| 质粒名称: | pBFC0625 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 186691 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | R6K conditional origin, bla, RP4 oriT |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 12613 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | - |
| 载体抗性: | 氨苄青霉素和庆大霉素，100和10μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | EC100D pir+ |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | tnsA tnsB tnsC tnsD tniQ cas7 cas6 Vc_lacZ_α_1 Spacer crRNA Tn7 transposon GmR+sfGFP VcDART Cargo |
| 物种来源: | Vibrio cholerae |
|
文献引用方法 |
|
| 材料和方法部分: | pBFC0625 was a gift from Jennifer Doudna (Addgene plasmid # 186691 ; http://n2t.net/addgene:186691 ; RRID:Addgene_186691) |
| 参考文献部分: | Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. 10.1038/s41564-021-01014-7 PubMed 34873292 |
买家导航
pBFC0625载体质粒基本信息
pBFC0625质粒图谱载体图谱和pBFC0625载体序列质粒序列多克隆位点信息
pBFC0625质粒载体简介
pBFC0625质粒序列载体序列
LOCUS pBFC0625 12613 bp ds-DNA circular SYN 09-SEP-2022
DEFINITION VcDART under control of Lac promoter, Vc_lacZ_alpha_1 gRNA,
GmR+sfGFP cargo.
ACCESSION .
VERSION .
KEYWORDS pBFC0625
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12613)
AUTHORS Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL,
Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang
K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM,
Banfield JF, Doudna JA
TITLE Species- and site-specific genome editing in complex bacterial
communities.
JOURNAL Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7.
Epub 2021 Dec 6.
PUBMED 34873292
REFERENCE 2 (bases 1 to 12613)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 9, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12613
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 270..341
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 357..384
/label=T7Te terminator
/note="phage T7 early transcription terminator"
primer_bind 395..414
/label=T7
/note="T7 promoter, forward primer"
promoter 395..413
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
oriT 557..665
/note="incP origin of transfer"
promoter 858..962
/gene="bla"
/label=AmpR promoter
CDS 963..1823
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1181..1200)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1859..2247
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
promoter 2397..2427
/label=lac promoter
/note="promoter for the E. coli lac operon"
RBS 2428..2439
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 4524..4535
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 11031..11059
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
CDS 11248..11781
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/label=GmR
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
RBS 12140..12151
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 12158..258
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
ORIGIN
1 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
61 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
121 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac
181 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
241 atggatgaac tgtacaaatg accaggcatc aaataaaacg aaaggctcag tcgaaagact
301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
361 caccttcggg tgggcctttc tgcgtttata gctgtaatac gactcactat aggggataag
421 agacggctca acgcccgtct cacagcgcaa tatcaattta tgggtgtgat aattatcaat
481 ttatgggtgt aattatcatt ttatggttgt atcaacaagg aggactttgg caggtgcctg
