pAAVLINKv1-pCALM1-Cre-3'-CDC42BPB-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-CDC42BPB-FLAG              6229 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..2942
                     /codon_start=1
                     /gene="CDC42BPB"
                     /gene_synonym="CHOCNS; MRCKB"
                     /product="serine/threonine-protein kinase MRCK betaisoform 
                     1"
                     /EC_number="2.7.11.1"
                     /label=CDC42BPB
                     /note="isoform 1 is encoded by transcript variant 1; 
                     serine/threonine-protein kinase MRCK beta; myotonic 
                     dystrophy protein kinase-like beta; DMPK-like beta; 
                     myotonic dystrophy kinase-related CDC42-binding kinase 
                     beta; CDC42BP-beta; CDC42 binding protein kinase beta 
                     (DMPK-like)"
                     /db_xref="GI:115527097"
                     /db_xref="CCDS:CCDS9978.1"
                     /db_xref="GeneID:9578"
                     /db_xref="HGNC:HGNC:1738"
                     /db_xref="MIM:614062"
                     /protein_id="NP_006026.3"
                     /translation="VVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTR
                     DVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGC
                     QLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLC
                     VGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGL
                     YVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRP
                     LNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEE
                     RLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPA
                     PTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNS
                     SNPSGPPSPNSPHRSQLPLEGLEQPACDT"
     misc_feature    1691..1696
                     /label=SDS
     misc_feature    2348..2353
                     /label=SDS
     misc_feature    2656..2661
                     /label=SDS
     CDS             2949..3014
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3018..3449
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3162..3167
                     /label=SDS
     repeat_region   3492..3632
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3492..3621
                     /label=AAV2 ITR
     repeat_region   3493..3633
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3707..4162
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4444..4548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4549..5409
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5580..6168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtcgtgcat gttcccttgg
     1501 aagcctacga cagctcgctg cctctcatca aggccatcct gacagctgcc atcgtggatg
     1561 cagacaggat tgcagtcggc ctagaagaag ggctctatgt catagaggtc acccgagatg
     1621 tgatcgtccg tgccgctgac tgtaagaagg tacaccagat cgagcttgct cccagggaga
     1681 agatcgtaat cctcctctgt ggccggaacc accatgtgca cctctatccg tggtcgtccc
     1741 ttgatggagc ggaaggcagc tttgacatca agcttccgga aaccaaaggc tgccagctca
     1801 tggccacggc cacactcaag aggaactctg gcacctgcct gtttgtggcc gtgaaacggc
     1861 tgatcctttg ctatgagatc cagagaacga agccattcca cagaaagttc aatgagattg
     1921 tggctcccgg cagcgtgcag tgcctggcgg tgctcaggga caggctctgt gtgggctacc
     1981 cttctgggtt ctgcctgctg agcatccagg gggacgggca gcctctaaac ctggtaaatc
     2041 ccaatgaccc ctcgcttgcg ttcctctcac aacagtcttt tgatgccctt tgtgctgtgg
     2101 agctcgaaag cgaggagtac ctgctttgct tcagccacat gggactgtac gtggacccgc
     2161 aaggccggag ggcacgcgcg caggagctca tgtggcctgc ggctcctgtc gcctgtagtt
     2221 gcagccccac ccacgtcacg gtgtacagcg agtatggcgt ggacgtcttt gatgtgcgca
     2281 ccatggagtg ggtgcagacc atcggcctgc ggaggataag gcccctgaac tctgaaggca
     2341 ccctcaacct cctcaactgc gagcctccac gcttgatcta cttcaagagc aagttctcgg
     2401 gagcggttct caacgtgccg gacacctccg acaacagcaa gaagcagatg ctgcgcacca
     2461 ggagcaaaag gcggttcgtc ttcaaggtcc cagaggaaga gagactgcag cagaggcgag
     2521 agatgcttag agacccagaa ttgagatcca aaatgatatc caacccaacc aacttcaacc
     2581 acgtggccca catgggccca ggcgacggca tgcaggtgct catggacctg cctctgagtg
     2641 ctgtgccccc ctcccaggag gaaaggccgg gccccgctcc caccaacctg gctcgccagc
     2701 ctccatccag gaacaagccc tacatctcgt ggccctcatc aggtggatcg gagcctagcg
     2761 tgactgtgcc tctgagaagt atgtctgatc cagaccagga ctttgacaaa gagcctgatt
     2821 cggactccac caaacactca actccatcga atagctccaa ccccagcggc ccaccgagcc
     2881 ccaactcccc ccacaggagc cagctccccc tcgaaggcct ggagcagccg gcctgtgaca
     2941 cctgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
     3001 atgacgatga caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt
     3061 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3181 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3241 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
     3301 tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     3361 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     3421 ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac
     3481 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3541 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3601 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3661 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3781 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3841 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3901 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3961 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4021 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
     4081 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4141 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     4201 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4261 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4321 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4381 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4441 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5461 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5521 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5581 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5641 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5701 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5761 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5821 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5881 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5941 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6001 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6061 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6121 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6181 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//