| 质粒名称: | pMV306hsp+Lux |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26159 |
| 存储实验室: | Brian Robertson, Siouxsie Wiles |
|
载体骨架基本信息 |
|
| 载体名称: | pMV306hsp |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4373 bp |
| 完整质粒大小: | 10095 bp |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Bacterial luciferase operon |
| 物种来源: | Photorhabdus luminescens |
| 插入大小: | 5722 bp |
| 基因又名: | LuxABCDE |
| 基因突变: | It contains a gram-positive enhanced translation signal in front of luxA, luxC and luxE |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRV (未破坏) |
| 5' 测序引物 | agaataacgttggcactcgc |
|
文献引用方法 |
|
| 材料和方法部分: | pMV306hsp+Lux was a gift from Brian Robertson & Siouxsie Wiles (爱迪基因plasmid # 26159) |
| 参考文献部分: | Optimisation of bioluminescent reporters for use with mycobacteria. Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S. PLoS One. 2010 May 24;5(5):e10777. 10.1371/journal.pone.0010777 PubMed 20520722 |
买家导航
pMV306hsp+Lux载体质粒基本信息
pMV306hsp+Lux质粒图谱载体图谱和pMV306hsp+Lux载体序列质粒序列多克隆位点信息
pMV306hsp+Lux质粒载体简介
pMV306hsp+Lux质粒序列载体序列
LOCUS pMV306hsp+Lux 10087 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMV306hsp+Lux
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10087)
AUTHORS Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J,
Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S
TITLE Optimisation of bioluminescent reporters for use with mycobacteria.
JOURNAL PLoS One. 2010 May 24;5(5):e10777.
PUBMED 20520722
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pMV306hsp+Lux|
FEATURES Location/Qualifiers
source 1..10087
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1896..1915)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 1907..2722
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(1980..1999)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
rep_origin 3054..3642
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3543..3562
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 4321..5403
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 5418..6401
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
CDS 6425..7867
/codon_start=1
/gene="luxC"
/product="LuxC fatty acid reductase"
/label=LuxC
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
DVAVEIEQTRFLEEDKFLVFVP"
CDS 7879..8802
/codon_start=1
/gene="luxD"
/product="LuxD acyltransferase"
/label=LuxD
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
FEHLTIATVNERRMRIEIENQAISLS"
CDS 8828..9940
/codon_start=1
/gene="luxE"
/product="LuxE"
/label=LuxE
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
terminator 10005..10051
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag
1801 cgacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
1861 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
1921 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
1981 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
2041 gaagccccat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
2101 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
2161 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
2221 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
2281 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
2341 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
2401 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataatct
2461 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
2521 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
2581 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
2641 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
2701 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
2761 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
2821 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
2881 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
2941 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
3001 cctcactagt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
3181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
3421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
3781 cgaacgaccg agcgcaacgc gtgcggccgc tctagaggtg accacaacga cgcgcccgct
3841 ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg ttgtcgttgg cggtcatggg
3901 ccgaacatac tcacccggat cggagggccg aggacaaggt cgaacgaggg gcatgacccg
3961 gtgcggggct tcttgcactc ggcataggcg agtgctaaga ataacgttgg cactcgcgac
4021 cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg tcgcagcgag tggcagcgag
4081 gacaacttga gccgtccgtc gcgggcactg cgcccggcca gcgtaagtag cggggttgcc
4141 gtcacccggt gacccccggt ttcatccccg atccggagga atcacttcgc aatggccaag
4201 acaattgcgg atcccccggg ctgcaggaat tcgaatggcc atgggacgtc gacctgaggt
4261 aattataacc cgggccctat atatggatcc aattgcaatg atcgtcgaca ggaggactct
4321 atgaaatttg gaaacttttt gcttacatac caacctcccc aattttctca aacagaggta
4381 atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg
4441 ttactggagc atcatttcac ggagtttggt ttgcttggta acccttatgt cgctgctgca
4501 tatttacttg gcgcgactaa aaaattgaat gtaggaactg ccgctattgt tcttcccaca
4561 gcccatccag tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga
4621 tttcggtttg gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcacagat
4681 atgaataaca gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca
4741 gagggatata tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc
4801 gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc ttcgacgact
4861 gagtgggctg ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa
4921 aagaaagcac aacttgagct ttataatgaa gtggctcaag aatatgggca cgatattcat
4981 aatatcgacc attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa
5041 gagatttgcc ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt
5101 tttgatgatt cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt
5161 gtattaaaag gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc
5221 gtgggaacgc cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata
