| 质粒名称: | pAAV-CAG-mBlueberry2-P2A-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000195 |
| 产品用途: | AAV, Expresses mBlueberry2 and GFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳细胞表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mBlueberry2, GFP |
| 物种来源: | Synthetic, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pAAV-CAG-mBlueberry2-P2A-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-mBlueberry2-P2A-GFP载体质粒基本信息
pAAV-CAG-mBlueberry2-P2A-GFP质粒图谱载体图谱和pAAV-CAG-mBlueberry2-P2A-GFP载体序列质粒序列多克隆位点信息
pAAV-CAG-mBlueberry2-P2A-GFP质粒载体简介
pAAV-CAG-mBlueberry2-P2A-GFP质粒序列载体序列
LOCUS pAAV-CAG-mBlueberry2-P2A-GFP 6241 bp DNA circular SYN 29-MAY-2023
DEFINITION Expresses TagRFP658 and GFP in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6241)
TITLE Rapid Directed Molecular Evolution of Fluorescent Proteins in
Mammalian Cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6241)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..6241
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
CDS 983..1690
/codon_start=1
/label=mBlueberry2
/translation="MVSKGEENNVAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFLFGSKVYIKHPADIPDYFKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEAFSERMYPEDGALK
SEIKTRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDIVSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
regulatory 983..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1715..1771
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1784..2500
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGRLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2525..3113
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2996..3007)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3156..3277
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 3439..3568
/label=AAV2 ITR
rep_origin complement(3805..4393)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4564..5424)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5425..5529)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5555..6010)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 6082..6211
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgag caagggcgag gagaacaacg tggccatcat
1021 caaggagttc atgagattca aggtgcacat ggagggcagc gtgaacggcc acgagttcga
1081 gatcgagggc gagggcgagg gcagacccta cgagggcacc cagaccgcca agctgaaggt
1141 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg agcccccagt tcctgttcgg
1201 cagcaaggtg tacatcaagc accccgccga catccccgac tacttcaagc tgagcttccc
1261 cgagggcttc aagtgggaga gagtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
1321 ccaggacagc agcctgcagg acggcgtgtt catctacaag gtgaagctga gaggcaccaa
1381 cttccccagc gacggccccg tgatgcagaa gaagaccatg ggctgggagg ccttcagcga
1441 gagaatgtac cccgaggacg gcgccctgaa gagcgagatc aagaccagac tgaagctgaa
1501 ggacggcggc cactacgacg ccgaggtgaa gaccacctac aaggccaaga agcccgtgca
1561 gctgcccggc gcctacaacg tgaacatcaa gctggacatc gtgagccaca acgaggacta
1621 caccatcgtg gagcagtacg agagagccga gggcagacac agcaccggcg gcatggacga
1681 gctgtacaag cgaccggttc acgtgggaag tggggctaca aacttctcac tgctgaaaca
1741 ggccggggat gtggaggaaa acccaggacc acacgtgact agtgtgagca agggcgagga
1801 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
1861 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
1921 catctgcacc accggcaggc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
1981 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
2041 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
2101 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
2161 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
2221 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
2281 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
2341 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
2401 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
2461 cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcgata tcaagcttat
2521 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
2581 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
2641 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
2701 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
2761 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
2821 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
2881 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
2941 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
3001 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
3061 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
3121 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
3181 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
3241 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
3301 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
3361 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
3421 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
3481 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
3541 cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag
3601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
3661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
3781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
3841 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
3901 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
3961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
4021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
4081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
4141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
4201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
4261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
4321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
4381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
4501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
4561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
4621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
4681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
4741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
4801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
4861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
4921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
4981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
5041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
5101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
5161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
5221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
5281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
5341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
5401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
5461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5521 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
5581 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
5641 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
5701 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
5761 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
5821 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
5881 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
5941 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
6001 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
6061 gcctcttcgc tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
6121 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
6181 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
6241 t
//