541 caggattctt gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
601 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
661 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
721 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
781 agggttatgc agcggaaaag aatgtcatga taataatggt ttcttagacg tcaggtggca
841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
1021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
1081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
1141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
1201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
1261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
1321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
1381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
1441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
1501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
1561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
1621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct
1681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
1741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
1801 cctcactgat taagcattgg taactgtcag acccggccac gatgcgtccg gcgtagagga
1861 tctgaagatc agcagttcaa cctgttgata gtacgtacta agctctcatg tttcacgtac
1921 taagctctca tgtttaacgt actaagctct catgtttaac gaactaaacc ctcatggcta
1981 acgtactaag ctctcatggc taacgtacta agctctcatg tttcacgtac taagctctca
2041 tgtttgaaca ataaaattaa tataaatcag caacttaaat agcctctaag gttttaagtt
2101 ttataagaaa aaaaagaata tataaggctt ttaaagcttt taaggtttaa cggttgtgga
2161 caacaagcca gggatgtaac gcactgagaa gcccttagag cctctcaaag caattttgag
2221 tgacacagga acacttaacg gctgacatgg gcaagtttac tcatatatac tttagattga
2281 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2341 gaccaaaatc ccttaacgtg agttttcgtt ccactgacac ctgcccgaac catatcttta
2401 cactttatgc ttccggctcg tatgttgaaa gaggagaaag gatcaatggc gacaagttta
2461 cctacgccct cagcaattac gacttcggcg ttagagtatg cattccatac tcccgctcgc
2521 aatctaacga aatctcgcgg aaaaaacatt catcgttatg tcagtgtaaa gatgagtaag
2581 aggattacgg tagaatctac tctagagtgt gatgcctgct atcactttga ttttgagcca
2641 agtattgttc gcttttgcgc tcaaccgatt cgatttttat attatctcaa tggtcagtct
2701 cactcctatg ttcctgactt tctagttcaa tttgatacca acgagtttgt tctatatgaa
2761 gtaaagtcag cttatgctaa gaacaaacct gattttgatg ttgaatggga ggcgaaagta
2821 aaagcagcaa ctgaactagg gctagaattg gagcttgttg aagagagtga tattagggat
2881 acggttgtat taaataatct taagcgcatg catcgttatg cttcgaaaga tgagctgaat
2941 aacgtacata actctctctt aaaaataata aagtacaatg gcgcccaatc tgcaagatgc
3001 ttgggagaac agttgggttt aaaaggccga actgttttac caattttgtg cgatttgctg
3061 tcaaggtgtt tactcgatac acgtttggat aagcctctat ctcttgaatc tcgatttgag
3121 ttggccagtt atggctaaga aagggttctc aagtttccat agaaaagcag tctcttccca
3181 agatacgctt gaatcgatag agcttgtctc tagcgctaat tgtctagaaa gtgttacgta
3241 tcaagatata tcagcatttc ccgaaacaat tgcggtagag attaatttcc gattaagcat
3301 tcttcgcttt ttagcgcgaa agtgcgaaac cattgtggcc aaatcaattg aaccacatcg
3361 tgtagagcta cagcaaaact atagtagaaa aatacccagt gcaataacga tatatcgatg
3421 gtggcttgct tttcgaaaat cagactacaa ccccattagc ttagcaccta atatcaagga
3481 tagaggtaat agagagacaa aagtgtcaac agttgttgat tctattatgg aacaggcagt
3541 tgaaagagtt atatctggac gaaaagtcaa tgttagctct gcatataaac gtgttcgacg
3601 aaaagttcgt caatacaatc taactcatgg aacgaaatac acgtatccta agtacgaatc
3661 tgtaagaaag cgagtaaaaa agaaaacccc atttgagtta ttagccgcag ggaaagggga
3721 gagagtagct aagagagagt ttcgccgaat gggaaaaaag atcctcacgt ctagcgtgct
3781 agagagggtt gaaatagatc acactgtcgt tgaccttttt gcagtacacg aagagtatcg
