SETD2-GFP

LOCUS       SETD2-GFP               11030 bp ds-DNA     circular SYN 05-JAN-2023
DEFINITION  mammalian expression of SETD2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    SETD2-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Carvalho S, Vitor AC, Sridhara SC, Martins FB, Raposo AC, Desterro 
            JM, Ferreira J, de Almeida SF
  TITLE     SETD2 is required for DNA double-strand break repair and activation 
            of the p53-mediated checkpoint.
  JOURNAL   Elife. 2014 May 6;3:e02482. doi: 10.7554/eLife.02482.
  PUBMED    24843002
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 5, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        21..324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        325..528
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     479..499
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      567..576
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             573..1289
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             573..1289
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(618..639)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(879..898)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1226..1247
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7111..7122
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    7778..7899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7815..7834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7869..7888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7906..8361)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8043..8064)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8255..8274
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8388..8492
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8494..8851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8517..8537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8702..8837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8764..8783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8886..9680
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8940..8959)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9550..9569
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9868..9887)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9912..9959
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10777..10796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
      541 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca
      601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1261 tcactctcgg catggacgag ctgtacaagt ccggactcag atctatggaa agaagaggca
     1321 agtattcttc aaaactagaa agagaatcta aaaggacttc agaaaatgaa gcaattaaaa
     1381 gatgttgttc tccccctaat gaactgggat tccgacgagg gtcatcatat tctaagcatg
     1441 acagtagtgc ttcccgttat aaatctaccc tttcaaaacc tatacccaag tctgataaat
     1501 ttaaaaattc tttctgttgt acagaattaa atgaagaaat caaacagtct cattctttta
     1561 gtttacagac accttgttca aaaggtagtg aattaagaat gattaataaa aatcctgaaa
     1621 gagaaaaggc tgggtctcca gctccatcaa atcgattaaa tgattcacct actttaaaaa
     1681 agctagatga attgcctatt tttaagtccg aatttataac acatgatagc catgatagta
     1741 ttaaggaatt agactcttta tctaaagtga agaatgatca attaagaagt ttttgtccca
     1801 tagaattaaa tataaatgga tctcctgggg cagaatctga tttggcaaca ttttgcactt
     1861 ctaaaactga tgctgtttta atgacttctg atgatagtgt gactggatcg gaattatccc
     1921 ctttggtcaa agcatgcatg ctttcatcaa atggatttca gaatattagt aggtgcaaag
     1981 aaaaagactt ggatgatacc tgcatgctgc ataagaagtc agaaagccca tttagagaaa
     2041 cagaacctct ggtgtcacca caccaagata aactcatgtc tatgccagtt atgactgtgg
     2101 attattccaa aacagtagtt aaagaaccag ttgatacgag ggtttcttgc tgcaaaacca
     2161 aagattcaga catatactgt actttgaacg atagcaaccc ttctttgtgt aactctgaag
     2221 ctgaaaatat tgagccttca gttatgaaga tttcttcaaa tagctttatg aatgtgcatt
     2281 tggaatcaaa accagttata tgtgatagta gaaatttgac agatcactca aaatttgcat
     2341 gtgaagaata taagcagagc atcggtagca ctagttcagc ttctgttaat cattttgatg
     2401 atttatatca acctattggg agttcaggta ttgcttcatc tcttcagagt cttccaccag
     2461 gaataaaggt ggacagtcta actctcttga aatgcggaga gaacacatct ccagttctgg
     2521 atgcagtgct aaagagtaaa aaaagttcag agtttttaaa gcatgcaggg aaagaaacaa
     2581 tagtagaagt aggtagtgac cttcctgatt caggaaaggg atttgcttcc agggagaaca
     2641 ggcgtaataa tgggttatct gggaaatgtt tgcaagaggc tcaagaagaa gggaattcca