5281 tcaaatattt gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg
5341 aagctcttcc agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat
5401 tagctaagga gaaagaaatg aaatttggat tgttcttcct taacttcatc aattcaacaa
5461 ctgttcaaga acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga
5521 attttgaaca gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc
5581 ctctgactgt ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa
5641 atcacatcat tacaactcat catcctgtcg ccatagcgga ggaagcttgc ttattggatc
5701 agttaagtga agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc
5761 atttttttaa tcgcccggtt gaatatcaac agcaactatt tgaagagtgt tatgaaatca
5821 ttaacgatgc tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta
5881 aaatatctgt aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa
5941 ccagtcatca tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg
6001 atgattctaa tgatgttaga tatgaatatg ctgaaagata taaagccgtt gcggataaat
6061 atgacgttga cctatcagag atagaccatc agttaatgat attagttaac tataacgaag
6121 atagtaataa agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc
6181 accctaatga aaatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa
6241 attatacgga gtgtataact gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg
6301 tattgctgtc ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg
6361 ttgatgataa tattaagaag taccacatga aatataccta ataggtacca ggaggatggc
6421 aaatatgact aaaaaaattt cattcattat taacggccag gttgaaatct ttcccgaaag
6481 tgatgattta gtgcaatcca ttaattttgg tgataatagt gtttacctgc caatattgaa
6541 tgactctcat gtaaaaaaca ttattgattg taatggaaat aacgaattac ggttgcataa
6601 cattgtcaat tttctctata cggtagggca aagatggaaa aatgaagaat actcaagacg
6661 caggacatac attcgtgact taaaaaaata tatgggatat tcagaagaaa tggctaagct
6721 agaggccaat tggatatcta tgattttatg ttctaaaggc ggcctttatg atgttgtaga
6781 aaatgaactt ggttctcgcc atatcatgga tgaatggcta cctcaggatg aaagttatgt
6841 tcgggctttt ccgaaaggta aatctgtaca tctgttggca ggtaatgttc cattatctgg
6901 gatcatgtct atattacgcg caattttaac taagaatcag tgtattataa aaacatcgtc
6961 aaccgatcct tttaccgcta atgcattagc gttaagtttt attgatgtag accctaatca
7021 tccgataacg cgctctttat ctgttatata ttggccccac caaggtgata catcactcgc
7081 aaaagaaatt atgcgacatg cggatgttat tgtcgcttgg ggagggccag atgcgattaa
7141 ttgggcggta gagcatgcgc catcttatgc tgatgtgatt aaatttggtt ctaaaaagag
7201 tctttgcatt atcgataatc ctgttgattt gacgtccgca gcgacaggtg cggctcatga
7261 tgtttgtttt tacgatcagc gagcttgttt ttctgcccaa aacatatatt acatgggaaa
7321 tcattatgag gaatttaagt tagcgttgat agaaaaactt aatctatatg cgcatatatt
7381 accgaatgcc aaaaaagatt ttgatgaaaa ggcggcctat tctttagttc aaaaagaaag
7441 cttgtttgct ggattaaaag tagaggtgga tattcatcaa cgttggatga ttattgagtc
7501 aaatgcaggt gtggaattta atcaaccact tggcagatgt gtgtaccttc atcacgtcga
7561 taatattgag caaatattgc cttatgttca aaaaaataag acgcaaacca tatctatttt
7621 tccttgggag tcatcattta aatatcgaga tgcgttagca ttaaaaggtg cggaaaggat
7681 tgtagaagca ggaatgaata acatatttcg agttggtgga tctcatgacg gaatgagacc
7741 gttgcaacga ttagtgacat atatttctca tgaaaggcca tctaactata cggctaagga
7801 tgttgcggtt gaaatagaac agactcgatt cctggaagaa gataagttcc ttgtatttgt
7861 cccataatag gtaaaagtat ggaaaatgaa tcaaaatata aaaccatcga ccacgttatt
7921 tgtgttgaag gaaataaaaa aattcatgtt tgggaaacgc tgccagaaga aaacagccca
7981 aagagaaaga atgccattat tattgcgtct ggttttgccc gcaggatgga tcattttgct
8041 ggtctggcgg aatatttatc gcggaatgga tttcatgtga tccgctatga ttcgcttcac
8101 cacgttggat tgagttcagg gacaattgat gaatttacaa tgtctatagg aaagcagagc
8161 ttgttagcag tggttgattg gttaactaca cgaaaaataa ataacttcgg tatgttggct
8221 tcaagcttat ctgcgcggat agcttatgca agcctatctg aaatcaatgc ttcgttttta
8281 atcaccgcag tcggtgttgt taacttaaga tattctcttg aaagagcttt agggtttgat
8341 tatctcagtc tacccattaa tgaattgccg gataatctag attttgaagg ccataaattg
8401 ggtgctgaag tctttgcgag agattgtctt gattttggtt gggaagattt agcttctaca
8461 attaataaca tgatgtatct tgatataccg tttattgctt ttactgcaaa taacgataat
8521 tgggtcaagc aagatgaagt tatcacattg ttatcaaata ttcgtagtaa tcgatgcaag
8581 atatattctt tgttaggaag ttcgcatgac ttgagtgaaa atttagtggt cctgcgcaat
8641 ttttatcaat cggttacgaa agccgctatc gcgatggata atgatcatct ggatattgat
8701 gttgatatta ctgaaccgtc atttgaacat ttaactattg cgacagtcaa tgaacgccga
8761 atgagaattg agattgaaaa tcaagcaatt tctctgtctt aaaggatcct gaggaggaaa
8821 aacaggtatg acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga
8881 tttgattttt tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa
8941 gaaacttgtg cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta
9001 ctgtcaggca cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc
9061 aacatcggtt tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg
9121 gtttaccagt agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat
9181 tgagagactc ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca
9241 tcaaatagaa ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa
9301 atatgttatg agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg
9361 aatagatttt gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct
9421 ttgtcttatt ggttcgccat actttattta tttactctgc cattatatga aagataaaaa
9481 aatctcattt tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta
9541 cgaaaaagaa tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct
9601 cagtgatatt agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt
9661 tgaggatgaa atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc
9721 tgaaacgttg aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc
9781 agcaaccagt tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata
9841 tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa
9901 agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga tatcctgcaa tcaagcttat
9961 cgatgtcgac gtagttaact agcgtacgat cgactgccag gcatcaaata aaacgaaagg
10021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtccggccat catggccgcg
10081 gtgatca
//