3841 aatcccattg ggccgacctt ggcttactca attggttgat tgttacagta aagctgtaat
3901 cggtttttat ttaggtttcg agcctcctag ctatgtgtcg gtttcccttg cacttaagaa
3961 tgcaatacaa cgcaaagatg acttaatctc ctcgtatgaa tcgatcgaga atgaatggct
4021 atgttatggc atcccagacc tactcgtaac tgataatggt aaagagtttt tgtcgaaagc
4081 atttgatcaa gcatgtgaat cactattgat caatgtgcat caaaataaag ttgaaacgcc
4141 cgacaacaaa cctcatgttg aacgtaacta cgggactatt aatacttctc tgttagacga
4201 tttacctggg aaatccttca gccagtacct tcaaagagaa gggtacgact ctgtgggaga
4261 agctaccctt acactcaatg agattagaga aatttactta atttggttgg tggatattta
4321 tcataaaaaa cccaatcaga gaggcactaa ttgtcctaat gttgcgtgga aaaagggttg
4381 tcaagaatgg gaaccagagg agttctctgg ttctaaagac gaattagact ttaaatttgc
4441 tattgttgat tacaaacaac ttactaaagt agggataact gtctacaaag aactgagtta
4501 tagcaatgac cgtttagctg aatatagagg gaagaaagga aaccataaag ttcagttcaa
4561 gtataaccct gagtgtatgg cagttatttg ggtgttggat gaggatatga atgagtactt
4621 tacagttaat gcgattgact acgaatatgc aagtagagta tcactttggc aacataaata
4681 taacatgaaa tatcaagcag aactaaattc agcagaatat gatgaggaca aggaaattga
4741 tgcagaaata aaaattgaag aaatcgcaga tcgttcaatt gttaagacta acaaaatcag
4801 agctcggagg cgtggcgcta ggcatcaaga gaatagcgca agggctaagt caatcagtaa
4861 tgcgaacccg gcctcgatac aaaaacatga agatgaaatc gttagtgcag ataatgacga
4921 ttgggatatt gattatgtct gagaaatcgt caaatgagtg aaacgcgtga ggctcgtata
4981 tcaagagcta aaagggcatt tgtatccaca ccgagcgtta ggaaaatctt gagttacatg
5041 gatagatgta gagatctatc agacctagag tctgagccta catgcatgat ggtctatggt
5101 gcttcgggtg taggtaaaac gaccgtcatc aagaaatact taaatcaaaa cagaagagag
5161 tccgaagccg ggggcgatat aataccggtt ttgcatattg agttgccaga caatgcgaag
5221 ccagtagatg cagcaaggga attgctggtt gaaatgggtg acccgctagc actttatgaa
5281 actgacttag ctagattgac gaaaagactg actgaattaa tccctgcggt cggcgtgaag
5341 ctgattatta tcgatgagtt ccaacatttg gtggaagaaa ggtcaaatcg ggttcttacc
5401 caagtaggta attggctaaa aatgatactt aacaaaacga aatgtccaat tgttatattt
5461 ggtatgccat actcaaaagt tgtactgcaa gcaaactcgc aacttcacgg gcgattttcc
5521 attcaggttg aactgcgccc ctttagctac cagggaggta gaggtgtatt taaaactttt
5581 ttggaatacc ttgataaagc cctacctttt gaaaaacagg ctggcttagc caacgaaagt
5641 ttgcagaaaa aattgtatgc attctctcag ggaaacatgc gttcgttgag aaaccttatt
5701 tatcaagcat ctatcgaagc aattgataat cagcatgaaa cgataaccga agaagatttc
5761 gtttttgcat cgaagttgac atcgggcgat aaacccaact catggaaaaa tccttttgag
5821 gagggtgttg aggtaacaga agatatgtta cgaccgccac caaaagatat tggttgggag
5881 gactatttga gacattcaac cccgagagtg agtaaaccag gtagaaataa aaactttttc
5941 gaataaccta ggctgctgcc accgctgagc aataaccatc ttagtatatt agttaagtat
6001 aagaaggaga tatacatatg tttttgcaaa gacctaaacc ttacagcgat gaaagtttag
6061 aaagtttctt tatccgagtg gctaacaaaa atggctacgg tgatgtccat cgcttcctag
6121 aagccactaa acgattcctt caagacattg accataatgg ctatcaaacc tttccgactg
6181 atataactcg gataaaccca tactcagcta aaaacagttc cagcgcacga actgcgtcat
6241 tcctgaaact tgcacaattg acatttaatg aaccgccaga gctacttggg ttggcaatta
6301 acagaacaaa catgaaatac tcgccgtcaa ctagcgcggt tgttagaggt gcagaagtct
6361 ttcctcgcag tttactacgg acgcactcca tcccctgctg tcctttgtgt ctgcgagaaa
6421 atggctacgc ctcctacctt tggcactttc aggggtacga atactgccac agccataacg
6481 tacctttaat taccacttgt agctgtggta aggagtttga ctaccgagta tctgggttaa
6541 agggcatttg ctgcaaatgc aaggagccta tcaccttaac cagcagggag aacggtcatg
6601 aggcagcgtg tactgtttca aactggcttg ctggccatga atctaaacct ctgccaaatc
6661 ttcctaaaag ctaccgatgg ggtttagttc attggtggat gggtattaaa gatagcgagt
6721 tcgatcactt ttcgttcgtt caatttttct caaactggcc aaggtcattc cactcgataa