     2701 tattgcctga aagaagagga agaccagaaa tctctttaga tgaaagagga gaaggaggac
     2761 atgtgcatac ttctgatgac tcagaagttg tattttcttc ttgtgatttg aatttaacca
     2821 tggaagacag tgatggtgta acttatgcat taaagtgtga cagtagtggt catgccccag
     2881 aaattgtgtc tacagttcat gaagattatt ctggctcttc tgaaagttca aatgatgaaa
     2941 gtgattcaga agatacagat tcggatgata gcagtattcc aagaaaccgt ctccagtctg
     3001 ttgtggttgt gccaaagaat tctactttgc ccatggaaga aacaagtcct tgttcttctc
     3061 ggagcagtca aagttataga cactattctg accattggga agatgagaga ttggagtcaa
     3121 ggagacattt gtatgaggaa aaatttgaaa gtatagcaag taaagcctgt cctcaaactg
     3181 ataagttttt ccttcataaa ggaacagaga agaatccgga aatttctttt acacagtcca
     3241 gtagaaaaca aatagataac cgcctgcctg aactttctca tcctcagagt gatggggttg
     3301 atagtacaag tcatacagat gtgaaatctg accctctggg tcacccaaat tcagaggaaa
     3361 ccgtgaaagc caaaatacct tctaggcagc aagaagagct gccaatttat tcttctgatt
     3421 ttgaagatgt cccaaataag tcttggcaac agaccacttt ccaaaacagg ccagatagta
     3481 gactgggaaa aacagaattg agtttttctt cctcttgtga gataccacat gtggatggct
     3541 tgcactcatc agaagagctc agaaacttag gttgggactt ctctcaagaa aagccttcta
     3601 ccacgtatca gcaacctgac agtagctatg gagcttgtgg tggacacaag tatcagcaaa
     3661 atgcagaaca gtatggtggg acacgtgatt actggcaagg caatggttac tgggatccaa
     3721 gatcaggtag acctcctgga actggggttg tgtatgatcg aactcaagga caagtaccag
     3781 attccctaac agatgatcgt gaagaagagg agaattggga tcaacaggat ggatcccatt
     3841 tttcagacca gtccgataaa tttcttctat cccttcagaa agacaagggg tcagtgcaag
     3901 cacctgaaat aagcagcaat tccattaagg acactttagc tgtgaatgaa aagaaagatt
     3961 tttcaaaaaa cttagaaaaa aatgatatca aagatagagg gcctcttaaa aaaaggaggc
     4021 aggaaataga gagtgattct gaaagtgatg gtgagcttca ggacagaaag aaagttagag
     4081 tggaggtaga gcagggagag acatcagtgc ccccaggttc agcactggtt gggccctcct
     4141 gtgtcatgga tgacttcagg gacccacagc gatggaagga atgtgccaag caagggaaaa
     4201 tgccatgtta ctttgatctt attgaagaaa atgtttattt aacagaaaga aagaagaata
     4261 aatctcatcg agatattaag cgaatgcagt gtgagtgtac acctctttct aaagatgaaa
     4321 gagctcaagg tgaaatagca tgtggggaag attgtcttaa tcgtcttctc atgattgaat
     4381 gttcttctcg gtgtccaaat ggggattatt gttccaatag acggtttcag agaaaacagc
     4441 atgcagatgt ggaagtcata ctcacagaaa agaaaggctg gggcttgaga gctgccaaag
     4501 accttccttc gaacaccttt gtcctagaat attgtggaga ggtactcgat cataaagagt
     4561 ttaaagctcg agtgaaggag tatgcacgaa acaaaaacat ccattactat ttcatggccc
     4621 tgaagaatga tgagataata gatgccactc aaaaaggaaa ttgctctcgt ttcatgaatc
     4681 acagctgtga accaaattgt gaaacccaaa aatggactgt gaacggacaa ctgagggttg
     4741 ggttttttac caccaaactg gttccttcag gctcagagtt aacgtttgac tatcagttcc
     4801 agagatatgg aaaagaagcc cagaaatgtt tctgcggatc agccaattgc cggggttacc
     4861 tgggaggaga aaacagagtc agcatcagag cagcaggagg gaaaatgaag aaggaacgat
     4921 ctcgtaagaa ggattcagtg gatggagagc tagaagctct gatggaaaat ggtgagggtc
     4981 tctctgataa aaaccaggtg ctcagcttat cccggctaat ggttagaatt gaaactttgg
     5041 agcagaaact tacctgtctg gaactcatac agaacacaca ctcacagtcc tgcctgaagt
     5101 cctttctgga acgtcatggg ctgtctttgt tgtggatctg gatggcagag ctaggtgacg
     5161 gccgggaaag taaccagaag cttcaggaag agattataaa gactttggaa cacttgccca
     5221 ttcctactaa aaatatgttg gaggaaagca aagtacttcc aattattcaa cgctggtctc
     5281 agactaagac tgctgtccct ccgttgagtg aaggagatgg gtattctagt gagaatacat
     5341 cgcgtgctca tacaccactc aacacacctg atccttccac caagctgagc acagaagctg
     5401 acacagacac tcccaagaaa ctaatgtttc gcagactgaa aattataagt gaaaatagca
     5461 tggacagtgc aatctctgat gcaaccagtg agctagaagg caaggatggc aaagaggatc
     5521 ttgatcaatt agaaaatgtc cctgtagagg aagaggaaga attgcagtca caacagctac
     5581 tcccacaaca gctgcctgaa tgcaaagttg atagtgaaac caacatagaa gctagtaagc
     5641 tacctacatc tgaaccagaa gctgacgctg aaatagagct caaagagagc aacggcacaa
     5701 aactagaaga acctattaat gaagaaacac catcccaaga tgaagaggag ggtgtgtctg
     5761 atgtggagag tgaaaggagc caagaacagc cagataaaac agtggatata agtgatttgg
     5821 ccaccaaact cctggacagt tggaaagacc taaaggaggt atatcgaatt ccaaagaaaa
     5881 gtcaaactga aaaggaaaac acaacaactg aacgaggaag ggatgctgtt ggcttcagag
     5941 atcaaacacc tgccccgaag actcctaata ggtcaagaga gagagaccca gacaagcaaa
     6001 ctcaaaataa agagaaaagg aaacgaagaa gctccctctc accaccctct tctgcctatg
     6061 agcggggaac aaaaaggcca gatgacagat atgatacacc aacttctaaa aagaaagtac
