| 质粒名称: | pLH-sgRNA1-MS2-PP7 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75392 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8318 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1-2XMS2-PP7 |
| 物种来源: | Synthetic |
| 插入大小: | 127 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I (未破坏) |
| 3' 克隆位点: | EcoR I (未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1-MS2-PP7 was a gift from Thoru Pederson (Addgene plasmid # 75392 ; http://n2t.net/addgene:75392 ; RRID:Addgene_75392) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
pLH-sgRNA1-MS2-PP7载体质粒基本信息
pLH-sgRNA1-MS2-PP7质粒图谱载体图谱和pLH-sgRNA1-MS2-PP7载体序列质粒序列多克隆位点信息
pLH-sgRNA1-MS2-PP7质粒载体简介
pLH-sgRNA1-MS2-PP7质粒序列载体序列
LOCUS pLH-sgRNA1-MS2-PP7 8318 bp ds-DNA circular SYN 07-MAR-2019
DEFINITION sgRNA1-MS2-PP7.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1-MS2-PP7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8318)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8318)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 7, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8318
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 83..200
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 249..759
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 678..696
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 849..1874
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(1898..1914)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1899..1915)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 1997..2230
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2302..2423
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2339..2358)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2393..2412
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 2463..2598
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2525..2544
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2618..2637)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2619..2637)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2647..2664)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2647..2663)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2656..2678)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2805..3260
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2892..2911)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3102..3123
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3286..3390
/gene="bla"
/label=AmpR promoter
CDS 3391..4251
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(3609..3628)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4422..5010
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4911..4930
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5164..5181
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5298..5319
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5334..5364
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5372..5388
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5377..5399
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5396..5412
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5396..5412
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5431..5451
/label=T3
/note="T3 promoter, forward primer"
promoter 5433..5451
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 5479..5705
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 5706..5886
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5933..6058
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6551..6784
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 7311..7551
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 7311..7331
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 7482..7501
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 7900..8205
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 8084..8103
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_RNA 8243..8261
/label=MS2 stem loop
/note="stem loop that binds the bacteriophage MS2 coat
protein"
ORIGIN
1 tatggcgtcg ctccaagtgg caccgagtcg gtgctttttt tgaattctcg acctcgagac
61 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
121 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
181 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
241 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
301 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
361 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
421 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
481 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
541 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
601 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
661 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
721 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc ggatcaagct
781 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg cagatctgat cccggggggc
841 aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
901 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
961 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
1021 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
1081 ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
1141 tcacgttgca agatctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
1201 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
1261 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
1321 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
1381 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
1441 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
1501 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
1561 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
1621 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
1681 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
1741 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
1801 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
1861 gggcaaagga atagagtaga tgccgaccgg gatctatcga taccgtcgac ctcgaggggg
1921 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
1981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagaaaaga tctgcttttt
2041 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
2101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
2161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
2221 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
2281 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
2341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
2401 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
2461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
2521 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
2581 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
2641 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
2701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
2761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
2821 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
2881 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
2941 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
3001 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
3061 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3121 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
3181 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
3241 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
3301 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
3361 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
3421 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
3481 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
3541 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
3601 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
3661 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
3721 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
3781 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
3841 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
3901 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
3961 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
4021 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
4081 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
4141 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
4201 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
4261 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
4321 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
4381 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
4441 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
4501 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
4561 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
4621 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
4681 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
4741 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
4801 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
4861 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
4921 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
4981 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
5041 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
5101 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
5161 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
5221 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
5281 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
5341 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
5401 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
5461 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
5521 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
5581 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
5641 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
5701 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
5761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
5821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
5881 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
5941 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
6001 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
6061 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
6121 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
6181 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
6241 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
6301 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
6361 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
6421 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
6481 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
6541 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
6601 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
6661 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
6721 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
6781 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
6841 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
6901 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
6961 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
7021 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
7081 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
7141 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
7201 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
7261 ctcgacggta tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat
7321 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa
7381 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
7441 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
7501 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
7561 gtgtcttcga ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
7621 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg
7681 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
7741 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
7801 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
7861 tgaacggctc ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc
7921 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
7981 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
8041 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
8101 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
8161 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaaccct gcaggacgaa
8221 gacccgtttg agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt
8281 caaataaggc tagtccgtta tcaacttgga gcagacga
//