6781 tcgaagatga agtagagttc aaccttgagc atgctgttgt cagcacgtct gaattacgac
6841 taaaagatct tcttggtcga ttgtttttcg gttcaattcg gttacctgag cggaatcttc
6901 aacacaatat catccttggt gagcttctct gctatttaga aaatcgttta tggcaagaca
6961 agggattaat cgccaacctc aaaatgaacg cgttagaggc gactgtaatg ttaaattgta
7021 gcctcgatca gattgcatca atggttgaac aacgcatctt gaagccaaat cgaaaaagca
7081 agcccaacag ccctcttgat gttaccgatt atctatttca tttcggcgat attttctgtc
7141 tttggttagc tgagttccaa agcgatgagt ttaaccgttc gttttatgtg tcgaggtggt
7201 aaatgcaaac tctgaaagaa ctaatcgcat ccaatcctga cgacttaaca actgaactta
7261 agagagcatt tcgtccactc acaccgcata ttgcaattga tggtaatgaa cttgacgcac
7321 tgacgatatt agtcaattta accgataaga ctgatgatca gaaagacctg ctcgatcgag
7381 ccaaatgcaa gcaaaaactt cgagatgaaa aatggtgggc tagctgcata aattgcgtta
7441 attacagaca aagccataac ccaaaattcc cggatatacg ttctgaaggc gtgattcgaa
7501 cccaagccct gggtgaatta ccgagtttcc tactctcgtc ctcaaaaatc ccaccatacc
7561 attggtcata tagccatgat tcaaaatacg tcaacaaaag cgcattcctc accaatgagt
7621 tttgttggga tggtgagatc tcatgtttgg gtgagcttct taaagatgca gatcacccac
7681 tttggaatac tttgaaaaag ttaggttgtt ctcaaaaaac ttgcaaagca atggcaaaac
7741 aactagctga tattactctc acgactatca atgtcacact cgcaccaaat tacctgactc
7801 aaatatctct ccccgatagt gatacatcct acatttcact ctctcctgtt gcatcgctat
7861 cgatgcaaag ccactttcat cagaggctcc aagatgaaaa tcggcatagc gcgataacgc
7921 ggtttagccg aactaccaac atgggggtca ctgcgatgac atgtggcggc gcatttagaa
7981 tgttaaagtc tggcgctaag ttttccagcc cccctcatca ccgattaaac agtaaacgaa
8041 gttggttgac gtcagagcat gttcagtcat taaaacagta ccagcgcctc aacaaaagcc
8101 tcatacctga aaactctcgg attgcactcc gtagaaaata caaaatcgag cttcaaaata
8161 tggtcagatc ttggtttgca atgcaagacc atacccttga ttcgaacata cttatccaac
8221 atttgaatca tgacctatct tacttaggag ccacaaaacg ttttgcatac gatccagcga
8281 tgaccaagct ctttactgag cttttgaaac gagagttatc aaattcaatc aataatggtg
8341 agcaacacac taatggatcg tttttagtcc taccgaatat cagggtttgt ggcgcaacag
8401 ctttaagctc cccggtaacg gtggggattc catcacttac agctttcttt ggcttcgttc
8461 acgcatttga acggaatata aatcgcacca cctcatcgtt tcgtgttgaa tcctttgcga
8521 tatgcgtcca tcaactacat gtcgagaagc gaggtttgac agcagagttt gtggaaaaag
8581 gcgacgggac tatatccgct cccgcgaccc gggatgactg gcagtgtgat gtcgtattta
8641 gccttatttt gaacaccaac tttgctcaac atattgacca agatacgtta gttacatcac
8701 taccaaagcg attggctcgg ggttcagcaa aaattgcgat tgatgacttt aaacatatca
8761 actcattctc gacattagaa acagcgatcg aatctctgcc aatagaagct ggtaggtggt
8821 tatcacttta cgcacagtca aacaataatc taagtgatct attagcagcc atgacagagg
8881 accatcagct catggcaagc tgcgtcggtt accacttgtt agaagaaccc aaagataaac
8941 caaactccct cagaggttac aaacacgcta tcgccgagtg catcattgga ctcattaact
9001 caatcacctt tagctcagag actgatccca acacaatctt ttggtcgcta aagaactatc
9061 aaaactacct agtggtacag ccaaggagta tcaacgatga aactaccgac aaatctagcc
9121 tatgagcgct ctatcgaccc atcagatgtc tgtttttttg tcgtctggcc cgatgataga
9181 aaaacacctt taacctacaa ttctcgtact ctgctcgggc aaatggaagc ggcatcatta
9241 gcctatgatg tctcaggtca accaataaaa agtgccaccg ctgaggcgtt agctcaaggg
9301 aaccctcatc aagttgattt ctgccacgtt ccatacggtg cgagtcatat tgaatgcagt
9361 ttctccgttt cgttttcttc tgaactacgt caaccatata agtgtaactc aagcaaagtt
9421 aaacaaacgc tagtgcaatt agtcgagctc tacgaaacga aaatcggctg gactgagcta
9481 gcaacccgat atttgatgaa catttgcaac ggtaaatggc tgtggaaaaa tacccgtaaa
9541 gcctattgct ggaacattgt acttacacct tggccttgga acggggaaaa ggttggattt
9601 gaagatattc gtactaacta cacctcacgg caagacttta aaaataataa aaattggtct
9661 gctatagttg aaatgatcaa aaccgcattt tctagtactg atgggctggc gatatttgaa