     6121 gaattaaaga ccgcaataaa ctttctacag aggaacgccg gaagttgttt gagcaagagg
     6181 tggctcaacg ggaggctcag aaacaacagc aacagatgca gaacctggga atgacatcac
     6241 cactgcccta tgactctctt ggttataatg ccccgcatca tccctttgct ggttacccac
     6301 caggttatcc catgcaggcc tatgtggatc ccagcaaccc taatgctgga aaggtgctcc
     6361 tgcccacacc cagcatggac ccagtgtgtt ctcctgctcc ttatgatcat gctcagccct
     6421 tggtgggaca ttctacagaa cccctttctg cccctccacc agtaccagtg gtgccacatg
     6481 tggcagctcc tgtggaagtt tccagttccc agtatgtggc ccagagtgat ggtgtagtac
     6541 accaagactc cagcgttgct gtcttgccag tgccggcccc cggcccagtt cagggacaga
     6601 attatagtgt ttgggattca aaccaacagt ctgtcagtgt acagcagcag tactctcctg
     6661 cacagtctca agcaaccata tattatcaag gacagacatg tccaacagtc tatggtgtga
     6721 catcacctta ttcacagaca actccaccaa ttgtacagag ttatgcccag ccaagtcttc
     6781 agtatatcca ggggcaacag attttcacag ctcatccaca aggagtggtg gtacagccag
     6841 ccgcagcagt gactacaata gttgcaccag ggcagcctca gcccttgcag ccatctgaaa
     6901 tggttgtgac aaataatctc ttggatctgc cgcccccctc tcctcccaaa ccaaaaacca
     6961 ttgtcttacc tcccaactgg aagacagctc gagatccaga agggaagatt tattactacc
     7021 atgtgatcac aaggcagact cagtgggatc ctcctacttg ggaaagccca ggagatgatg
     7081 ccagccttga gcatgaagct gagatggacc tgggaactcc aacatatgat gaaaacccca
     7141 tgaaggcctc gaaaaagccc aagacagcag aagcagacac ctccagtgaa ctagcaaaga
     7201 aaagcaaaga agtattcaga aaagagatgt cccagttcat cgtccagtgc ctgaaccctt
     7261 accggaaacc tgactgcaaa gtgggaagaa ttaccacaac tgaagacttt aaacatctgg
     7321 ctcgcaagct gactcacggt gttatgaata aggagctgaa gtactgtaag aatcctgagg
     7381 acctggagtg caatgagaat gtgaaacaca aaaccaagga gtacattaag aagtacatgc
     7441 agaagtttgg ggctgtttac aaacccaaag aggacactga attagagtga ctgttgggcc
     7501 agggtgggag gatgggtggt caggtaagac agactctagg gagaggaaat cctgtgggcc
     7561 tttctgtccc acccctgtca gcactgtgct actgatgata catcaccctg gggaattcaa
     7621 ccctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     7681 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     7741 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     7801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     7921 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     7981 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     8041 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     8101 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     8161 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     8221 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     8281 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     8341 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8461 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     8521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8761 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8821 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     8881 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     8941 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     9001 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     9061 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     9121 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     9181 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     9241 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     9301 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     9361 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     9421 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     9481 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     9541 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     9601 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     9661 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     9721 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     9781 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     9841 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     9901 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     9961 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
    10021 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
    10081 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
    10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10981 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
//