| 质粒名称: | pBAD-6xHis-miRFP720-HO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000096 |
| 产品用途: | Expresses miRFP720 with Heme Oxygenase 1 (HO1) in bacteria for purification |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP720, HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pBAD-6xHis-miRFP720-HO1载体质粒基本信息
pBAD-6xHis-miRFP720-HO1质粒图谱载体图谱和pBAD-6xHis-miRFP720-HO1载体序列质粒序列多克隆位点信息
pBAD-6xHis-miRFP720-HO1质粒载体简介
pBAD-6xHis-miRFP720-HO1质粒序列载体序列
LOCUS pBAD-6xHis-miRFP720-HO1 5703 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5703)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5703
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
CDS 319..327
/codon_start=1
/label=Start
/translation="MGS"
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind 334..374
/label=0408-PB171-F
CDS 355..1302
/codon_start=1
/product="near-infrared fluorescent protein with an
emission peak at 720 nm, engineered from a bacterial
phytochrome (Shcherbakova and Verkhusha, 2013)"
/label=miRFP720
/note="derived from Rhodopseudomonas palustris RpBphP2"
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
GFDRVMIYRFASDFSGSVIAEDRCAEVESKLGLHYPASFIPAQARRLYTINPVRIIPDI
NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPNHLEFMRNIGMHGTMSISILRGERLWGL
IVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE"
regulatory 355..358
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1281..1329)
/label=0408-PB171-R
misc_feature 1306..2058
/label=to be cloned into PM283
CDS 1336..2058
/codon_start=1
/label=HO1
/translation="MSVNLASQLREGTKKSHSMAENVGFVKCFLKGVVEKNSYRKLVGN
LYFVYSAMEEEMAKFKDHPILSHIYFPELNRKQSLEQDLQFYYGSNWRQEVKISAAGQA
YVDRVRQVAATAPELLVAHSYTRYLGDLSGGQILKKIAQNAMNLHDGGTAFYEFADIDD
EKAFKNTYRQAMNDLPIDQATAERIVDEANDAFAMNMKMFNELEGNLIKAIGIMVFNSL
TRRRSQGSTEVGLATSEG"
primer_bind 1610..1634
/label=HO1-F
primer_bind complement(1769..1790)
/label=HO1-R
terminator 2274..2360
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 2452..2479
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 2498..2589
/gene="bla"
/label=AmpR promoter
CDS 2590..3450
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3621..4209
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4395..4535
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(4799..5677)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggcg
361 gaaggatccg tcgccaggca gcctgacctc ttgacctgcg acgatgagcc gatccatatc
421 cccggtgcca tccaaccgca tggactgctg ctcgccctcg ccgccgacat gacgatcgtt
481 gccggcagcg acaaccttcc cgaactcacc ggactggcga tcggcgccct gatcggccgc
541 tctgcggccg atgtcttcga ctcggagacg cacaaccgtc tgacgatcgc cttggccgag
601 cccggggcgg ccgtcggagc accgatcact gtcggcttca cgatgcgaaa ggacgcaggc
661 ttcatcggct cctggcatcg ccatgatcag ctcatcttcc tcgagctcga gcctccccag
721 cgggacgtcg ccgagccgca ggcgttcttc cgccgcacca acagcgccat ccgccgcctg
781 caggccgccg aaaccttgga aagcgcctgc gccgccgcgg cgcaagaggt gcggaagatt
841 accggcttcg atcgggtgat gatctatcgc ttcgcctccg acttcagcgg gtccgtgatc
901 gcagaggatc ggtgcgccga ggtcgagtca aaactaggcc tgcactatcc tgcctcattc
961 atcccggcgc aggcccgtcg gctctatacc atcaacccgg tacggatcat tcccgatatc
1021 aattatcggc cggtgccggt caccccagac ctcaatccgg tcaccgggcg gccgattgat
1081 cttagcttcg ccatcctgcg cagcgtctcg cccaaccatc tggagttcat gcgcaacata
1141 ggcatgcacg gcacgatgtc gatctcgatt ttgcgcggcg agcgactgtg gggattgatc
1201 gtttgccatc accgaacgcc gtactacgtc gatctcgatg gccgccaagc ctgcaagaga
1261 gtcgccgaga gactggccac ccagatcggc gtgatggaag agtaatagct gcagaagaag
1321 gagatataca tacccatgag tgtcaactta gcttcccagt tgcgggaagg gacgaaaaaa
1381 tcccactcca tggcggagaa cgtcggcttt gtcaaatgct tcctcaaggg cgttgtcgag
1441 aaaaattcct accgtaagct ggttggcaat ctctactttg tctacagtgc catggaagag
1501 gaaatggcaa aatttaagga ccatcccatc ctcagccaca tttacttccc cgaactcaac
1561 cgcaaacaaa gcctagagca agacctgcaa ttctattacg gctccaactg gcggcaagaa
1621 gtgaaaattt ctgccgctgg ccaagcctat gtggaccgag tccggcaagt ggccgctacg
1681 gcccctgaat tgttggtggc ccattcctac acccgttacc tgggggatct ttccggcggt
1741 caaattctca agaaaattgc ccaaaatgcc atgaatctcc acgatggtgg cacagctttc
1801 tatgaatttg ccgacattga tgacgaaaag gcttttaaaa atacctaccg tcaagctatg
1861 aatgatctgc ccattgacca agccaccgcc gaacggattg tggatgaagc caatgacgcc
1921 tttgccatga acatgaaaat gttcaacgaa cttgaaggca acctgatcaa ggcgatcggc
1981 attatggtgt tcaacagcct cacccgtcgc cgcagtcaag gcagcaccga agttggcctc
2041 gccacctccg aaggctaaga attcgaagct tggctgtttt ggcggatgag agaagatttt
2101 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
2161 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
2221 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
2281 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
2341 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
2401 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
2461 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
2521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
2581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
2641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
2701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
2761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
2821 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
2881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
2941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
3001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
3061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
3121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
3181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
3241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
3301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
3361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
3421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
3481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
3541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
3601 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
3661 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
3721 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
3781 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
3841 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
3901 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
3961 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
4021 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
4081 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
4141 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
4201 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
4261 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
4321 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
4381 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
4441 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
4501 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
4561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
4621 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
4681 tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag
4741 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
4801 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
4861 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
4921 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
4981 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
5041 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
5101 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
5161 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
5221 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
5281 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
5341 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
5401 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
5461 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
5521 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
5581 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
5641 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
5701 gag
//
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