9721 gtcagggcca ccttgcactt gccaacgaat gctatggtgc ggccaagcca agttttcaca
9781 gaaaaagaaa gtggcagtaa aagtaaatct aaaactcaaa acagtcgagt ttttcagagt
9841 acaactattg atggtgaacg atcgccaata ctaggggcct ttaaaacggg agcagctatt
9901 gcaaccattg acgactggta tcctgaagcc actgagccac taagggtcgg acggtttggg
9961 gttcatcgcg aagatgtcac ttgctaccgt catccgtcta ccggaaaaga ttttttctcg
10021 atattacaac aagcagagca ctatattgaa gtgttgagcg ccaacaaaac tcccgctcaa
10081 gaaactatca acgacatgca ctttttaatg gctaacctga ttaagggtgg gatgttccag
10141 cataaaggag actgactgtg aaatggtatt ataagacaat cacctttctg ccagagttgt
10201 gcaacaacga gtcactggct gcaaagtgtc tccgcgttct gcatggattt aactatcagt
10261 atgagacacg aaatatcggc gtttcatttc cgctttggtg tgatgcaacg gttggaaaaa
10321 agatttcatt tgtcagcaag aacaagatag aactcgactt actacttaaa caacactatt
10381 tcgtccaaat ggaacaactt caatactttc atatatccaa cactgttctc gtcccagaag
10441 attgtacata cgtttccttt agacgctgtc aatctataga taagctcaca gcagcagggc
10501 tggcaaggaa aatcagacgc ctggagaaac gtgctctatc tagaggcgag caatttgacc
10561 catcatcttt tgctcaaaaa gagcatactg caatagcgca ctaccactca cttggggagt
10621 ccagcaaaca gacgaaccgc aactttcgac tcaatatcag gatgctctcg gagcaacccc
10681 gtgaggggaa ctcgattttt agtagctatg gcttatcaaa ttcagaaaac tcgtttcagc
10741 ctgtaccctt aatctgacct aggctgctgc caccggtgaa ctgccgagta ggtagctgat
10801 aacccctttc gccagctggc gtaatagcga agagggtgaa ctgccgagta ggtagctgat
10861 aacccaaacc agatgtcaac acagctactg ttgatgcaac cataaagtga tatttaataa
10921 ttatttataa tcagcaactt aaccacaaaa caaccatata ttgatatctc acaaaacaac
10981 cataagttga tatttttcag ccaggggtcc ccaataatta cgatttaaat ttgacataag
11041 cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
11101 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
11161 gtttttttgt acagcctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
11221 gatgtttgat gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag
11281 ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacgtgtagg
11341 ctcggccctg accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc
11401 ggtgacgtag ccacctactc ccaacatcag ccggattccg attacctcgg gaacttgctc
11461 cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc
11521 gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg aaatctatat ctatgatctc
11581 gcagtctccg gagagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag
11641 catgaggcca acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat
11701 cccgcagtgg ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc
11761 gacccaagta ccgccaccta acaattcgtt caagccgaga tcggcttccc ggccgcggag
11821 ttgttcggta aattggacaa cggtccgcgc gttgtccttt tccgctgcat aaccctgctt
11881 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
11941 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgaaagtgaa
12001 acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt atttaataat gtgtgcggca
12061 attcacattt aatttatgaa tgttttctta acatcgcggc aactcaagaa acggcaggtt
12121 cggatcttag ctactagaga aagaggagaa atactagatg cgtaaaggcg aggagctgtt
12181 cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc ataagttttc
12241 cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga agttcatctg
12301 tactactggt aaactgccgg ttccttggcc gactctggta acgacgctga cttatggtgt
12361 tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca agtccgccat
12421 gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca cgtacaaaac
12481 gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc tgaaaggcat
12541 tgactttaaa gaggacggca atatcctggg ccataagctg gaatacaatt ttaacagcca
12601 caatgtttac atc
//
