaga atagaaaata atcctcggag
1741 gaaagctaaa gaaagcaaaa caagggaata ctatgaaaag cagtttccag aaattcgaaa
1801 acaaagagaa cagcaagaaa gatttcagcg agttgggcag aggggagctg gtctttcagc
1861 caccattgct aggagtgagc atgagatttc tgaaattatt gatgggctct ctgagcagga
1921 gaataatgag aaacaaatgc ggcagctctc tgtgattcca cctatgatgt ttgatgcaga
1981 acaaagacga gtcaagttca ttaacatgaa tgggcttatg gaggacccta tgaaagtgta
2041 taaagatagg cagtttatga atgtttggac tgaccatgaa aaggagatct ttaaggacaa
2101 gtttatccag catccaaaaa actttggact aattgcatca tacttggaga ggaagagtgt
2161 tcctgattgt gttttgtatt actatttaac caagaaaaat gagaattata aagccctcgt
2221 cagaaggaat tatgggaaac gcagaggcag aaaccagcaa attgctcgac cctcgcaaga
2281 agaaaaagta gaagaaaaag aagaggataa agcagaaaaa acagaaaaaa aagaagaaga
2341 aaagaaagat gaagaggaaa aagatgaaaa agaagactcc aaagaaaata ccaaggaaaa
2401 ggacaagata gatggtacag cagaagaaac tgaggaaaga gagcaagcca caccccgggg
2461 gcgaaagact gccaacagtc agggccgccg taagggccgg atcaccaggt ccatgacaaa
2521 cgaagctgca gctgccagtg ctgcagccgc agcggctact gaagagcccc caccacctct
2581 gccaccgcca ccagaaccca tttctacaga gcctgtggag acctctcgat ggacagaaga
2641 agaaatggaa gttgctaaaa aaggtctagt agaacatggt cgtaactggg cagcaattgc
2701 taaaatggtg ggaacgaaaa gtgaagctca atgtaaaaac ttctatttta actataaaag
2761 gcgacacaat cttgacaacc tcttacagca gcataaacag aaaacttcac gaaaacctcg
2821 tgaagagcga gatgtgtctc aatgtgaaag tgtcgcttcc actgtttctg ctcaggagga
2881 tgaagatatt gaagcctcca atgaagaaga aaatccagaa gacagcgaag ttgaagctgt
2941 caagcccagc gaggacagtc ctgaaaatgc tacttctcga ggaaacacag aacctgcggt
3001 tgagcttgag cccaccacgg aaactgcacc cagtacatct ccctccttag cagttccaag
3061 tacaaaacca gctgaagatg aaagtgtgga gacccaggtg aatgacagca tcagtgctga
3121 gacagcagag cagatggatg tagatcagca ggagcacagt gctgaagagg gttctgtttg
3181 tgatccccca cccgctacca aagctgactc tgtggacgtt gaagtgaggg tgccagaaaa
3241 ccatgcatct aaagttgaag gtgataatac caaagaaaga gacttggata gagccagtga
3301 gaaggtggaa cctagagatg aagatttggt ggtagctcag caaataaatg cccaaaggcc
3361 cgagccccag tcagacaatg attccagtgc cacgtgcagc gctgatgagg atgtggatgg
3421 agagccagag aggcagagaa tgtttcctat ggactcaaag ccttcactgt taaaccccac
3481 tggatctata ctcgtctcat ctccgttaaa accaaatcca ctggatctgc cacagcttca
3541 gcatcgagct gctgttatcc caccaatggt atcctgcacc ccatgtaaca taccaattgg
3601 aaccccagtg agcggctatg ctctctacca gcgacacatt aaagcaatgc atgagtcagc
3661 actcctggag gagcagcggc agagacaaga acagatagat ttggaatgta gaagttctac
3721 aagtccatgt ggcacatcca agagtccaaa cagagagtgg gaagtccttc agcctgctcc
3781 acatcaagtg ataactaatc tccctgaagg cgttcggctt ccgacaactc gaccaaccag
3841 gccaccgccc cctctcatcc cgtcatccaa aaccacagtg gcttcagaaa aaccatcttt
3901 tataatggga ggctccatct cacagggaac accaggcact tatttgactt ctcataatca
3961 ggcttcctac actcaagaaa cacccaagcc gtcagtggga tctatctctc ttggactgcc
4021 acggcaacag gaatctgcca aatcagctac tttgccctac atcaagcagg aagaattttc
4081 tccccgaagc caaaactcac aacctgaggg tctgttggtc agggcccaac atgaaggtgt
4141 agtcagaggt accgcaggag ccatacaaga aggaagtata actcggggaa ctccaaccag
4201 caaaatttca gtggagagca ttccatccct acggggctct atcactcagg taagtatcaa
4261 ggttacaaga caggtttaag gagaccaata agctattatt cgtataggat actttatacg
4321 aagttattaa gttatggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
4381 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
4441 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
4501 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
4561 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
4621 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
4681 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4741 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4801 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4861 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
4921 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
4981 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
5041 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
5101 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
5161 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
5221 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
5281 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
5341 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
5401 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
5461 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
5521 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
5581 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
5641 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
5701 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
5761 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
5821 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
5881 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5941 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
6001 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
6061 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
6121 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
6181 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
6241 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
6301 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
6361 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
6421 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
6481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
6541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
6601 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
6661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
6781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6841 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
7021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
7081 ggccttttgc tggccttttg ctcacatgt
//
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